Integrative transcript and metabolite analysis of nutritionally enhanced DE-ETIOLATED1 downregulated tomato fruit

Eugenia M.A. Enfissi, Fredy Barneche, Ikhlak Ahmed, Christiane Lichtlé, Christopher Gerrish, Ryan P. McQuinn, James J. Giovannoni, Enrique Lopez-Juez, Chris Bowler, Peter M. Bramley, Paul D. Fraser

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Fruit-specific downregulation of the DE-ETIOLATED1 (DET1) gene product results in tomato fruits (Solanum lycopersicum) containing enhanced nutritional antioxidants, with no detrimental effects on yield. In an attempt to further our understanding of how modulation of this gene leads to improved quality traits, detailed targeted and multilevel omic characterization has been performed. Metabolite profiling revealed quantitative increases in carotenoid, tocopherol, phenylpropanoids, flavonoids, and anthocyanidins. Qualitative differences could also be identified within the phenolics, including unique formation in fruit pericarp tissues. These changes resulted in increased total antioxidant content both in the polar and nonpolar fractions. Increased transcription of key biosynthetic genes is a likely mechanism producing elevated phenolic-based metabolites. By contrast, high levels of isoprenoids do not appear to result from transcriptional regulation but are more likely related to plastid-based parameters, such as increased plastid volume per cell. Parallel metabolomic and transcriptomic analyses reveal the widespread effects of DET1 downregulation on diverse sectors of metabolism and sites of synthesis. Correlation analysis of transcripts and metabolites independently indicated strong coresponses within and between related pathways/processes. Interestingly, despite the fact that secondary metabolites were the most severely affected in ripe tomato fruit, our integrative analyses suggest that the coordinated activation of core metabolic processes in cell types amenable to plastid biogenesis is the main effect of DET1 loss of function.

Original languageEnglish
Pages (from-to)1190-1215
Number of pages26
JournalPlant Cell
Volume22
Issue number4
DOIs
Publication statusPublished - 1 Apr 2010
Externally publishedYes

Fingerprint

Lycopersicon esculentum
Plastids
Fruit
Down-Regulation
tomatoes
metabolites
plastids
fruits
Antioxidants
Genes
antioxidants
anthocyanidins
Metabolomics
Tocopherols
genes
Anthocyanins
isoprenoids
Terpenes
Solanum lycopersicum
metabolomics

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Enfissi, E. M. A., Barneche, F., Ahmed, I., Lichtlé, C., Gerrish, C., McQuinn, R. P., ... Fraser, P. D. (2010). Integrative transcript and metabolite analysis of nutritionally enhanced DE-ETIOLATED1 downregulated tomato fruit. Plant Cell, 22(4), 1190-1215. https://doi.org/10.1105/tpc.110.073866

Integrative transcript and metabolite analysis of nutritionally enhanced DE-ETIOLATED1 downregulated tomato fruit. / Enfissi, Eugenia M.A.; Barneche, Fredy; Ahmed, Ikhlak; Lichtlé, Christiane; Gerrish, Christopher; McQuinn, Ryan P.; Giovannoni, James J.; Lopez-Juez, Enrique; Bowler, Chris; Bramley, Peter M.; Fraser, Paul D.

In: Plant Cell, Vol. 22, No. 4, 01.04.2010, p. 1190-1215.

Research output: Contribution to journalArticle

Enfissi, EMA, Barneche, F, Ahmed, I, Lichtlé, C, Gerrish, C, McQuinn, RP, Giovannoni, JJ, Lopez-Juez, E, Bowler, C, Bramley, PM & Fraser, PD 2010, 'Integrative transcript and metabolite analysis of nutritionally enhanced DE-ETIOLATED1 downregulated tomato fruit', Plant Cell, vol. 22, no. 4, pp. 1190-1215. https://doi.org/10.1105/tpc.110.073866
Enfissi, Eugenia M.A. ; Barneche, Fredy ; Ahmed, Ikhlak ; Lichtlé, Christiane ; Gerrish, Christopher ; McQuinn, Ryan P. ; Giovannoni, James J. ; Lopez-Juez, Enrique ; Bowler, Chris ; Bramley, Peter M. ; Fraser, Paul D. / Integrative transcript and metabolite analysis of nutritionally enhanced DE-ETIOLATED1 downregulated tomato fruit. In: Plant Cell. 2010 ; Vol. 22, No. 4. pp. 1190-1215.
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