Improved physiology and metabolic flux after Roux-en-Y gastric bypass is associated with temporal changes in the circulating microRNAome: A longitudinal study in humans

Abdullah Alkandari, Hutan Ashrafian, Thozhukat Sathyapalan, Peter Sedman, Ara Darzi, Elaine Holmes, Thanos Athanasiou, Stephen Atkin, Nigel J. Gooderham

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: The global pandemic of obesity and the metabolic syndrome are leading causes of mortality and morbidity. Bariatric surgery leads to sustained weight loss and improves obesity-associated morbidity including remission of type 2 diabetes. MicroRNAs are small, endogenous RNAs that regulate gene expression post-transcriptionally, controlling most of the human transcriptome and contributing to the regulation of systemic metabolism. This preliminary, longitudinal, repeat sampling study, in which subjects acted as their own control, aimed to assess the temporal effect of bariatric surgery on circulating microRNA expression profiles. Methods: We used Exiqon's optimized circulating microRNA panel (comprising 179 validated miRNAs) and miRCURY locked nucleic acid plasma/serum Polymerase Chain Reaction (PCR) to assess circulating microRNA expression. The microRNAome was determined for Roux-en-Y gastric bypass (RYGB) patients examined preoperatively and at 1 month, 3 months, 6 months, 9 months and 12 months postoperatively. Data was analysed using multivariate and univariate statistics. Results: Compared to the preoperative circulating microRNA expression profile, RYGB altered the circulating microRNAome in a time dependent manner and the expression of 48 circulating microRNAs were significantly different. Importantly, these latter microRNAs are associated with pathways involved in regulation and rescue from metabolic dysfunction and correlated with BMI, the percentage of excess weight loss and fasting blood glucose levels. Conclusions: The results of this pilot study show that RYGB fundamentally alters microRNA expression in circulation with a time-dependent progressive departure in profile from the preoperative baseline and indicate that microRNAs are potentially novel biomarkers for the benefits of bariatric surgery.

Original languageEnglish
Article number20
JournalBMC Obesity
Volume5
Issue number1
DOIs
Publication statusPublished - 31 May 2018

Fingerprint

Gastric Bypass
MicroRNAs
Longitudinal Studies
Bariatric Surgery
Weight Loss
Obesity
Morbidity
Sampling Studies
Pandemics
Transcriptome
Type 2 Diabetes Mellitus
Blood Glucose
Fasting
Biomarkers
RNA
Gene Expression
Polymerase Chain Reaction

Keywords

  • Bariatric
  • Biomarker
  • Circulating
  • Gastric bypass
  • Longitudinal
  • MicroRNA
  • MiRNA
  • RYGB
  • Temporal

ASJC Scopus subject areas

  • Physical Therapy, Sports Therapy and Rehabilitation
  • Epidemiology
  • Health Policy
  • Public Health, Environmental and Occupational Health
  • Endocrinology, Diabetes and Metabolism

Cite this

Improved physiology and metabolic flux after Roux-en-Y gastric bypass is associated with temporal changes in the circulating microRNAome : A longitudinal study in humans. / Alkandari, Abdullah; Ashrafian, Hutan; Sathyapalan, Thozhukat; Sedman, Peter; Darzi, Ara; Holmes, Elaine; Athanasiou, Thanos; Atkin, Stephen; Gooderham, Nigel J.

In: BMC Obesity, Vol. 5, No. 1, 20, 31.05.2018.

Research output: Contribution to journalArticle

Alkandari, Abdullah ; Ashrafian, Hutan ; Sathyapalan, Thozhukat ; Sedman, Peter ; Darzi, Ara ; Holmes, Elaine ; Athanasiou, Thanos ; Atkin, Stephen ; Gooderham, Nigel J. / Improved physiology and metabolic flux after Roux-en-Y gastric bypass is associated with temporal changes in the circulating microRNAome : A longitudinal study in humans. In: BMC Obesity. 2018 ; Vol. 5, No. 1.
@article{8a1c7cdf789e4744b1132bd569ecfca8,
title = "Improved physiology and metabolic flux after Roux-en-Y gastric bypass is associated with temporal changes in the circulating microRNAome: A longitudinal study in humans",
abstract = "Background: The global pandemic of obesity and the metabolic syndrome are leading causes of mortality and morbidity. Bariatric surgery leads to sustained weight loss and improves obesity-associated morbidity including remission of type 2 diabetes. MicroRNAs are small, endogenous RNAs that regulate gene expression post-transcriptionally, controlling most of the human transcriptome and contributing to the regulation of systemic metabolism. This preliminary, longitudinal, repeat sampling study, in which subjects acted as their own control, aimed to assess the temporal effect of bariatric surgery on circulating microRNA expression profiles. Methods: We used Exiqon's optimized circulating microRNA panel (comprising 179 validated miRNAs) and miRCURY locked nucleic acid plasma/serum Polymerase Chain Reaction (PCR) to assess circulating microRNA expression. The microRNAome was determined for Roux-en-Y gastric bypass (RYGB) patients examined preoperatively and at 1 month, 3 months, 6 months, 9 months and 12 months postoperatively. Data was analysed using multivariate and univariate statistics. Results: Compared to the preoperative circulating microRNA expression profile, RYGB altered the circulating microRNAome in a time dependent manner and the expression of 48 circulating microRNAs were significantly different. Importantly, these latter microRNAs are associated with pathways involved in regulation and rescue from metabolic dysfunction and correlated with BMI, the percentage of excess weight loss and fasting blood glucose levels. Conclusions: The results of this pilot study show that RYGB fundamentally alters microRNA expression in circulation with a time-dependent progressive departure in profile from the preoperative baseline and indicate that microRNAs are potentially novel biomarkers for the benefits of bariatric surgery.",
keywords = "Bariatric, Biomarker, Circulating, Gastric bypass, Longitudinal, MicroRNA, MiRNA, RYGB, Temporal",
author = "Abdullah Alkandari and Hutan Ashrafian and Thozhukat Sathyapalan and Peter Sedman and Ara Darzi and Elaine Holmes and Thanos Athanasiou and Stephen Atkin and Gooderham, {Nigel J.}",
year = "2018",
month = "5",
day = "31",
doi = "10.1186/s40608-018-0199-z",
language = "English",
volume = "5",
journal = "BMC Obesity",
issn = "2052-9538",
publisher = "BioMed Central Ltd.",
number = "1",

}

TY - JOUR

T1 - Improved physiology and metabolic flux after Roux-en-Y gastric bypass is associated with temporal changes in the circulating microRNAome

T2 - A longitudinal study in humans

AU - Alkandari, Abdullah

AU - Ashrafian, Hutan

AU - Sathyapalan, Thozhukat

AU - Sedman, Peter

AU - Darzi, Ara

AU - Holmes, Elaine

AU - Athanasiou, Thanos

AU - Atkin, Stephen

AU - Gooderham, Nigel J.

PY - 2018/5/31

Y1 - 2018/5/31

N2 - Background: The global pandemic of obesity and the metabolic syndrome are leading causes of mortality and morbidity. Bariatric surgery leads to sustained weight loss and improves obesity-associated morbidity including remission of type 2 diabetes. MicroRNAs are small, endogenous RNAs that regulate gene expression post-transcriptionally, controlling most of the human transcriptome and contributing to the regulation of systemic metabolism. This preliminary, longitudinal, repeat sampling study, in which subjects acted as their own control, aimed to assess the temporal effect of bariatric surgery on circulating microRNA expression profiles. Methods: We used Exiqon's optimized circulating microRNA panel (comprising 179 validated miRNAs) and miRCURY locked nucleic acid plasma/serum Polymerase Chain Reaction (PCR) to assess circulating microRNA expression. The microRNAome was determined for Roux-en-Y gastric bypass (RYGB) patients examined preoperatively and at 1 month, 3 months, 6 months, 9 months and 12 months postoperatively. Data was analysed using multivariate and univariate statistics. Results: Compared to the preoperative circulating microRNA expression profile, RYGB altered the circulating microRNAome in a time dependent manner and the expression of 48 circulating microRNAs were significantly different. Importantly, these latter microRNAs are associated with pathways involved in regulation and rescue from metabolic dysfunction and correlated with BMI, the percentage of excess weight loss and fasting blood glucose levels. Conclusions: The results of this pilot study show that RYGB fundamentally alters microRNA expression in circulation with a time-dependent progressive departure in profile from the preoperative baseline and indicate that microRNAs are potentially novel biomarkers for the benefits of bariatric surgery.

AB - Background: The global pandemic of obesity and the metabolic syndrome are leading causes of mortality and morbidity. Bariatric surgery leads to sustained weight loss and improves obesity-associated morbidity including remission of type 2 diabetes. MicroRNAs are small, endogenous RNAs that regulate gene expression post-transcriptionally, controlling most of the human transcriptome and contributing to the regulation of systemic metabolism. This preliminary, longitudinal, repeat sampling study, in which subjects acted as their own control, aimed to assess the temporal effect of bariatric surgery on circulating microRNA expression profiles. Methods: We used Exiqon's optimized circulating microRNA panel (comprising 179 validated miRNAs) and miRCURY locked nucleic acid plasma/serum Polymerase Chain Reaction (PCR) to assess circulating microRNA expression. The microRNAome was determined for Roux-en-Y gastric bypass (RYGB) patients examined preoperatively and at 1 month, 3 months, 6 months, 9 months and 12 months postoperatively. Data was analysed using multivariate and univariate statistics. Results: Compared to the preoperative circulating microRNA expression profile, RYGB altered the circulating microRNAome in a time dependent manner and the expression of 48 circulating microRNAs were significantly different. Importantly, these latter microRNAs are associated with pathways involved in regulation and rescue from metabolic dysfunction and correlated with BMI, the percentage of excess weight loss and fasting blood glucose levels. Conclusions: The results of this pilot study show that RYGB fundamentally alters microRNA expression in circulation with a time-dependent progressive departure in profile from the preoperative baseline and indicate that microRNAs are potentially novel biomarkers for the benefits of bariatric surgery.

KW - Bariatric

KW - Biomarker

KW - Circulating

KW - Gastric bypass

KW - Longitudinal

KW - MicroRNA

KW - MiRNA

KW - RYGB

KW - Temporal

UR - http://www.scopus.com/inward/record.url?scp=85047893948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047893948&partnerID=8YFLogxK

U2 - 10.1186/s40608-018-0199-z

DO - 10.1186/s40608-018-0199-z

M3 - Article

AN - SCOPUS:85047893948

VL - 5

JO - BMC Obesity

JF - BMC Obesity

SN - 2052-9538

IS - 1

M1 - 20

ER -