Immobilization and enhanced catalytic activity of lipase on modified MWCNT for oily wastewater treatment

Ammar Jamie, Ali S. Alshami, Zuhair O. Maliabari, Muataz Atieh, Othman Charles S. Al Hamouz

Research output: Contribution to journalArticle

7 Citations (Scopus)


In this article, we report results of experiments on covalent immobilization of Candidia rugosa lipase enzyme on modified multiwall carbon nanotubes (MW-CNTs) for oily wastewater treatment application. MWCNTs were produced using chemical vapor deposition (CVD) and surface-modified by nitric acid and organic cross-linkers. Successful attachment and high enzyme loading up to 30 wt % was confirmed via FTIR and TGA analysis. Enzymatic activity and loading, which are dependent on the oxidized MWCNT surfaces, cross-linker types and concentrations, resulted with high thermal and operational stability in the microenvironment conditions. This demonstrates the potential for improved resistance to the severe conditions in industrial applications. Furthermore, the CNTs-immobilized enzyme yielded a catalytic activity about 93 times higher than those immobilized on other reported support materials. Up to 98% biological activity retention was also achieved, marking a significant improvement over literature-reported activities (1–20%). Titrimetric analysis of hydrolyzed samples using MWCNT-Lipase (after 1 hr reaction time at 37°C) resulted in an enzymatic activity increase of about five times over those from lyophilized lipase.

Original languageEnglish
Pages (from-to)1441-1449
Number of pages9
JournalEnvironmental Progress and Sustainable Energy
Issue number5
Publication statusPublished - 1 Sep 2016



  • Candidia rugosa lipase
  • carbon nanotubes
  • immobilization
  • wastewater treatment

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Renewable Energy, Sustainability and the Environment
  • Water Science and Technology
  • Environmental Science(all)
  • Waste Management and Disposal

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