Inhibition of HIV-1 replication in alveolar macrophages by adenovirus gene transfer vectors

Joshua Rice, Ruth Connor, Stefan Worgall, John P. Moore, Philip L. Leopold, Robert J. Kaner, Ronald Crystal

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To assess the hypothesis that infection of alveolar macrophages (AM) with adenovirus (Ad) gene transfer vectors might prevent subsequent human immunodeficiency virus (HIV)-I replication in AM, AM isolated from normal volunteers were infected with increasing doses of first generation (E1-) Ad vectors, followed 72 h later by infection with HIV-1JRFL, an R5/M-tropic strain that preferentially uses the CCR5 coreceptor. As a measure of HIV-1 replication, p24 Ag was quantified by enzyme-linked imunosorbent assay in supernatants on Days 4 to 14 after HIV-1 infection. Pretreatment of the AM with an Ad vector resulted in a dose- and time-dependent suppression of subsequent HIV-1 replication. The Ad vector inhibition of HIV-1 replication was independent of the transgene in the Ad vector expression cassette and E4 genes in the Ad backbone. Moreover, it did not appear to be secondary to a soluble factor released by the AM, nor was it overridden by the concomitant transfer of the CCR5 or CXCR4 receptors to the AM before HIV-1 infection. These observations have implications regarding pulmonary host responses associated with HIV-1 infection, as well as possibly uncovering new therapeutic strategies against HIV-1 infection.

Original languageEnglish
Pages (from-to)214-219
Number of pages6
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume27
Issue number2
DOIs
Publication statusPublished - 1 Jan 2002
Externally publishedYes

Fingerprint

Gene transfer
Alveolar Macrophages
Virus Replication
Viruses
Adenoviridae
HIV-1
Virus Diseases
Genes
CCR5 Receptors
HIV
CXCR4 Receptors
Infection
Tropics
Transgenes
Healthy Volunteers
Assays
Lung
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Inhibition of HIV-1 replication in alveolar macrophages by adenovirus gene transfer vectors. / Rice, Joshua; Connor, Ruth; Worgall, Stefan; Moore, John P.; Leopold, Philip L.; Kaner, Robert J.; Crystal, Ronald.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 27, No. 2, 01.01.2002, p. 214-219.

Research output: Contribution to journalArticle

Rice, Joshua ; Connor, Ruth ; Worgall, Stefan ; Moore, John P. ; Leopold, Philip L. ; Kaner, Robert J. ; Crystal, Ronald. / Inhibition of HIV-1 replication in alveolar macrophages by adenovirus gene transfer vectors. In: American Journal of Respiratory Cell and Molecular Biology. 2002 ; Vol. 27, No. 2. pp. 214-219.
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