In situ optical mapping of voltage and calcium in the heart

Peter Lee, Fouad Taghavi, Ping Yan, Paul Ewart, Euan A. Ashley, Leslie M. Loew, Peter Kohl, Christian Bollensdorff, Christopher E. Woods

    Research output: Contribution to journalArticle

    18 Citations (Scopus)


    Electroanatomic mapping the interrelation of intracardiac electrical activation with anatomic locations has become an important tool for clinical assessment of complex arrhythmias. Optical mapping of cardiac electrophysiology combines high spatiotemporal resolution of anatomy and physiological function with fast and simultaneous data acquisition. If applied to the clinical setting, this could improve both diagnostic potential and therapeutic efficacy of clinical arrhythmia interventions. The aim of this study was to explore this utility in vivo using a rat model. To this aim, we present a single-camera imaging and multiple light-emitting-diode illumination system that reduces economic and technical implementation hurdles to cardiac optical mapping. Combined with a red-shifted calcium dye and a new near-infrared voltage-sensitive dye, both suitable for use in blood-perfused tissue, we demonstrate the feasibility of in vivo multi-parametric imaging of the mammalian heart. Our approach combines recording of electrophysiologically-relevant parameters with observation of structural substrates and is adaptable, in principle, to trans-catheter percutaneous approaches.

    Original languageEnglish
    Article numbere42562
    JournalPloS one
    Issue number8
    Publication statusPublished - 2 Aug 2012


    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)
    • General

    Cite this

    Lee, P., Taghavi, F., Yan, P., Ewart, P., Ashley, E. A., Loew, L. M., Kohl, P., Bollensdorff, C., & Woods, C. E. (2012). In situ optical mapping of voltage and calcium in the heart. PloS one, 7(8), [e42562].