Improved diagnosis of tuberculosis in HIV-positive patients using RD1-encoded antigen CFP-10

Parasa V Ramana Rao, Madhan Kumar Murthy, Basirudeen S. Kabeer, Pawan Sharma, Soumya Swaminathan, Alamelu Raja

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: The present study was aimed at determining the serodiagnostic potential of 38-kDa (Rv0934, Mycobacterium tuberculosis complex-specific antigen) and CFP-10 (Rv3874, RD1 antigen) antigens among HIV-positive and HIV-negative patients with pulmonary TB. Methods: The diagnostic potential of native 38-kDa (n38-kDa) and recombinant CFP-10 (rCFP-10) antigens was ascertained in terms of sensitivity and specificity using an indirect ELISA. The study included 508 HIV-seronegative TB patients (TB), 54 HIV-seropositive TB patients (HIV-TB), 30 HIV-positive patients without TB (HIV), and 256 controls. Results: In HIV-TB, the sensitivities for individual antigens ranged from 14.8% to 31.5% and the specificity was >98% for IgG. When IgA results were added to IgG, the sensitivity increased to 25.9% for 38-kDa and 57.4% for CFP-10; specificity changed to 97.5% for 38-kDa and 98.1% for CFP-10. The combined results of both the antigens gave 59.3% sensitivity and 95.6% specificity. In TB, the sensitivity was 82.8% when the antigen results were combined. None of the HIV-infected controls showed positivity for IgG to either of the two antigens. Conclusion: Use of CFP-10 enhances the sensitivity of 38-kDa, and therefore the 38-kDa and CFP-10 antigen combination can be a diagnostic marker in HIV-TB.

Original languageEnglish
Pages (from-to)613-622
Number of pages10
JournalInternational Journal of Infectious Diseases
Volume13
Issue number5
DOIs
Publication statusPublished - Sep 2009
Externally publishedYes

Fingerprint

Tuberculosis
HIV
Antigens
Immunoglobulin G
HIV Antigens
Sensitivity and Specificity
Mycobacterium tuberculosis
Immunoglobulin A
Enzyme-Linked Immunosorbent Assay
Lung

Keywords

  • CFP-10
  • ELISA
  • HIV-TB
  • RD1
  • Serodiagnosis
  • Tuberculosis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Improved diagnosis of tuberculosis in HIV-positive patients using RD1-encoded antigen CFP-10. / Rao, Parasa V Ramana; Murthy, Madhan Kumar; Kabeer, Basirudeen S.; Sharma, Pawan; Swaminathan, Soumya; Raja, Alamelu.

In: International Journal of Infectious Diseases, Vol. 13, No. 5, 09.2009, p. 613-622.

Research output: Contribution to journalArticle

Rao, Parasa V Ramana ; Murthy, Madhan Kumar ; Kabeer, Basirudeen S. ; Sharma, Pawan ; Swaminathan, Soumya ; Raja, Alamelu. / Improved diagnosis of tuberculosis in HIV-positive patients using RD1-encoded antigen CFP-10. In: International Journal of Infectious Diseases. 2009 ; Vol. 13, No. 5. pp. 613-622.
@article{f9e47e37c8df4eae84ebb8a5de1e2a08,
title = "Improved diagnosis of tuberculosis in HIV-positive patients using RD1-encoded antigen CFP-10",
abstract = "Objective: The present study was aimed at determining the serodiagnostic potential of 38-kDa (Rv0934, Mycobacterium tuberculosis complex-specific antigen) and CFP-10 (Rv3874, RD1 antigen) antigens among HIV-positive and HIV-negative patients with pulmonary TB. Methods: The diagnostic potential of native 38-kDa (n38-kDa) and recombinant CFP-10 (rCFP-10) antigens was ascertained in terms of sensitivity and specificity using an indirect ELISA. The study included 508 HIV-seronegative TB patients (TB), 54 HIV-seropositive TB patients (HIV-TB), 30 HIV-positive patients without TB (HIV), and 256 controls. Results: In HIV-TB, the sensitivities for individual antigens ranged from 14.8{\%} to 31.5{\%} and the specificity was >98{\%} for IgG. When IgA results were added to IgG, the sensitivity increased to 25.9{\%} for 38-kDa and 57.4{\%} for CFP-10; specificity changed to 97.5{\%} for 38-kDa and 98.1{\%} for CFP-10. The combined results of both the antigens gave 59.3{\%} sensitivity and 95.6{\%} specificity. In TB, the sensitivity was 82.8{\%} when the antigen results were combined. None of the HIV-infected controls showed positivity for IgG to either of the two antigens. Conclusion: Use of CFP-10 enhances the sensitivity of 38-kDa, and therefore the 38-kDa and CFP-10 antigen combination can be a diagnostic marker in HIV-TB.",
keywords = "CFP-10, ELISA, HIV-TB, RD1, Serodiagnosis, Tuberculosis",
author = "Rao, {Parasa V Ramana} and Murthy, {Madhan Kumar} and Kabeer, {Basirudeen S.} and Pawan Sharma and Soumya Swaminathan and Alamelu Raja",
year = "2009",
month = "9",
doi = "10.1016/j.ijid.2008.09.022",
language = "English",
volume = "13",
pages = "613--622",
journal = "International Journal of Infectious Diseases",
issn = "1201-9712",
publisher = "Elsevier",
number = "5",

}

TY - JOUR

T1 - Improved diagnosis of tuberculosis in HIV-positive patients using RD1-encoded antigen CFP-10

AU - Rao, Parasa V Ramana

AU - Murthy, Madhan Kumar

AU - Kabeer, Basirudeen S.

AU - Sharma, Pawan

AU - Swaminathan, Soumya

AU - Raja, Alamelu

PY - 2009/9

Y1 - 2009/9

N2 - Objective: The present study was aimed at determining the serodiagnostic potential of 38-kDa (Rv0934, Mycobacterium tuberculosis complex-specific antigen) and CFP-10 (Rv3874, RD1 antigen) antigens among HIV-positive and HIV-negative patients with pulmonary TB. Methods: The diagnostic potential of native 38-kDa (n38-kDa) and recombinant CFP-10 (rCFP-10) antigens was ascertained in terms of sensitivity and specificity using an indirect ELISA. The study included 508 HIV-seronegative TB patients (TB), 54 HIV-seropositive TB patients (HIV-TB), 30 HIV-positive patients without TB (HIV), and 256 controls. Results: In HIV-TB, the sensitivities for individual antigens ranged from 14.8% to 31.5% and the specificity was >98% for IgG. When IgA results were added to IgG, the sensitivity increased to 25.9% for 38-kDa and 57.4% for CFP-10; specificity changed to 97.5% for 38-kDa and 98.1% for CFP-10. The combined results of both the antigens gave 59.3% sensitivity and 95.6% specificity. In TB, the sensitivity was 82.8% when the antigen results were combined. None of the HIV-infected controls showed positivity for IgG to either of the two antigens. Conclusion: Use of CFP-10 enhances the sensitivity of 38-kDa, and therefore the 38-kDa and CFP-10 antigen combination can be a diagnostic marker in HIV-TB.

AB - Objective: The present study was aimed at determining the serodiagnostic potential of 38-kDa (Rv0934, Mycobacterium tuberculosis complex-specific antigen) and CFP-10 (Rv3874, RD1 antigen) antigens among HIV-positive and HIV-negative patients with pulmonary TB. Methods: The diagnostic potential of native 38-kDa (n38-kDa) and recombinant CFP-10 (rCFP-10) antigens was ascertained in terms of sensitivity and specificity using an indirect ELISA. The study included 508 HIV-seronegative TB patients (TB), 54 HIV-seropositive TB patients (HIV-TB), 30 HIV-positive patients without TB (HIV), and 256 controls. Results: In HIV-TB, the sensitivities for individual antigens ranged from 14.8% to 31.5% and the specificity was >98% for IgG. When IgA results were added to IgG, the sensitivity increased to 25.9% for 38-kDa and 57.4% for CFP-10; specificity changed to 97.5% for 38-kDa and 98.1% for CFP-10. The combined results of both the antigens gave 59.3% sensitivity and 95.6% specificity. In TB, the sensitivity was 82.8% when the antigen results were combined. None of the HIV-infected controls showed positivity for IgG to either of the two antigens. Conclusion: Use of CFP-10 enhances the sensitivity of 38-kDa, and therefore the 38-kDa and CFP-10 antigen combination can be a diagnostic marker in HIV-TB.

KW - CFP-10

KW - ELISA

KW - HIV-TB

KW - RD1

KW - Serodiagnosis

KW - Tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=68949213956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68949213956&partnerID=8YFLogxK

U2 - 10.1016/j.ijid.2008.09.022

DO - 10.1016/j.ijid.2008.09.022

M3 - Article

VL - 13

SP - 613

EP - 622

JO - International Journal of Infectious Diseases

JF - International Journal of Infectious Diseases

SN - 1201-9712

IS - 5

ER -