Induction of pro-inflammatory programs in enteroendocrine cells by the Toll-like receptor agonists flagellin and bacterial LPS

Silvia Selleri, Marco Palazzo, Sara Deola, Ena Wang, Andrea Balsari, Francesco M. Marincola, Cristiano Rumio

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Enteroendocrine cells are hormone-secreting cells spread along the intestinal epithelium. Their principal function is to promote the digestion of food. However, little is known about other functions that these cells may play, since they are difficult to study as a whole endocrine organ due to their diffuse localization. It is known that the intestinal epithelial barrier is actively involved in the host defense against pathogen invasion. Here we applied gene expression profiling to characterize the response of the human LCC-18 enteroendocrine cell line to physiological and pathological stimuli mimicked by fatty acids (FAs), flagellin and LPS exposure. We observed that these cells participate in an innate immune reaction to pathogens through the expression of pro-inflammatory factors (i.e. CXCL1 and 3 and IL-32) that we could validate by molecular and proteomic approach. Interestingly, IL-32 has been recently found over-expressed in the inflamed mucosa of patients affected by inflammatory bowel disease. This is very important because modifications of enteroendocrine cells during intestinal inflammation have been so far considered as secondary effects of the inflammatory status rather than due to direct pathogen/enteroendocrine cell interaction. As expected, FAs exposure up-regulates pro-differentiative genes and the production of cholecystokinin but it does not enhance the expression of pro-inflammatory genes. The present observations enlighten a new aspect of the cross talk between immune and endocrine system and suggest enteroendocrine cells as important contributors of inflammatory processes occurring in the gut in response to pathogen exposure and direct enhancers of the inflammatory status associated with human inflammatory bowel disease.

Original languageEnglish
Pages (from-to)961-970
Number of pages10
JournalInternational Immunology
Volume20
Issue number8
DOIs
Publication statusPublished - Aug 2008
Externally publishedYes

Fingerprint

Enteroendocrine Cells
Flagellin
Toll-Like Receptors
Inflammatory Bowel Diseases
Fatty Acids
Endocrine System
Interleukin-3
Cholecystokinin
Gene Expression Profiling
Intestinal Mucosa
Cell Communication
Proteomics
Genes
Digestion
Immune System
Mucous Membrane
Up-Regulation
Hormones
Inflammation
Cell Line

Keywords

  • Enteroendocrine cells
  • Inflammation
  • Intestine
  • TLR

ASJC Scopus subject areas

  • Immunology

Cite this

Induction of pro-inflammatory programs in enteroendocrine cells by the Toll-like receptor agonists flagellin and bacterial LPS. / Selleri, Silvia; Palazzo, Marco; Deola, Sara; Wang, Ena; Balsari, Andrea; Marincola, Francesco M.; Rumio, Cristiano.

In: International Immunology, Vol. 20, No. 8, 08.2008, p. 961-970.

Research output: Contribution to journalArticle

Selleri, Silvia ; Palazzo, Marco ; Deola, Sara ; Wang, Ena ; Balsari, Andrea ; Marincola, Francesco M. ; Rumio, Cristiano. / Induction of pro-inflammatory programs in enteroendocrine cells by the Toll-like receptor agonists flagellin and bacterial LPS. In: International Immunology. 2008 ; Vol. 20, No. 8. pp. 961-970.
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