In vivo studies fail to reveal a role for IL-4 or STAT6 signaling in Th2 lymphocyte differentiation

Nicholas J. Van Panhuys, Shiau Choot Tang, Melanie Prout, Mali Camberis, Debbie Scarlett, Joanna Roberts, Jane Hu-Li, William E. Paul, Graham Le Gros

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

The expression of interleukin-4 (IL-4) is viewed as the hallmark of a Th2 lymphocyte, whereas the subsequent action of IL-4 and IL-13, mediated through the STAT6 signaling pathway, is seen as a prerequisite for the full development of Th2 immune responses to parasites and allergens. G4 mice, whose IL-4 gene locus contains the fluorescent reporter eGFP, were used to quantify the number of Th2 cells that develop during Nippostrongylus brasiliensis- or allergen-induced immune responses under conditions where IL-4 or STAT6 was absent. Here, we show that deletion of IL-4 or STAT6 had little impact on the number or timing of appearance of IL-4-producing Th2 cells. These data indicate that in vivo differentiation of naïve CD4 T cells to Th2 status often occurs independently of IL-4 and STAT6 and that recently described pathways of Th2 cell differentiation may explain how allergens and parasites selectively induce Th2-mediated immunity.

Original languageEnglish
Pages (from-to)12423-12428
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number34
DOIs
Publication statusPublished - 26 Aug 2008
Externally publishedYes

Fingerprint

Interleukin-4
Lymphocytes
Th2 Cells
Allergens
Parasites
Nippostrongylus
Interleukin-13
Cell Differentiation
Immunity
T-Lymphocytes
Genes

Keywords

  • Allergen
  • Asthma
  • Cytokine
  • Nippostrongylus

ASJC Scopus subject areas

  • General

Cite this

In vivo studies fail to reveal a role for IL-4 or STAT6 signaling in Th2 lymphocyte differentiation. / Van Panhuys, Nicholas J.; Tang, Shiau Choot; Prout, Melanie; Camberis, Mali; Scarlett, Debbie; Roberts, Joanna; Hu-Li, Jane; Paul, William E.; Le Gros, Graham.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 34, 26.08.2008, p. 12423-12428.

Research output: Contribution to journalArticle

Van Panhuys, Nicholas J. ; Tang, Shiau Choot ; Prout, Melanie ; Camberis, Mali ; Scarlett, Debbie ; Roberts, Joanna ; Hu-Li, Jane ; Paul, William E. ; Le Gros, Graham. / In vivo studies fail to reveal a role for IL-4 or STAT6 signaling in Th2 lymphocyte differentiation. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 34. pp. 12423-12428.
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AU - Scarlett, Debbie

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