Immunization through dermal delivery of protein-encoding DNA

A role for migratory dendritic cells

Anne Bouloc, Patricia Walker, Jean-Charles B. Grivel, Jonathan C. Vogel, Stephen I. Katz

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The early mechanisms by which DNA-dependent immunization occurs remain poorly understood. We determined whether intradermal injection of a cytomegalovirus (CMV) promoter driven plasmid encoding hen egg lysozyme (pCMV:HEL) induced sensitization against the encoded protein, and whether cutaneous dendritic cells (DC) were involved in this sensitization. Both humoral and cellular responses to HEL were observed. DC that migrated from skin explant culture 3 days after injection of pCMV:HEL DNA contained mRNA encoding HEL. They induced a 3.5-7-fold increase in [3H]thymidine incorporation by HEL protein-primed CD4+ T cells compared to that induced by DC from mice injected with control plasmid. DC emigrating from skin explants recovered from pCMV:HEL injected mice also sensitized naive mice after adoptive transfer and induced the generation of CTL. Thus following DNA delivery within the dermis, DC can induce primary and secondary immune responses.

Original languageEnglish
Pages (from-to)446-454
Number of pages9
JournalEuropean Journal of Immunology
Volume29
Issue number2
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Langerhans Cells
Dendritic Cells
Immunization
Skin
DNA
Plasmids
Intradermal Injections
Proteins
Adoptive Transfer
Dermis
Cytomegalovirus
Thymidine
T-Lymphocytes
Messenger RNA
Injections

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Immunization through dermal delivery of protein-encoding DNA : A role for migratory dendritic cells. / Bouloc, Anne; Walker, Patricia; Grivel, Jean-Charles B.; Vogel, Jonathan C.; Katz, Stephen I.

In: European Journal of Immunology, Vol. 29, No. 2, 1999, p. 446-454.

Research output: Contribution to journalArticle

Bouloc, Anne ; Walker, Patricia ; Grivel, Jean-Charles B. ; Vogel, Jonathan C. ; Katz, Stephen I. / Immunization through dermal delivery of protein-encoding DNA : A role for migratory dendritic cells. In: European Journal of Immunology. 1999 ; Vol. 29, No. 2. pp. 446-454.
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