Induction of immune alterations and successful renal transplantation with a simplified method of donor–specific blood transfusion

Jhoong S. Cheigh, Manikkam Suthanthiran, Matthew Kaplan, Monica Evelyn, Robert R. Riggio, Marilena Fotino, Naomi Schechter, Carl F.W. Wolf, William T. Stubenbord, Kurt H. Stenzel, Albert L. Rubin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We developed a new and simplified donor-specific blood transfusion (DSBT) protocol for prospective kidney transplant recipients from one-haplotype-mismatched related donors. Prospective kidney donors gave 450 ml of blood in a quad-pack unit, and the blood was stored in a blood bank. Twenty-five patients were transfused with 100 ml of the respective donors whole blood at 1, 8, and 15 days after its storage. After DSBT, only three (12state) developed donor-specific lymphocytotoxic antibodies. Following DSBT, donor-specific mixed lymphocyte culture (MLC) was significantly suppressed, without any accelerated (secondary-type) response in early MLC. In addition, sera obtained after DSBT also suppressed donor-specific MLC significantly. Sixteen recipients subsequently received a kidney transplant from the donor, and all had functioning grafts at three months, but one lost the graft thereafter (graft survival rate: 94state at 12 months). This study indicates that (1) 100 ml of stored whole-blood DSBT three times at weekly intervals is a practical, less immunizing, and effective approach to enhance graft survival in recipients of a one-haplotype-mismatched graft; and (2) immune consequences of DSBT include induction of donor-specific cellular and humoral adaptive responses that might be conducive to successful graft outcome.

Original languageEnglish
Pages (from-to)501-505
Number of pages5
JournalTransplantation
Volume38
Issue number5
DOIs
Publication statusPublished - 1 Jan 1984
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Transplantation

Cite this

Cheigh, J. S., Suthanthiran, M., Kaplan, M., Evelyn, M., Riggio, R. R., Fotino, M., Schechter, N., Wolf, C. F. W., Stubenbord, W. T., Stenzel, K. H., & Rubin, A. L. (1984). Induction of immune alterations and successful renal transplantation with a simplified method of donor–specific blood transfusion. Transplantation, 38(5), 501-505. https://doi.org/10.1097/00007890-198411000-00012