Impaired liver size and compromised neurobehavioral activity are elicited by chitosan nanoparticles in the zebrafish embryo model

Haissam Abou-Saleh, Nadin Younes, Kashif Rasool, Manaf H. Younis, Rafael M. Prieto, Hadi M. Yassine, Khaled Mahmoud, Gianfranco Pintus, Gheyath K. Nasrallah

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The use of chitosan nanoparticles (ChNPs) in various biological and environmental applications is attracting great interest. However, potential side effects related to ChNP toxicity remain the major limitation hampering their wide application. For the first time, we investigate the potential organ-specific (cardiac, hepatic, and neuromuscular) toxicity of ChNPs (size 100–150 nm) using the zebrafish embryo model. Our data highlight the absence of both acute and teratogenic toxic effects of ChNPs (~100% survival rate) even at the higher concentration employed (200 mg/L). Although no single sign of cardiotoxicity was observed upon exposure to 200 mg/L of ChNPs, as judged by heartbeat rate, the corrected QT interval (QTc, which measures the time between the start of the Q wave and the end of the T wave in the heart's electrical cycle), maximum cardiac arrest, and ejection fraction assays, the same dosage elicited the impairment of both liver size (decreased liver size, but without steatosis and lipid yolk retention) and neurobehavioral activity (increased movement under different light conditions). Although the observed toxic effect failed to affect embryo survival, whether a prolonged ChNP treatment may induce other potentially harmful effects remains to be elucidated. By reporting new insights on their organ-specific toxicity, our results add novel and useful information into the available data concerning the in vivo effect of ChNPs.

Original languageEnglish
Article number122
JournalNanomaterials
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

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Chitosan
Liver
Nanoparticles
Toxicity
Poisons
Lipids
Assays

Keywords

  • Chitosan
  • Nanoparticles
  • Organs specific toxicity
  • Zebrafish

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

Cite this

Abou-Saleh, H., Younes, N., Rasool, K., Younis, M. H., Prieto, R. M., Yassine, H. M., ... Nasrallah, G. K. (2019). Impaired liver size and compromised neurobehavioral activity are elicited by chitosan nanoparticles in the zebrafish embryo model. Nanomaterials, 9(1), [122]. https://doi.org/10.3390/nano9010122

Impaired liver size and compromised neurobehavioral activity are elicited by chitosan nanoparticles in the zebrafish embryo model. / Abou-Saleh, Haissam; Younes, Nadin; Rasool, Kashif; Younis, Manaf H.; Prieto, Rafael M.; Yassine, Hadi M.; Mahmoud, Khaled; Pintus, Gianfranco; Nasrallah, Gheyath K.

In: Nanomaterials, Vol. 9, No. 1, 122, 01.01.2019.

Research output: Contribution to journalArticle

Abou-Saleh, H, Younes, N, Rasool, K, Younis, MH, Prieto, RM, Yassine, HM, Mahmoud, K, Pintus, G & Nasrallah, GK 2019, 'Impaired liver size and compromised neurobehavioral activity are elicited by chitosan nanoparticles in the zebrafish embryo model', Nanomaterials, vol. 9, no. 1, 122. https://doi.org/10.3390/nano9010122
Abou-Saleh, Haissam ; Younes, Nadin ; Rasool, Kashif ; Younis, Manaf H. ; Prieto, Rafael M. ; Yassine, Hadi M. ; Mahmoud, Khaled ; Pintus, Gianfranco ; Nasrallah, Gheyath K. / Impaired liver size and compromised neurobehavioral activity are elicited by chitosan nanoparticles in the zebrafish embryo model. In: Nanomaterials. 2019 ; Vol. 9, No. 1.
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