Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue

Ali Tiss, Abdelkrim Khadir, Jehad Abubaker, Mohamed Abu-Farha, Irina Al-Khairi, Preethi Cherian, Jeena John, Sina Kavalakatt, Samia Warsame, Fahad Al-Ghimlas, Naser Elkum, Kazem Behbehani, Said Dermime, Mohammed Dehbi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Obesity is characterized by a chronic low-grade inflammation and altered stress responses in key metabolic tissues. Impairment of heat shock response (HSR) has been already linked to diabetes and insulin resistance as reflected by decrease in heat shock proteins (HSPs) expression. However, the status of HSR in non-diabetic human obese has not yet been elucidated. The aim of the current study was to investigate whether obesity triggers a change in the HSR pattern and the impact of physical exercise on this pattern at protein and mRNA levels. Methods. Two groups of adult non-diabetic human subjects consisting of lean and obese (n = 47 for each group) were enrolled in this study. The expression pattern of HSP-27, DNAJB3/HSP-40, HSP-60, HSC-70, HSP72, HSP-90 and GRP-94 in the adipose tissue was primarily investigated by immunohistochemistry and then complemented by western blot and qRT-PCR in Peripheral blood mononuclear cells (PBMCs). HSPs expression levels were correlated with various physical, clinical and biochemical parameters. We have also explored the effect of a 3-month moderate physical exercise on the HSPs expression pattern in obese subjects. Results: Obese subjects displayed increased expression of HSP-60, HSC-70, HSP-72, HSP-90 and GRP-94 and lower expression of DNAJB3/HSP-40 (P < 0.05). No differential expression was observed for HSP-27 between the two groups. Higher levels of HSP-72 and GRP-94 proteins correlated positively with the indices of obesity (body mass index and percent body fat) and circulating levels of IFN-gamma-inducible protein 10 (IP-10) and RANTES chemokines. This expression pattern was concomitant with increased inflammatory response in the adipose tissue as monitored by increased levels of Interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α), and RANTES (P < 0.05). Physical exercise reduced the expression of various HSPs in obese to normal levels observed in lean subjects with a parallel decrease in the endogenous levels of IL-6, TNF-α, and RANTES. Conclusion: Taken together, these data indicate that obesity triggers differential regulation of various components of the HSR in non-diabetic subjects and a 3-month physical moderate exercise was sufficient to restore the normal expression of HSPs in the adipose tissue with concomitant attenuation in the inflammatory response.

Original languageEnglish
Article number106
JournalLipids in Health and Disease
Volume13
Issue number1
DOIs
Publication statusPublished - 1 Jul 2014

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Heat-Shock Response
Heat-Shock Proteins
HSP72 Heat-Shock Proteins
Adipose Tissue
Chemokine CCL5
Tissue
HSP40 Heat-Shock Proteins
Obesity
Exercise
HSP27 Heat-Shock Proteins
Chaperonin 60
HSP90 Heat-Shock Proteins
Interleukin-6
Tumor Necrosis Factor-alpha
Proteins
Medical problems
Chemokines
Insulin Resistance
Blood Cells
Body Mass Index

Keywords

  • Adipose tissue
  • ER stress
  • Exercise
  • Heat shock protein
  • HSP
  • Obesity

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue. / Tiss, Ali; Khadir, Abdelkrim; Abubaker, Jehad; Abu-Farha, Mohamed; Al-Khairi, Irina; Cherian, Preethi; John, Jeena; Kavalakatt, Sina; Warsame, Samia; Al-Ghimlas, Fahad; Elkum, Naser; Behbehani, Kazem; Dermime, Said; Dehbi, Mohammed.

In: Lipids in Health and Disease, Vol. 13, No. 1, 106, 01.07.2014.

Research output: Contribution to journalArticle

Tiss, A, Khadir, A, Abubaker, J, Abu-Farha, M, Al-Khairi, I, Cherian, P, John, J, Kavalakatt, S, Warsame, S, Al-Ghimlas, F, Elkum, N, Behbehani, K, Dermime, S & Dehbi, M 2014, 'Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue', Lipids in Health and Disease, vol. 13, no. 1, 106. https://doi.org/10.1186/1476-511X-13-106
Tiss, Ali ; Khadir, Abdelkrim ; Abubaker, Jehad ; Abu-Farha, Mohamed ; Al-Khairi, Irina ; Cherian, Preethi ; John, Jeena ; Kavalakatt, Sina ; Warsame, Samia ; Al-Ghimlas, Fahad ; Elkum, Naser ; Behbehani, Kazem ; Dermime, Said ; Dehbi, Mohammed. / Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue. In: Lipids in Health and Disease. 2014 ; Vol. 13, No. 1.
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T1 - Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue

AU - Tiss, Ali

AU - Khadir, Abdelkrim

AU - Abubaker, Jehad

AU - Abu-Farha, Mohamed

AU - Al-Khairi, Irina

AU - Cherian, Preethi

AU - John, Jeena

AU - Kavalakatt, Sina

AU - Warsame, Samia

AU - Al-Ghimlas, Fahad

AU - Elkum, Naser

AU - Behbehani, Kazem

AU - Dermime, Said

AU - Dehbi, Mohammed

PY - 2014/7/1

Y1 - 2014/7/1

N2 - Background: Obesity is characterized by a chronic low-grade inflammation and altered stress responses in key metabolic tissues. Impairment of heat shock response (HSR) has been already linked to diabetes and insulin resistance as reflected by decrease in heat shock proteins (HSPs) expression. However, the status of HSR in non-diabetic human obese has not yet been elucidated. The aim of the current study was to investigate whether obesity triggers a change in the HSR pattern and the impact of physical exercise on this pattern at protein and mRNA levels. Methods. Two groups of adult non-diabetic human subjects consisting of lean and obese (n = 47 for each group) were enrolled in this study. The expression pattern of HSP-27, DNAJB3/HSP-40, HSP-60, HSC-70, HSP72, HSP-90 and GRP-94 in the adipose tissue was primarily investigated by immunohistochemistry and then complemented by western blot and qRT-PCR in Peripheral blood mononuclear cells (PBMCs). HSPs expression levels were correlated with various physical, clinical and biochemical parameters. We have also explored the effect of a 3-month moderate physical exercise on the HSPs expression pattern in obese subjects. Results: Obese subjects displayed increased expression of HSP-60, HSC-70, HSP-72, HSP-90 and GRP-94 and lower expression of DNAJB3/HSP-40 (P < 0.05). No differential expression was observed for HSP-27 between the two groups. Higher levels of HSP-72 and GRP-94 proteins correlated positively with the indices of obesity (body mass index and percent body fat) and circulating levels of IFN-gamma-inducible protein 10 (IP-10) and RANTES chemokines. This expression pattern was concomitant with increased inflammatory response in the adipose tissue as monitored by increased levels of Interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α), and RANTES (P < 0.05). Physical exercise reduced the expression of various HSPs in obese to normal levels observed in lean subjects with a parallel decrease in the endogenous levels of IL-6, TNF-α, and RANTES. Conclusion: Taken together, these data indicate that obesity triggers differential regulation of various components of the HSR in non-diabetic subjects and a 3-month physical moderate exercise was sufficient to restore the normal expression of HSPs in the adipose tissue with concomitant attenuation in the inflammatory response.

AB - Background: Obesity is characterized by a chronic low-grade inflammation and altered stress responses in key metabolic tissues. Impairment of heat shock response (HSR) has been already linked to diabetes and insulin resistance as reflected by decrease in heat shock proteins (HSPs) expression. However, the status of HSR in non-diabetic human obese has not yet been elucidated. The aim of the current study was to investigate whether obesity triggers a change in the HSR pattern and the impact of physical exercise on this pattern at protein and mRNA levels. Methods. Two groups of adult non-diabetic human subjects consisting of lean and obese (n = 47 for each group) were enrolled in this study. The expression pattern of HSP-27, DNAJB3/HSP-40, HSP-60, HSC-70, HSP72, HSP-90 and GRP-94 in the adipose tissue was primarily investigated by immunohistochemistry and then complemented by western blot and qRT-PCR in Peripheral blood mononuclear cells (PBMCs). HSPs expression levels were correlated with various physical, clinical and biochemical parameters. We have also explored the effect of a 3-month moderate physical exercise on the HSPs expression pattern in obese subjects. Results: Obese subjects displayed increased expression of HSP-60, HSC-70, HSP-72, HSP-90 and GRP-94 and lower expression of DNAJB3/HSP-40 (P < 0.05). No differential expression was observed for HSP-27 between the two groups. Higher levels of HSP-72 and GRP-94 proteins correlated positively with the indices of obesity (body mass index and percent body fat) and circulating levels of IFN-gamma-inducible protein 10 (IP-10) and RANTES chemokines. This expression pattern was concomitant with increased inflammatory response in the adipose tissue as monitored by increased levels of Interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α), and RANTES (P < 0.05). Physical exercise reduced the expression of various HSPs in obese to normal levels observed in lean subjects with a parallel decrease in the endogenous levels of IL-6, TNF-α, and RANTES. Conclusion: Taken together, these data indicate that obesity triggers differential regulation of various components of the HSR in non-diabetic subjects and a 3-month physical moderate exercise was sufficient to restore the normal expression of HSPs in the adipose tissue with concomitant attenuation in the inflammatory response.

KW - Adipose tissue

KW - ER stress

KW - Exercise

KW - Heat shock protein

KW - HSP

KW - Obesity

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