Interaction of Pneumocystis carinii with dendritic cells and resulting host responses to P. carinii

Hiroyasu Kobayashi, Stefan Worgall, Timothy P. O'Connor, Ronald Crystal

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To assess the interaction of Pneumocystis carinii with dendritic cells (DCs), and the consequences of the response of the host immune system to P. carinii antigens when DC are pulsed with P. carinii, murine DC were pulsed with P. carinii, and the resultant P. carinii host responses assessed in vitro and in vivo. P. carinii interacted with murine bone marrow-derived DC in vitro in part via mannose receptors. DC pulsed with P. carinii did not demonstrate increased expression of the cell surface markers MHC II, CD40, CD54, CD80 (B7.1), and CD86 (B7.2). The release of interleukin (IL)-4 was increased, but there was no increase in the release of interleukin (IL)-12p40, IL-10, tumor necrosis factor-α, IL-6, and nitrite compared with naive DC. In vivo administration of DC pulsed with P. carinii induced a P. carinii-specific response, generating CD4 cells that proliferated and released IL-4, but not interferon-γ, in response to P. carinii-pulsed DC in vitro. In vivo administration of DC pulsed with P. carinii also induced P. carinii-specific immunoglobulin (Ig)G1, IgG2a, and IgG2b, but not IgG3, antibodies in serum, and lung lavage fluid. Finally, CD4 depleted mice immunized with DC pulsed with P. carinii demonstrated suppression of lung growth of P. carinii after intratracheal challenge with P. carinii at 3 and 16 weeks after immunization. These observations provide insight into DC-P. carinii interactions, and support the concept that a vaccine that includes DC pulsed with P. carinii can mount a humoral and T helper 2-type cellular response to P. carinii sufficient to suppress the growth of P. carinii in the lung.

Original languageEnglish
Pages (from-to)54-63
Number of pages10
JournalJournal of Immunotherapy
Volume30
Issue number1
DOIs
Publication statusPublished - 1 Jan 2007
Externally publishedYes

Fingerprint

Pneumocystis carinii
Dendritic Cells
Interleukin-4
Lung

Keywords

  • Dendritic cells
  • Host responses
  • Immunity
  • P. carinni

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology
  • Cancer Research

Cite this

Interaction of Pneumocystis carinii with dendritic cells and resulting host responses to P. carinii. / Kobayashi, Hiroyasu; Worgall, Stefan; O'Connor, Timothy P.; Crystal, Ronald.

In: Journal of Immunotherapy, Vol. 30, No. 1, 01.01.2007, p. 54-63.

Research output: Contribution to journalArticle

Kobayashi, Hiroyasu ; Worgall, Stefan ; O'Connor, Timothy P. ; Crystal, Ronald. / Interaction of Pneumocystis carinii with dendritic cells and resulting host responses to P. carinii. In: Journal of Immunotherapy. 2007 ; Vol. 30, No. 1. pp. 54-63.
@article{0a0c620632494e03b979a426b0ed67b7,
title = "Interaction of Pneumocystis carinii with dendritic cells and resulting host responses to P. carinii",
abstract = "To assess the interaction of Pneumocystis carinii with dendritic cells (DCs), and the consequences of the response of the host immune system to P. carinii antigens when DC are pulsed with P. carinii, murine DC were pulsed with P. carinii, and the resultant P. carinii host responses assessed in vitro and in vivo. P. carinii interacted with murine bone marrow-derived DC in vitro in part via mannose receptors. DC pulsed with P. carinii did not demonstrate increased expression of the cell surface markers MHC II, CD40, CD54, CD80 (B7.1), and CD86 (B7.2). The release of interleukin (IL)-4 was increased, but there was no increase in the release of interleukin (IL)-12p40, IL-10, tumor necrosis factor-α, IL-6, and nitrite compared with naive DC. In vivo administration of DC pulsed with P. carinii induced a P. carinii-specific response, generating CD4 cells that proliferated and released IL-4, but not interferon-γ, in response to P. carinii-pulsed DC in vitro. In vivo administration of DC pulsed with P. carinii also induced P. carinii-specific immunoglobulin (Ig)G1, IgG2a, and IgG2b, but not IgG3, antibodies in serum, and lung lavage fluid. Finally, CD4 depleted mice immunized with DC pulsed with P. carinii demonstrated suppression of lung growth of P. carinii after intratracheal challenge with P. carinii at 3 and 16 weeks after immunization. These observations provide insight into DC-P. carinii interactions, and support the concept that a vaccine that includes DC pulsed with P. carinii can mount a humoral and T helper 2-type cellular response to P. carinii sufficient to suppress the growth of P. carinii in the lung.",
keywords = "Dendritic cells, Host responses, Immunity, P. carinni",
author = "Hiroyasu Kobayashi and Stefan Worgall and O'Connor, {Timothy P.} and Ronald Crystal",
year = "2007",
month = "1",
day = "1",
doi = "10.1097/01.cji.0000211314.13492.64",
language = "English",
volume = "30",
pages = "54--63",
journal = "Journal of Immunotherapy",
issn = "1524-9557",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Interaction of Pneumocystis carinii with dendritic cells and resulting host responses to P. carinii

AU - Kobayashi, Hiroyasu

AU - Worgall, Stefan

AU - O'Connor, Timothy P.

AU - Crystal, Ronald

PY - 2007/1/1

Y1 - 2007/1/1

N2 - To assess the interaction of Pneumocystis carinii with dendritic cells (DCs), and the consequences of the response of the host immune system to P. carinii antigens when DC are pulsed with P. carinii, murine DC were pulsed with P. carinii, and the resultant P. carinii host responses assessed in vitro and in vivo. P. carinii interacted with murine bone marrow-derived DC in vitro in part via mannose receptors. DC pulsed with P. carinii did not demonstrate increased expression of the cell surface markers MHC II, CD40, CD54, CD80 (B7.1), and CD86 (B7.2). The release of interleukin (IL)-4 was increased, but there was no increase in the release of interleukin (IL)-12p40, IL-10, tumor necrosis factor-α, IL-6, and nitrite compared with naive DC. In vivo administration of DC pulsed with P. carinii induced a P. carinii-specific response, generating CD4 cells that proliferated and released IL-4, but not interferon-γ, in response to P. carinii-pulsed DC in vitro. In vivo administration of DC pulsed with P. carinii also induced P. carinii-specific immunoglobulin (Ig)G1, IgG2a, and IgG2b, but not IgG3, antibodies in serum, and lung lavage fluid. Finally, CD4 depleted mice immunized with DC pulsed with P. carinii demonstrated suppression of lung growth of P. carinii after intratracheal challenge with P. carinii at 3 and 16 weeks after immunization. These observations provide insight into DC-P. carinii interactions, and support the concept that a vaccine that includes DC pulsed with P. carinii can mount a humoral and T helper 2-type cellular response to P. carinii sufficient to suppress the growth of P. carinii in the lung.

AB - To assess the interaction of Pneumocystis carinii with dendritic cells (DCs), and the consequences of the response of the host immune system to P. carinii antigens when DC are pulsed with P. carinii, murine DC were pulsed with P. carinii, and the resultant P. carinii host responses assessed in vitro and in vivo. P. carinii interacted with murine bone marrow-derived DC in vitro in part via mannose receptors. DC pulsed with P. carinii did not demonstrate increased expression of the cell surface markers MHC II, CD40, CD54, CD80 (B7.1), and CD86 (B7.2). The release of interleukin (IL)-4 was increased, but there was no increase in the release of interleukin (IL)-12p40, IL-10, tumor necrosis factor-α, IL-6, and nitrite compared with naive DC. In vivo administration of DC pulsed with P. carinii induced a P. carinii-specific response, generating CD4 cells that proliferated and released IL-4, but not interferon-γ, in response to P. carinii-pulsed DC in vitro. In vivo administration of DC pulsed with P. carinii also induced P. carinii-specific immunoglobulin (Ig)G1, IgG2a, and IgG2b, but not IgG3, antibodies in serum, and lung lavage fluid. Finally, CD4 depleted mice immunized with DC pulsed with P. carinii demonstrated suppression of lung growth of P. carinii after intratracheal challenge with P. carinii at 3 and 16 weeks after immunization. These observations provide insight into DC-P. carinii interactions, and support the concept that a vaccine that includes DC pulsed with P. carinii can mount a humoral and T helper 2-type cellular response to P. carinii sufficient to suppress the growth of P. carinii in the lung.

KW - Dendritic cells

KW - Host responses

KW - Immunity

KW - P. carinni

UR - http://www.scopus.com/inward/record.url?scp=33845978430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845978430&partnerID=8YFLogxK

U2 - 10.1097/01.cji.0000211314.13492.64

DO - 10.1097/01.cji.0000211314.13492.64

M3 - Article

C2 - 17198083

AN - SCOPUS:33845978430

VL - 30

SP - 54

EP - 63

JO - Journal of Immunotherapy

JF - Journal of Immunotherapy

SN - 1524-9557

IS - 1

ER -