Increased expression of protease-activated receptor 1 (PAR-1) in human leukemias

Camilla de S.B. Veiga, Tatiana C. Carneiro-Lobo, Cláudia J.B.P. Coelho, Silvia M.F. Carvalho, Raquel C. Maia, Flávia C. Vasconcelos, Eliana Abdelhay, André L. Mencalha, Aline F. Ferreira, Fabíola A. Castro, Robson Q. Monteiro

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Abstract

Protease-activated receptor 1 (PAR-1) is a G-protein-coupled receptor that is overexpressed in solid tumors, being associated with several pro-tumoral responses including primary growth, invasion, metastasis and angiogenesis. Expression of PAR-1 in human leukemic cell lines is reported but the status of its expression in human leukemic patients is currently unknown. In this study we evaluated the expression pattern of PAR-1 in patients with the four main types of leukemia - chronic lymphocytic leukemia subtype B (B-CLL), acute lymphoblastic leukemia subtype B (B-ALL), acute myeloid leukemia (AML) and chronic myeloid leukemia (CML). Flow cytometry analyses show that lymphocytes from B-CLL patients express this receptor at similar levels to healthy individuals. On the other hand, it was observed a significant increase in PAR-1 expression in B-ALL lymphocytes as compared to B-CLL and healthy donors. Flow cytometric and real-time PCR demonstrated a significant increase in PAR-1 expression in granulocytes from CML patients in blast phase (CML-BP) but not in chronic phase (CML-CP) as compared to healthy donors. Finally, a significant increase in PAR-1 expression has been also observed in blasts from AML (subtypes M4 and M5) patients, as compared to monocytes or granulocytes from healthy donors. We conclude that PAR-1 might play an important biological role in aggressive leukemias and might offer additional strategies for the development of new therapies.

Original languageEnglish
Pages (from-to)230-234
Number of pages5
JournalBlood Cells, Molecules, and Diseases
Volume46
Issue number3
DOIs
Publication statusPublished - 15 Mar 2011

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Keywords

  • Acute myeloid leukemia
  • Chronic myeloid leukemia
  • Protease-activated receptor 1 (PAR-1)
  • Thrombin

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Hematology
  • Cell Biology

Cite this

Veiga, C. D. S. B., Carneiro-Lobo, T. C., Coelho, C. J. B. P., Carvalho, S. M. F., Maia, R. C., Vasconcelos, F. C., Abdelhay, E., Mencalha, A. L., Ferreira, A. F., Castro, F. A., & Monteiro, R. Q. (2011). Increased expression of protease-activated receptor 1 (PAR-1) in human leukemias. Blood Cells, Molecules, and Diseases, 46(3), 230-234. https://doi.org/10.1016/j.bcmd.2010.12.005