Improved diagnosis of tuberculosis in HIV-positive patients using RD1-encoded antigen CFP-10

Parasa V.Ramana Rao, Madhan Kumar Murthy, S. Basirudeen, Pawan Sharma, Soumya Swaminathan, Alamelu Raja

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: The present study was aimed at determining the serodiagnostic potential of 38-kDa (Rv0934, Mycobacterium tuberculosis complex-specific antigen) and CFP-10 (Rv3874, RD1 antigen) antigens among HIV-positive and HIV-negative patients with pulmonary TB. Methods: The diagnostic potential of native 38-kDa (n38-kDa) and recombinant CFP-10 (rCFP-10) antigens was ascertained in terms of sensitivity and specificity using an indirect ELISA. The study included 508 HIV-seronegative TB patients (TB), 54 HIV-seropositive TB patients (HIV-TB), 30 HIV-positive patients without TB (HIV), and 256 controls. Results: In HIV-TB, the sensitivities for individual antigens ranged from 14.8% to 31.5% and the specificity was >98% for IgG. When IgA results were added to IgG, the sensitivity increased to 25.9% for 38-kDa and 57.4% for CFP-10; specificity changed to 97.5% for 38-kDa and 98.1% for CFP-10. The combined results of both the antigens gave 59.3% sensitivity and 95.6% specificity. In TB, the sensitivity was 82.8% when the antigen results were combined. None of the HIV-infected controls showed positivity for IgG to either of the two antigens. Conclusion: Use of CFP-10 enhances the sensitivity of 38-kDa, and therefore the 38-kDa and CFP-10 antigen combination can be a diagnostic marker in HIV-TB.

Original languageEnglish
Pages (from-to)613-622
Number of pages10
JournalInternational Journal of Infectious Diseases
Volume13
Issue number5
DOIs
Publication statusPublished - 1 Sep 2009

    Fingerprint

Keywords

  • CFP-10
  • ELISA
  • HIV-TB
  • RD1
  • Serodiagnosis
  • Tuberculosis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this