Immunolocalization of aquaporin-5 expression in sweat gland cells from normal and anhidrotic horses

Douglas Bovell, Susan L. Lindsay, Alistair D. Corbett, Catherine Steel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Western blot analysis showed that sweat gland cells from freely sweating horses expressed the water channel aquaporin-5 (AQP-5). Immunohistochemistry revealed a strong AQP-5-like activity reaction at the apical membrane of the glandular secretory cells, which was absent from the surrounding myoepithelium and all other skin structures. In anhidrotic horses, AQP-5 was also found at the apical membrane of the luminal sweat gland cells, but the level of expression reduced with the length of time that the animal had displayed anhidrosis. The level of AQP-5 expression was substantially reduced in animals with long-term anhidrosis, hence implicating water channel impairment as a possible factor in the development of this disorder.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalVeterinary Dermatology
Volume17
Issue number1
DOIs
Publication statusPublished - Feb 2006
Externally publishedYes

Fingerprint

Aquaporin 5
sweat glands
Sweat Glands
aquaporins
Horses
Hypohidrosis
horses
Aquaporins
cells
sweating
Sweating
Membranes
skin (animal)
immunohistochemistry
animals
Western blotting
water
Western Blotting
Immunohistochemistry
Skin

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Immunolocalization of aquaporin-5 expression in sweat gland cells from normal and anhidrotic horses. / Bovell, Douglas; Lindsay, Susan L.; Corbett, Alistair D.; Steel, Catherine.

In: Veterinary Dermatology, Vol. 17, No. 1, 02.2006, p. 17-23.

Research output: Contribution to journalArticle

Bovell, Douglas ; Lindsay, Susan L. ; Corbett, Alistair D. ; Steel, Catherine. / Immunolocalization of aquaporin-5 expression in sweat gland cells from normal and anhidrotic horses. In: Veterinary Dermatology. 2006 ; Vol. 17, No. 1. pp. 17-23.
@article{c022876e2b934af6bad93b31dd9afb8f,
title = "Immunolocalization of aquaporin-5 expression in sweat gland cells from normal and anhidrotic horses",
abstract = "Western blot analysis showed that sweat gland cells from freely sweating horses expressed the water channel aquaporin-5 (AQP-5). Immunohistochemistry revealed a strong AQP-5-like activity reaction at the apical membrane of the glandular secretory cells, which was absent from the surrounding myoepithelium and all other skin structures. In anhidrotic horses, AQP-5 was also found at the apical membrane of the luminal sweat gland cells, but the level of expression reduced with the length of time that the animal had displayed anhidrosis. The level of AQP-5 expression was substantially reduced in animals with long-term anhidrosis, hence implicating water channel impairment as a possible factor in the development of this disorder.",
author = "Douglas Bovell and Lindsay, {Susan L.} and Corbett, {Alistair D.} and Catherine Steel",
year = "2006",
month = "2",
doi = "10.1111/j.1365-3164.2005.00498.x",
language = "English",
volume = "17",
pages = "17--23",
journal = "Veterinary Dermatology",
issn = "0959-4493",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Immunolocalization of aquaporin-5 expression in sweat gland cells from normal and anhidrotic horses

AU - Bovell, Douglas

AU - Lindsay, Susan L.

AU - Corbett, Alistair D.

AU - Steel, Catherine

PY - 2006/2

Y1 - 2006/2

N2 - Western blot analysis showed that sweat gland cells from freely sweating horses expressed the water channel aquaporin-5 (AQP-5). Immunohistochemistry revealed a strong AQP-5-like activity reaction at the apical membrane of the glandular secretory cells, which was absent from the surrounding myoepithelium and all other skin structures. In anhidrotic horses, AQP-5 was also found at the apical membrane of the luminal sweat gland cells, but the level of expression reduced with the length of time that the animal had displayed anhidrosis. The level of AQP-5 expression was substantially reduced in animals with long-term anhidrosis, hence implicating water channel impairment as a possible factor in the development of this disorder.

AB - Western blot analysis showed that sweat gland cells from freely sweating horses expressed the water channel aquaporin-5 (AQP-5). Immunohistochemistry revealed a strong AQP-5-like activity reaction at the apical membrane of the glandular secretory cells, which was absent from the surrounding myoepithelium and all other skin structures. In anhidrotic horses, AQP-5 was also found at the apical membrane of the luminal sweat gland cells, but the level of expression reduced with the length of time that the animal had displayed anhidrosis. The level of AQP-5 expression was substantially reduced in animals with long-term anhidrosis, hence implicating water channel impairment as a possible factor in the development of this disorder.

UR - http://www.scopus.com/inward/record.url?scp=33644916081&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644916081&partnerID=8YFLogxK

U2 - 10.1111/j.1365-3164.2005.00498.x

DO - 10.1111/j.1365-3164.2005.00498.x

M3 - Article

C2 - 16412116

AN - SCOPUS:33644916081

VL - 17

SP - 17

EP - 23

JO - Veterinary Dermatology

JF - Veterinary Dermatology

SN - 0959-4493

IS - 1

ER -