Immobilisation of linear and cyclic RGD-peptides on titanium surfaces and their impact on endothelial cell adhesion and proliferation

P. W. Kämmerer, M. Helle, J. Brieger, M. O. Klein, B. Al-Nawas, M. Gabriel

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Abstract

Functional coatings on titanium vascular stents and endosseous dental implants could probably enhance endothelial cell (EC) adhesion and activity with a shortening of the wound healing time and an increase of peri-implant angiogenesis during early bone formation. Therefore, the role of the structure of linear and cyclic cell adhesive peptides Arg-Gly-Asp (l-RGD and c-RGD) on differently pre-treated titanium (Ti) surfaces (untreated, silanised vs. functionalised with land c-RGD peptides) on EC cell coverage and proliferation was evaluated. After 24 h and after 3 d, surface coverage of adherent cells was quantified and an alamarBlue® proliferation assay was conducted. After 24 h, l-RGD modified surfaces showed a signify cantly better coverage of adhered cells than untreated titanium (p=0.01). Differences between l-RGD surfaces and silanised Ti (p=0.066) as well as between l-RGD and c-RGD surfaces (p=0.191) were not signify cant. After 3 d, c-RGD surfaces showed a signify cant. higher cell coverage than untreated Ti, silanised and l-RGD titanium surfaces (all p<0.0001). After 24 h, c-RGD modified surfaces showed signify cant higher cell proliferation compared to untreated Ti (p=0.003). However, there were no differences in proliferation between c-RGD and l-RGD (p=0.126) or c-RGD and silanised titanium (p=0.196). After 3 d, proliferation on c-RGD surfaces outranged signifycantly untreated titanium (p=0.004), silanised (p=0.001) and l-RGD surfaces (p=0.023), whereas no signify cant difference could be found between untreated Ti and l-RGD surfaces (p=0.54). According to these results, the biomimetic coating of c-RGD peptides on conventional titanium surfaces showed a positive effect on EC cell coverage and proliferation. We were able to show that modifi cations of titanium surfaces with c-RGD are a promising approach in promoting endothelial cell growth.

Original languageEnglish
Pages (from-to)364-372
Number of pages9
JournalEuropean Cells and Materials
Volume21
Publication statusPublished - Jan 2011
Externally publishedYes

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Keywords

  • Cyclic
  • Endothelial cells
  • Immobilisation
  • Linear
  • RGD modification
  • Titanium

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Bioengineering
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Kämmerer, P. W., Helle, M., Brieger, J., Klein, M. O., Al-Nawas, B., & Gabriel, M. (2011). Immobilisation of linear and cyclic RGD-peptides on titanium surfaces and their impact on endothelial cell adhesion and proliferation. European Cells and Materials, 21, 364-372.