In situ optical mapping of voltage and calcium in the heart

Peter Lee, Fouad Taghavi, Ping Yan, Paul Ewart, Euan A. Ashley, Leslie M. Loew, Peter Kohl, Christian Bollensdorff, Christopher E. Woods

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Electroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological function with fast and simultaneous data acquisition. If applied to the clinical setting, this could improve both diagnostic potential and therapeutic efficacy of clinical arrhythmia interventions. The aim of this study was to explore this utility in vivo using a rat model. To this aim, we present a single-camera imaging and multiple light-emitting-diode illumination system that reduces economic and technical implementation hurdles to cardiac optical mapping. Combined with a red-shifted calcium dye and a new near-infrared voltage-sensitive dye, both suitable for use in blood-perfused tissue, we demonstrate the feasibility of in vivo multi-parametric imaging of the mammalian heart. Our approach combines recording of electrophysiologically-relevant parameters with observation of structural substrates and is adaptable, in principle, to trans-catheter percutaneous approaches.

Original languageEnglish
Article numbere42562
JournalPLoS One
Volume7
Issue number8
DOIs
Publication statusPublished - 2 Aug 2012
Externally publishedYes

Fingerprint

arrhythmia
dyes
Cardiac Arrhythmias
Coloring Agents
heart
Cardiac Electrophysiology
image analysis
Calcium
calcium
economic systems
electrophysiology
Electric potential
Lighting
catheters
cameras
Imaging techniques
lighting
Anatomy
Catheters
animal models

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Lee, P., Taghavi, F., Yan, P., Ewart, P., Ashley, E. A., Loew, L. M., ... Woods, C. E. (2012). In situ optical mapping of voltage and calcium in the heart. PLoS One, 7(8), [e42562]. https://doi.org/10.1371/journal.pone.0042562

In situ optical mapping of voltage and calcium in the heart. / Lee, Peter; Taghavi, Fouad; Yan, Ping; Ewart, Paul; Ashley, Euan A.; Loew, Leslie M.; Kohl, Peter; Bollensdorff, Christian; Woods, Christopher E.

In: PLoS One, Vol. 7, No. 8, e42562, 02.08.2012.

Research output: Contribution to journalArticle

Lee, P, Taghavi, F, Yan, P, Ewart, P, Ashley, EA, Loew, LM, Kohl, P, Bollensdorff, C & Woods, CE 2012, 'In situ optical mapping of voltage and calcium in the heart', PLoS One, vol. 7, no. 8, e42562. https://doi.org/10.1371/journal.pone.0042562
Lee P, Taghavi F, Yan P, Ewart P, Ashley EA, Loew LM et al. In situ optical mapping of voltage and calcium in the heart. PLoS One. 2012 Aug 2;7(8). e42562. https://doi.org/10.1371/journal.pone.0042562
Lee, Peter ; Taghavi, Fouad ; Yan, Ping ; Ewart, Paul ; Ashley, Euan A. ; Loew, Leslie M. ; Kohl, Peter ; Bollensdorff, Christian ; Woods, Christopher E. / In situ optical mapping of voltage and calcium in the heart. In: PLoS One. 2012 ; Vol. 7, No. 8.
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