Influence of the β-sheet content on the mechanical properties of aggregates during amyloid fibrillization

Francesco Simone Ruggeri, Jozef Adamcik, Jae Sun Jeong, Hilal A. Lashuel, Raffaele Mezzenga, Giovanni Dietler

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Amyloid fibrils associated with neurodegenerative diseases, such as Parkinson's and Alzheimer's, consist of insoluble aggregates of α-synuclein and Aβ-42 proteins with a high β-sheet content. The aggregation of both proteins occurs by misfolding of the monomers and proceeds through the formation of intermediate oligomeric and protofibrillar species to give the final fibrillar cross-β-sheet structure. The morphological and mechanical properties of oligomers, protofibrils, and fibrils formed during the fibrillization process were investigated by thioflavin T fluorescence and circular dichroism in combination with AFM peak force quantitative nanomechanical technique. The results reveal an increase in the Young's modulus during the transformation from oligomers to mature fibrils, thus inferring that the difference in their mechanical properties is due to an internal structural change from a random coil to a structure with increased β-sheet content.

Original languageEnglish
Pages (from-to)2462-2466
Number of pages5
JournalAngewandte Chemie - International Edition
Volume54
Issue number8
DOIs
Publication statusPublished - 16 Feb 2015
Externally publishedYes

Fingerprint

Oligomers
Amyloid
Synucleins
Neurodegenerative diseases
Proteins
Mechanical properties
Elastic Modulus
Dichroism
Circular Dichroism
Neurodegenerative Diseases
Agglomeration
Monomers
Elastic moduli
Fluorescence
thioflavin T

Keywords

  • Amyloids
  • Mechanical properties
  • Nanomaterials
  • Neurodegenerative disorders
  • Scanning probe microscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Influence of the β-sheet content on the mechanical properties of aggregates during amyloid fibrillization. / Ruggeri, Francesco Simone; Adamcik, Jozef; Jeong, Jae Sun; Lashuel, Hilal A.; Mezzenga, Raffaele; Dietler, Giovanni.

In: Angewandte Chemie - International Edition, Vol. 54, No. 8, 16.02.2015, p. 2462-2466.

Research output: Contribution to journalArticle

Ruggeri, Francesco Simone ; Adamcik, Jozef ; Jeong, Jae Sun ; Lashuel, Hilal A. ; Mezzenga, Raffaele ; Dietler, Giovanni. / Influence of the β-sheet content on the mechanical properties of aggregates during amyloid fibrillization. In: Angewandte Chemie - International Edition. 2015 ; Vol. 54, No. 8. pp. 2462-2466.
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