Interactions of acetylated histones with DNA as revealed by UV laser induced histone-DNA crosslinking

V. Yu Stefanovsky, S. I. Dimitrov, D. Angelov, I. G. Pashev

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Abstract

The interaction of acetylated histones with DNA in chromatin has been studied by UV laser-induced crosslinking histones to DNA. After irradiation of the nuclei, the covalently linked protein-DNA complexes were isolated and the presence of histones in them demonstrated immunochemically. When chromatin from irradiated nuclei was treated with clostripain, which selectively cleaved the N-terminal tails of core histones, no one of them was found covalently linked to DNA, thus showing that crosslinking proceeded solely via the N-terminal regions. However, the crosslinking ability of the laser was preserved both upon physiological acetylation of histones, known to be restricted to the N-terminal tails, and with chemically acetylated chromatin. This finding is direct evidence that the postsynthetic histone acetylation does not release the N-terminal tails from interaction with DNA.

Original languageEnglish
Pages (from-to)304-310
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume164
Issue number1
DOIs
Publication statusPublished - 16 Oct 1989
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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