Immunological properties of subcellular rat lymphocyte preparations. Primary allogeneic stimulation in vitro by fractions containing Ia (RT1-B), but not RT1-A antigens

A. Kaldany, C. B. Carpenter, C. A. Shadur, K. George, A. P. Lundin IIIrd, Manikkam Suthanthiran, T. B. Strom

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Abstract

Rat thymocyte membrane fractions have been prepared which exhibit strain-specific primary mixed-lymphocyte reaction (MLR)-stimulating and Ia (RT1-B) antigenic properties. These preparations lack the antigenicity of classical, serologically-defined RT1-A (Ag-B) antigens, as defined by in vitro serologic assays. Furthermore, after immunization of allogeneic hosts, specific anti-Ia and MLR-blocking antibodies, but not anti-AgB, alloantibodies are elaborated. Thymidine suicide experiments show that the same clones respond to whole cells and the fragments made from those cells, and the response segregates appropriately in F 2 progeny as a major histocompatibility complex (RT1)-linked phenomenon. Hence, it is possible to generate Ia-related allogeneic helper signals in primary, as well as secondary, in vitro responses, using subcellular membrane fragments that have restricted expression of RT1-B-, but not RT1-A-, encoded antigens.

Original languageEnglish
Pages (from-to)910-924
Number of pages15
JournalJournal of Experimental Medicine
Volume151
Issue number4
Publication statusPublished - 25 Jul 1980
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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