Initiation of Globin Synthesis in β-Thalassemia

Ronald Crystal, Norton A. Elson, Arthur Nienhuis, Abigail C. Thornton, W. French Anderson

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The mechanism of initiation of human globin chains was examined to determine if the initiation of protein synthesis is abnormal in β-thalassemia. Messenger RNA (mRNA) was isolated from the reticulocytes of normal adults, newborn infants and patients with β-thalassemia to study synthesis of alpha, beta and gamma chains. These isolated mRNA preparations were assayed in a cell-free system derived from rabbit reticulocytes. The initial dipeptides formed during the synthesis of the alpha and beta chains were determined. In normal subjects and patients with β-thalassemia the dipeptide for both the alpha and beta chains was found to be methionine-valine. The initial dipeptide for the gamma chain, determined in newborn infants and in patients with β-thalassemia, was methionine-glycine in both. All mRNA's studied shared a common requirement for methionyl-tRNAF (the specific initiator tRNA) and for the protein initiation factors. By these criteria, the mechanism of globin-chain initiation in β-thalassemia is normal.

Original languageEnglish
Pages (from-to)1091-1096
Number of pages6
JournalNew England Journal of Medicine
Volume288
Issue number21
DOIs
Publication statusPublished - 24 May 1973
Externally publishedYes

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Thalassemia
Globins
Dipeptides
Reticulocytes
Methionine
Messenger RNA
Newborn Infant
RNA, Transfer, Met
Peptide Initiation Factors
Cell-Free System
Valine
Glycine
Proteins
Rabbits

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Crystal, R., Elson, N. A., Nienhuis, A., Thornton, A. C., & Anderson, W. F. (1973). Initiation of Globin Synthesis in β-Thalassemia. New England Journal of Medicine, 288(21), 1091-1096. https://doi.org/10.1056/NEJM197305242882103

Initiation of Globin Synthesis in β-Thalassemia. / Crystal, Ronald; Elson, Norton A.; Nienhuis, Arthur; Thornton, Abigail C.; Anderson, W. French.

In: New England Journal of Medicine, Vol. 288, No. 21, 24.05.1973, p. 1091-1096.

Research output: Contribution to journalArticle

Crystal, R, Elson, NA, Nienhuis, A, Thornton, AC & Anderson, WF 1973, 'Initiation of Globin Synthesis in β-Thalassemia', New England Journal of Medicine, vol. 288, no. 21, pp. 1091-1096. https://doi.org/10.1056/NEJM197305242882103
Crystal, Ronald ; Elson, Norton A. ; Nienhuis, Arthur ; Thornton, Abigail C. ; Anderson, W. French. / Initiation of Globin Synthesis in β-Thalassemia. In: New England Journal of Medicine. 1973 ; Vol. 288, No. 21. pp. 1091-1096.
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