Induction of the hair growth phase in postnatal mice by localized transient expression of Sonic hedgehog

Noboru Sato, Philip L. Leopold, Ronald Crystal

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

Hair follicles form in prenatal skin and mature in the postnatal period, establishing a growth cycle in 3 phases: telogen (resting), anagen (growth), and catagen (regression). Based on the knowledge that Sonic hedgehog (Shh) expression is necessary for the embryonic development of hair follicles, and that anagen in the postnatal cycling follicle has morphologic similarities to the epithelial invagination process in embryonic skin, we hypothesized that localized, but transient, enhanced expression of the Shh gene in postnatal skin would accelerate initiation of anagen in the hair follicle cycle, with concomitant accelerated hair growth. To assess this concept, an E1- adenovirus vector, AdShh, was used to transfer the murine Shh cDNA to skin of postnatal day 19 C57BL/6 mice. The treated skin showed increased mRNA expression of Shh, Patched (the Shh receptor), and Gli1 (a transcription factor in the Shh pathway). In mice receiving AdShh, but not in controls, acceleration into anagen was evident, since hair follicle size and melanogenesis increased and the hair-specific keratin ghHb-1 and the melanin synthesis-related tyrosinase mRNAs accumulated. Finally, C57BL/6 mice showed marked acceleration of the onset of new hair growth in the region of AdShh administration to skin 2 weeks after treatment, but not in control vector- treated or untreated areas. After 6 months, AdShh-treated skin showed normal hair and normal skin morphology. Together, these observations are consistent with the concept that upregulation of Shh activity in postnatal skin functions as a biologic switch that induces resting hair follicles to enter anagen with consequent hair growth.

Original languageEnglish
Pages (from-to)855-864
Number of pages10
JournalJournal of Clinical Investigation
Volume104
Issue number7
DOIs
Publication statusPublished - 1 Jan 1999
Externally publishedYes

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Hedgehogs
Hair
Hair Follicle
Skin
Growth
Inbred C57BL Mouse
Hair-Specific Keratins
Keratin-1
Messenger RNA
Monophenol Monooxygenase
Melanins
Adenoviridae
Embryonic Development
Transcription Factors
Up-Regulation
Complementary DNA

ASJC Scopus subject areas

  • Medicine(all)

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Induction of the hair growth phase in postnatal mice by localized transient expression of Sonic hedgehog. / Sato, Noboru; Leopold, Philip L.; Crystal, Ronald.

In: Journal of Clinical Investigation, Vol. 104, No. 7, 01.01.1999, p. 855-864.

Research output: Contribution to journalArticle

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