IL-10 up-regulates nitric oxide (NO) synthesis by lipopolysaccharide (LPS)-activated macrophages

Improved control of Trypanosoma cruzi infection

F. Jacobs, Damien J. Chaussabel, C. Truyens, V. Leclerq, Y. Carlier, M. Goldman, B. Vray

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We examined the effects of IL-10 on tumour necrosis factor-alpha (TNF- α) and NO production by LPS-activated macrophages and on the ability of these cells to control Trypanosoma cruzi infection. We first observed that the addition of rIL-10 to macrophages of the J774 cell line decreased their synthesis of TNF-α but increased their release of NO in a dose-dependent manner. In parallel, treatment of J774 cells with rIL-10 resulted in a better control of T. cruzi infection involving up-regulation of NO synthesis, as it was not observed in presence of N-nitro-L-arginine methyl ester (L-NAME), a competitive inhibitor of NO synthase. The enhancing effect of rIL-10 on NO production was not observed on peritoneal macrophages from wild-type C57Bl/6 mice, but well on macrophages from IL-10 knock-out mice. The control of NO production by endogenous IL-10 was confirmed by the demonstration that neutralization of IL-10 secreted by LPS-activated macrophages from wild-type mice inhibited their production of NO and, in parallel, their ability to control T. cruzi infection. Taken together, these data demonstrate that both exogenous and endogenous IL-10 up-regulate the production of NO by LPS- activated macrophages and improve thereby their ability to clear T. cruzi infection.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalClinical and Experimental Immunology
Volume113
Issue number1
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Trypanosoma cruzi
Interleukin-10
Lipopolysaccharides
Nitric Oxide
Up-Regulation
Macrophages
Infection
Tumor Necrosis Factor-alpha
NG-Nitroarginine Methyl Ester
Peritoneal Macrophages
Knockout Mice
Nitric Oxide Synthase
Cell Line

Keywords

  • IL-10
  • Lipopolysaccharide
  • Macrophages
  • Nitric oxide
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Immunology

Cite this

IL-10 up-regulates nitric oxide (NO) synthesis by lipopolysaccharide (LPS)-activated macrophages : Improved control of Trypanosoma cruzi infection. / Jacobs, F.; Chaussabel, Damien J.; Truyens, C.; Leclerq, V.; Carlier, Y.; Goldman, M.; Vray, B.

In: Clinical and Experimental Immunology, Vol. 113, No. 1, 1998, p. 59-64.

Research output: Contribution to journalArticle

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