Induction of chromosomal aberrations by the rhodium(III) complex cis-[Rh(biq)2Cl2]Cl in cultured human lymphocytes

May F. Sadiq, Mukarram H. Zaghal, Hatem E. El-Shanti

Research output: Contribution to journalArticle

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Abstract

The genotoxicity of the rhodium(III) complex cis-[Rh(biq)2Cl2]Cl (complex R) in cultured human lymphocytes was studied using the chromosome aberrations (CAs) assay. Lymphocyte cultures were initiated from two adult healthy non-smoking male volunteers and were exposed to the complex for a duration of 3 or 20 h prior to cell collection. The reduction in mitotic indices (MI) and the induction of CAs represented the toxic and clastogenic effects of the different treatments, respectively. Complex R proved to be an intermediate toxic clastogen with a MI50 of 1.0 μg/ml and a minimum positive dose (MPD) of 0.1 μg/ml. Like bleomycin, complex R exerted its clastogenic effects without the need for metabolic activation and induced CAs of all types in lymphocytes treated in the G2 and late S phases and, therefore, can be considered a radiomimetic. In addition, it induced more total CAs of chromatid-type than of chromosome-type. The reduction in the frequencies of CAs following the 20 h treatment as compared with those induced following the 3 h treatments indicated that human lymphocytes in the presence of complex R can partially tolerate the lesions involved in CA production. Based on the biological effects of complex R and the similarities between its functional group and that of bleomycin, possible mechanisms for complex R genotoxicity are discussed.

Original languageEnglish
Pages (from-to)375-378
Number of pages4
JournalMutagenesis
Volume15
Issue number5
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Rhodium
Lymphocytes
Chromosomes
Aberrations
Chromosome Aberrations
Poisons
Bleomycin
Mitotic Index
Chromatids
Mutagens
complex R
S Phase
Cell culture
Functional groups
Volunteers
Assays
Therapeutics
Chemical activation

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Induction of chromosomal aberrations by the rhodium(III) complex cis-[Rh(biq)2Cl2]Cl in cultured human lymphocytes. / Sadiq, May F.; Zaghal, Mukarram H.; El-Shanti, Hatem E.

In: Mutagenesis, Vol. 15, No. 5, 2000, p. 375-378.

Research output: Contribution to journalArticle

Sadiq, May F. ; Zaghal, Mukarram H. ; El-Shanti, Hatem E. / Induction of chromosomal aberrations by the rhodium(III) complex cis-[Rh(biq)2Cl2]Cl in cultured human lymphocytes. In: Mutagenesis. 2000 ; Vol. 15, No. 5. pp. 375-378.
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