Increased expression of protease-activated receptor 1 (PAR-1) in human leukemias

Camilla de S.B. Veiga, Tatiana Lobo, Cláudia J.B.P. Coelho, Silvia M.F. Carvalho, Raquel C. Maia, Flávia C. Vasconcelos, Eliana Abdelhay, André L. Mencalha, Aline F. Ferreira, Fabíola A. Castro, Robson Q. Monteiro

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Protease-activated receptor 1 (PAR-1) is a G-protein-coupled receptor that is overexpressed in solid tumors, being associated with several pro-tumoral responses including primary growth, invasion, metastasis and angiogenesis. Expression of PAR-1 in human leukemic cell lines is reported but the status of its expression in human leukemic patients is currently unknown. In this study we evaluated the expression pattern of PAR-1 in patients with the four main types of leukemia - chronic lymphocytic leukemia subtype B (B-CLL), acute lymphoblastic leukemia subtype B (B-ALL), acute myeloid leukemia (AML) and chronic myeloid leukemia (CML). Flow cytometry analyses show that lymphocytes from B-CLL patients express this receptor at similar levels to healthy individuals. On the other hand, it was observed a significant increase in PAR-1 expression in B-ALL lymphocytes as compared to B-CLL and healthy donors. Flow cytometric and real-time PCR demonstrated a significant increase in PAR-1 expression in granulocytes from CML patients in blast phase (CML-BP) but not in chronic phase (CML-CP) as compared to healthy donors. Finally, a significant increase in PAR-1 expression has been also observed in blasts from AML (subtypes M4 and M5) patients, as compared to monocytes or granulocytes from healthy donors. We conclude that PAR-1 might play an important biological role in aggressive leukemias and might offer additional strategies for the development of new therapies.

Original languageEnglish
Pages (from-to)230-234
Number of pages5
JournalBlood Cells, Molecules, and Diseases
Volume46
Issue number3
DOIs
Publication statusPublished - 15 Mar 2011
Externally publishedYes

Fingerprint

PAR-1 Receptor
Leukemia
Tissue Donors
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Granulocytes
Leukemia, Myelomonocytic, Acute
Leukemia, Monocytic, Acute
Leukemia, Myeloid, Chronic Phase
Blast Crisis
B-Cell Chronic Lymphocytic Leukemia
G-Protein-Coupled Receptors
Acute Myeloid Leukemia
Real-Time Polymerase Chain Reaction
Monocytes
Flow Cytometry
B-Lymphocytes
Lymphocytes
Neoplasm Metastasis
Cell Line

Keywords

  • Acute myeloid leukemia
  • Chronic myeloid leukemia
  • Protease-activated receptor 1 (PAR-1)
  • Thrombin

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Hematology
  • Cell Biology

Cite this

Veiga, C. D. S. B., Lobo, T., Coelho, C. J. B. P., Carvalho, S. M. F., Maia, R. C., Vasconcelos, F. C., ... Monteiro, R. Q. (2011). Increased expression of protease-activated receptor 1 (PAR-1) in human leukemias. Blood Cells, Molecules, and Diseases, 46(3), 230-234. https://doi.org/10.1016/j.bcmd.2010.12.005

Increased expression of protease-activated receptor 1 (PAR-1) in human leukemias. / Veiga, Camilla de S.B.; Lobo, Tatiana; Coelho, Cláudia J.B.P.; Carvalho, Silvia M.F.; Maia, Raquel C.; Vasconcelos, Flávia C.; Abdelhay, Eliana; Mencalha, André L.; Ferreira, Aline F.; Castro, Fabíola A.; Monteiro, Robson Q.

In: Blood Cells, Molecules, and Diseases, Vol. 46, No. 3, 15.03.2011, p. 230-234.

Research output: Contribution to journalArticle

Veiga, CDSB, Lobo, T, Coelho, CJBP, Carvalho, SMF, Maia, RC, Vasconcelos, FC, Abdelhay, E, Mencalha, AL, Ferreira, AF, Castro, FA & Monteiro, RQ 2011, 'Increased expression of protease-activated receptor 1 (PAR-1) in human leukemias', Blood Cells, Molecules, and Diseases, vol. 46, no. 3, pp. 230-234. https://doi.org/10.1016/j.bcmd.2010.12.005
Veiga, Camilla de S.B. ; Lobo, Tatiana ; Coelho, Cláudia J.B.P. ; Carvalho, Silvia M.F. ; Maia, Raquel C. ; Vasconcelos, Flávia C. ; Abdelhay, Eliana ; Mencalha, André L. ; Ferreira, Aline F. ; Castro, Fabíola A. ; Monteiro, Robson Q. / Increased expression of protease-activated receptor 1 (PAR-1) in human leukemias. In: Blood Cells, Molecules, and Diseases. 2011 ; Vol. 46, No. 3. pp. 230-234.
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AU - Maia, Raquel C.

AU - Vasconcelos, Flávia C.

AU - Abdelhay, Eliana

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AU - Ferreira, Aline F.

AU - Castro, Fabíola A.

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