In vitro QuantiFERON-TB gold antigen specific interleukin-1beta to diagnose TB among HIV-positive subjects

Maddineni Prabhavathi, Basirudeen S. Kabeer, Anbarasu Deenadayalan, Alamelu Raja

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background The recently introduced IFN-γ release assay (IGRA) has been reported to improve the diagnosis of TB. However, IGRA has suboptimal sensitivity to diagnose TB among HIV co-infected subjects. Apart from IFN-γ, the pro inflammatory cytokines such as Interleukin-1beta (IL-1β), Tumor necrosis factor-alpha (TNF-α), IL-2, IL-6, IL-8 and IL-12 are also play a major role in mycobacterial infections. This study aimed to analyze these cytokines for detecting active TB among HIV sero positive subjects. Materials and methods We had prospectively enrolled 53 HIV positive subjects and 55 HIV-TB co-infected patients from India. IGRA was performed by using QuantiFERON TB-Gold In tube (QFT-GIT) method. TB antigen specific IL-1β, TNF-α, IL-2, IL-6, IL-8 and IL-12 levels were evaluated by ELISA in plasma harvested from QFT-GIT tubes. Results and conclusion The TB antigen specific IL-1β levels were significantly elevated in HIV-TB co-infected patients compared to HIV positive subjects (p = 0.0004). The specificity of both IL-1β (50.94%) and QFT-GIT (52.83%) remained similar in HIV positive subjects (p = 0.24). However, IL-1β had shown higher sensitivity (72.73%) than QFT-GIT (54.55%) to diagnose TB among HIV co-infected patients. Moreover, in culture test positive HIV-TB patients, antigen specific IL-1β exhibited sensitivity of 84.21%; whereas QFT-GIT exhibited only 57.89% sensitivity. Unlike IFN-γ (the read out marker of QFT-GIT), antigen specific IL-1β levels were not influenced by low CD4 counts. The other cytokine levels were not significantly differ between the 2 groups.From this study we concluded that TB antigen specific IL-1β may be an additional biomarker for active TB diagnosis among HIV positive subjects.

Original languageEnglish
Pages (from-to)27-30
Number of pages4
JournalTuberculosis
Volume96
DOIs
Publication statusPublished - 1 Jan 2016

Fingerprint

Interleukin-1beta
Gold
HIV
Antigens
Interleukin-12
Cytokines
Interleukin-8
Interleukin-2
Interleukin-6
Tumor Necrosis Factor-alpha
In Vitro Techniques
CD4 Lymphocyte Count
India
Biomarkers
Enzyme-Linked Immunosorbent Assay
Infection

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

In vitro QuantiFERON-TB gold antigen specific interleukin-1beta to diagnose TB among HIV-positive subjects. / Prabhavathi, Maddineni; Kabeer, Basirudeen S.; Deenadayalan, Anbarasu; Raja, Alamelu.

In: Tuberculosis, Vol. 96, 01.01.2016, p. 27-30.

Research output: Contribution to journalArticle

Prabhavathi, Maddineni ; Kabeer, Basirudeen S. ; Deenadayalan, Anbarasu ; Raja, Alamelu. / In vitro QuantiFERON-TB gold antigen specific interleukin-1beta to diagnose TB among HIV-positive subjects. In: Tuberculosis. 2016 ; Vol. 96. pp. 27-30.
@article{54bc71e242794c879fc67689f3cb7595,
title = "In vitro QuantiFERON-TB gold antigen specific interleukin-1beta to diagnose TB among HIV-positive subjects",
abstract = "Background The recently introduced IFN-γ release assay (IGRA) has been reported to improve the diagnosis of TB. However, IGRA has suboptimal sensitivity to diagnose TB among HIV co-infected subjects. Apart from IFN-γ, the pro inflammatory cytokines such as Interleukin-1beta (IL-1β), Tumor necrosis factor-alpha (TNF-α), IL-2, IL-6, IL-8 and IL-12 are also play a major role in mycobacterial infections. This study aimed to analyze these cytokines for detecting active TB among HIV sero positive subjects. Materials and methods We had prospectively enrolled 53 HIV positive subjects and 55 HIV-TB co-infected patients from India. IGRA was performed by using QuantiFERON TB-Gold In tube (QFT-GIT) method. TB antigen specific IL-1β, TNF-α, IL-2, IL-6, IL-8 and IL-12 levels were evaluated by ELISA in plasma harvested from QFT-GIT tubes. Results and conclusion The TB antigen specific IL-1β levels were significantly elevated in HIV-TB co-infected patients compared to HIV positive subjects (p = 0.0004). The specificity of both IL-1β (50.94{\%}) and QFT-GIT (52.83{\%}) remained similar in HIV positive subjects (p = 0.24). However, IL-1β had shown higher sensitivity (72.73{\%}) than QFT-GIT (54.55{\%}) to diagnose TB among HIV co-infected patients. Moreover, in culture test positive HIV-TB patients, antigen specific IL-1β exhibited sensitivity of 84.21{\%}; whereas QFT-GIT exhibited only 57.89{\%} sensitivity. Unlike IFN-γ (the read out marker of QFT-GIT), antigen specific IL-1β levels were not influenced by low CD4 counts. The other cytokine levels were not significantly differ between the 2 groups.From this study we concluded that TB antigen specific IL-1β may be an additional biomarker for active TB diagnosis among HIV positive subjects.",
author = "Maddineni Prabhavathi and Kabeer, {Basirudeen S.} and Anbarasu Deenadayalan and Alamelu Raja",
year = "2016",
month = "1",
day = "1",
doi = "10.1016/j.tube.2015.10.005",
language = "English",
volume = "96",
pages = "27--30",
journal = "Tuberculosis",
issn = "1472-9792",
publisher = "Churchill Livingstone",

}

TY - JOUR

T1 - In vitro QuantiFERON-TB gold antigen specific interleukin-1beta to diagnose TB among HIV-positive subjects

AU - Prabhavathi, Maddineni

AU - Kabeer, Basirudeen S.

AU - Deenadayalan, Anbarasu

AU - Raja, Alamelu

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Background The recently introduced IFN-γ release assay (IGRA) has been reported to improve the diagnosis of TB. However, IGRA has suboptimal sensitivity to diagnose TB among HIV co-infected subjects. Apart from IFN-γ, the pro inflammatory cytokines such as Interleukin-1beta (IL-1β), Tumor necrosis factor-alpha (TNF-α), IL-2, IL-6, IL-8 and IL-12 are also play a major role in mycobacterial infections. This study aimed to analyze these cytokines for detecting active TB among HIV sero positive subjects. Materials and methods We had prospectively enrolled 53 HIV positive subjects and 55 HIV-TB co-infected patients from India. IGRA was performed by using QuantiFERON TB-Gold In tube (QFT-GIT) method. TB antigen specific IL-1β, TNF-α, IL-2, IL-6, IL-8 and IL-12 levels were evaluated by ELISA in plasma harvested from QFT-GIT tubes. Results and conclusion The TB antigen specific IL-1β levels were significantly elevated in HIV-TB co-infected patients compared to HIV positive subjects (p = 0.0004). The specificity of both IL-1β (50.94%) and QFT-GIT (52.83%) remained similar in HIV positive subjects (p = 0.24). However, IL-1β had shown higher sensitivity (72.73%) than QFT-GIT (54.55%) to diagnose TB among HIV co-infected patients. Moreover, in culture test positive HIV-TB patients, antigen specific IL-1β exhibited sensitivity of 84.21%; whereas QFT-GIT exhibited only 57.89% sensitivity. Unlike IFN-γ (the read out marker of QFT-GIT), antigen specific IL-1β levels were not influenced by low CD4 counts. The other cytokine levels were not significantly differ between the 2 groups.From this study we concluded that TB antigen specific IL-1β may be an additional biomarker for active TB diagnosis among HIV positive subjects.

AB - Background The recently introduced IFN-γ release assay (IGRA) has been reported to improve the diagnosis of TB. However, IGRA has suboptimal sensitivity to diagnose TB among HIV co-infected subjects. Apart from IFN-γ, the pro inflammatory cytokines such as Interleukin-1beta (IL-1β), Tumor necrosis factor-alpha (TNF-α), IL-2, IL-6, IL-8 and IL-12 are also play a major role in mycobacterial infections. This study aimed to analyze these cytokines for detecting active TB among HIV sero positive subjects. Materials and methods We had prospectively enrolled 53 HIV positive subjects and 55 HIV-TB co-infected patients from India. IGRA was performed by using QuantiFERON TB-Gold In tube (QFT-GIT) method. TB antigen specific IL-1β, TNF-α, IL-2, IL-6, IL-8 and IL-12 levels were evaluated by ELISA in plasma harvested from QFT-GIT tubes. Results and conclusion The TB antigen specific IL-1β levels were significantly elevated in HIV-TB co-infected patients compared to HIV positive subjects (p = 0.0004). The specificity of both IL-1β (50.94%) and QFT-GIT (52.83%) remained similar in HIV positive subjects (p = 0.24). However, IL-1β had shown higher sensitivity (72.73%) than QFT-GIT (54.55%) to diagnose TB among HIV co-infected patients. Moreover, in culture test positive HIV-TB patients, antigen specific IL-1β exhibited sensitivity of 84.21%; whereas QFT-GIT exhibited only 57.89% sensitivity. Unlike IFN-γ (the read out marker of QFT-GIT), antigen specific IL-1β levels were not influenced by low CD4 counts. The other cytokine levels were not significantly differ between the 2 groups.From this study we concluded that TB antigen specific IL-1β may be an additional biomarker for active TB diagnosis among HIV positive subjects.

UR - http://www.scopus.com/inward/record.url?scp=84954448966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84954448966&partnerID=8YFLogxK

U2 - 10.1016/j.tube.2015.10.005

DO - 10.1016/j.tube.2015.10.005

M3 - Article

C2 - 26786651

AN - SCOPUS:84954448966

VL - 96

SP - 27

EP - 30

JO - Tuberculosis

JF - Tuberculosis

SN - 1472-9792

ER -