Immunohistochemistry of normal human knee synovium: A quantitative study

J. A. Singh, Thurayya Arayssi, P. Duray, H. R. Schumacher

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Objective: To describe the immunohistochemical characteristics of knee synovium from normal healthy subjects. Methods: 12 healthy subjects underwent needle biopsy of knee synovium. Using antibodies directed against CD3, CD4, CD8, L26, Kp-1, and HLA-DR, detailed quantitative immunohistochemical analysis of various cell subpopulations was undertaken. Results: The mean (SD) age of the subjects was 37 (9) years (five male, seven female). All had a negative history for arthritis, no knee pain, and a totally normal joint examination except for the presence of retropatellar crepitus in five. For technical reasons staining for all immunohistochemical markers could not be achieved in all subjects. CD3+ T lymphocytes were seen in nine of 10 subjects, either diffusely or, more commonly, in perivascular areas. CD4+ cells were seen in synovium in three of seven subjects and CD8+ cells in six of eight subjects, in almost equal numbers (CD4:CD8, 1.1:1). L26+B lymphocytes were not seen in any biopsy. Kp1+ macrophages were found in 10 of 10 subjects, predominantly in surface lining cells, and in small numbers in diffuse and perivascular locations. HLA-DR+ cells were seen in 10 of 10 subjects, predominantly in surface lining cells and diffusely, but a few were seen perivascularly. Conclusions: Synovium from apparently normal subjects contained a wide range of different cell subpopulations but no B cells. The significance of these immune cells in normal synovium is unclear. A better understanding of their role in normal synovium may be important in analysing the transition to synovitis.

Original languageEnglish
Pages (from-to)785-790
Number of pages6
JournalAnnals of the Rheumatic Diseases
Volume63
Issue number7
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

Fingerprint

Synovial Membrane
Biopsy
HLA-DR Antigens
Linings
Knee
Immunohistochemistry
T-cells
Lymphocytes
Macrophages
Needles
Cells
Antibodies
Chemical analysis
Healthy Volunteers
B-Lymphocytes
Synovitis
Needle Biopsy
Arthritis
Joints
History

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Immunohistochemistry of normal human knee synovium : A quantitative study. / Singh, J. A.; Arayssi, Thurayya; Duray, P.; Schumacher, H. R.

In: Annals of the Rheumatic Diseases, Vol. 63, No. 7, 07.2004, p. 785-790.

Research output: Contribution to journalArticle

Singh, J. A. ; Arayssi, Thurayya ; Duray, P. ; Schumacher, H. R. / Immunohistochemistry of normal human knee synovium : A quantitative study. In: Annals of the Rheumatic Diseases. 2004 ; Vol. 63, No. 7. pp. 785-790.
@article{8cf18727ca0547378ffc4e2fbad0f5ae,
title = "Immunohistochemistry of normal human knee synovium: A quantitative study",
abstract = "Objective: To describe the immunohistochemical characteristics of knee synovium from normal healthy subjects. Methods: 12 healthy subjects underwent needle biopsy of knee synovium. Using antibodies directed against CD3, CD4, CD8, L26, Kp-1, and HLA-DR, detailed quantitative immunohistochemical analysis of various cell subpopulations was undertaken. Results: The mean (SD) age of the subjects was 37 (9) years (five male, seven female). All had a negative history for arthritis, no knee pain, and a totally normal joint examination except for the presence of retropatellar crepitus in five. For technical reasons staining for all immunohistochemical markers could not be achieved in all subjects. CD3+ T lymphocytes were seen in nine of 10 subjects, either diffusely or, more commonly, in perivascular areas. CD4+ cells were seen in synovium in three of seven subjects and CD8+ cells in six of eight subjects, in almost equal numbers (CD4:CD8, 1.1:1). L26+B lymphocytes were not seen in any biopsy. Kp1+ macrophages were found in 10 of 10 subjects, predominantly in surface lining cells, and in small numbers in diffuse and perivascular locations. HLA-DR+ cells were seen in 10 of 10 subjects, predominantly in surface lining cells and diffusely, but a few were seen perivascularly. Conclusions: Synovium from apparently normal subjects contained a wide range of different cell subpopulations but no B cells. The significance of these immune cells in normal synovium is unclear. A better understanding of their role in normal synovium may be important in analysing the transition to synovitis.",
author = "Singh, {J. A.} and Thurayya Arayssi and P. Duray and Schumacher, {H. R.}",
year = "2004",
month = "7",
doi = "10.1136/ard.2003.013383",
language = "English",
volume = "63",
pages = "785--790",
journal = "Annals of the Rheumatic Diseases",
issn = "0003-4967",
publisher = "BMJ Publishing Group",
number = "7",

}

TY - JOUR

T1 - Immunohistochemistry of normal human knee synovium

T2 - A quantitative study

AU - Singh, J. A.

AU - Arayssi, Thurayya

AU - Duray, P.

AU - Schumacher, H. R.

PY - 2004/7

Y1 - 2004/7

N2 - Objective: To describe the immunohistochemical characteristics of knee synovium from normal healthy subjects. Methods: 12 healthy subjects underwent needle biopsy of knee synovium. Using antibodies directed against CD3, CD4, CD8, L26, Kp-1, and HLA-DR, detailed quantitative immunohistochemical analysis of various cell subpopulations was undertaken. Results: The mean (SD) age of the subjects was 37 (9) years (five male, seven female). All had a negative history for arthritis, no knee pain, and a totally normal joint examination except for the presence of retropatellar crepitus in five. For technical reasons staining for all immunohistochemical markers could not be achieved in all subjects. CD3+ T lymphocytes were seen in nine of 10 subjects, either diffusely or, more commonly, in perivascular areas. CD4+ cells were seen in synovium in three of seven subjects and CD8+ cells in six of eight subjects, in almost equal numbers (CD4:CD8, 1.1:1). L26+B lymphocytes were not seen in any biopsy. Kp1+ macrophages were found in 10 of 10 subjects, predominantly in surface lining cells, and in small numbers in diffuse and perivascular locations. HLA-DR+ cells were seen in 10 of 10 subjects, predominantly in surface lining cells and diffusely, but a few were seen perivascularly. Conclusions: Synovium from apparently normal subjects contained a wide range of different cell subpopulations but no B cells. The significance of these immune cells in normal synovium is unclear. A better understanding of their role in normal synovium may be important in analysing the transition to synovitis.

AB - Objective: To describe the immunohistochemical characteristics of knee synovium from normal healthy subjects. Methods: 12 healthy subjects underwent needle biopsy of knee synovium. Using antibodies directed against CD3, CD4, CD8, L26, Kp-1, and HLA-DR, detailed quantitative immunohistochemical analysis of various cell subpopulations was undertaken. Results: The mean (SD) age of the subjects was 37 (9) years (five male, seven female). All had a negative history for arthritis, no knee pain, and a totally normal joint examination except for the presence of retropatellar crepitus in five. For technical reasons staining for all immunohistochemical markers could not be achieved in all subjects. CD3+ T lymphocytes were seen in nine of 10 subjects, either diffusely or, more commonly, in perivascular areas. CD4+ cells were seen in synovium in three of seven subjects and CD8+ cells in six of eight subjects, in almost equal numbers (CD4:CD8, 1.1:1). L26+B lymphocytes were not seen in any biopsy. Kp1+ macrophages were found in 10 of 10 subjects, predominantly in surface lining cells, and in small numbers in diffuse and perivascular locations. HLA-DR+ cells were seen in 10 of 10 subjects, predominantly in surface lining cells and diffusely, but a few were seen perivascularly. Conclusions: Synovium from apparently normal subjects contained a wide range of different cell subpopulations but no B cells. The significance of these immune cells in normal synovium is unclear. A better understanding of their role in normal synovium may be important in analysing the transition to synovitis.

UR - http://www.scopus.com/inward/record.url?scp=3042824995&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042824995&partnerID=8YFLogxK

U2 - 10.1136/ard.2003.013383

DO - 10.1136/ard.2003.013383

M3 - Article

C2 - 15194572

AN - SCOPUS:3042824995

VL - 63

SP - 785

EP - 790

JO - Annals of the Rheumatic Diseases

JF - Annals of the Rheumatic Diseases

SN - 0003-4967

IS - 7

ER -