IgG Autoantibodies to "Switch Peptide" Determinants of TCR α/β in Human Pregnancy

Ena Wang, Douglas Lake, John B. Winfield, John J. Marchalonis

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The fetus is a natural allograft that is protected from immunologic rejection by a complex set of structural and regulatory mechanisms. We determined whether healthy pregnant women differed significantly from healthy nonpregnant controls in their capacity to produce autoantibodies to defined antigenic determinants of the α/β T-cell receptor. Although controls and pregnant women expressed comparable levels of autoantibodies against an intact recombinant T-cell receptor containing the complete Vα/Vβ structures, analysis of comparative reactivity against individual peptide segments of the molecules, indicated enhanced reactivity to regions corresponding to the CDR1 of the α chain and to the Fr3 of the variable region of the β chain. A major difference was noted by increased reactivity of IgG autoantibodies of pregnant women to peptides corresponding to the "switch" region joining the variable and constant domains. This was noted with both the Tcr α and β chains and was directed against highly conserved determinants within these molecules. Antibodies to this region are lacking in the non-pregnant controls. It is possible that autoantibodies directed against conserved regions of the T-cell receptor might function in the suppression of T-cell reactivity of fetal determinants.

Original languageEnglish
Pages (from-to)224-228
Number of pages5
JournalClinical Immunology and Immunopathology
Volume73
Issue number2
DOIs
Publication statusPublished - Nov 1994
Externally publishedYes

Fingerprint

Autoantibodies
T-Cell Antigen Receptor
Immunoglobulin G
Pregnant Women
Pregnancy
Peptides
Allografts
Epitopes
Fetus
T-Lymphocytes
Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

Cite this

IgG Autoantibodies to "Switch Peptide" Determinants of TCR α/β in Human Pregnancy. / Wang, Ena; Lake, Douglas; Winfield, John B.; Marchalonis, John J.

In: Clinical Immunology and Immunopathology, Vol. 73, No. 2, 11.1994, p. 224-228.

Research output: Contribution to journalArticle

Wang, Ena ; Lake, Douglas ; Winfield, John B. ; Marchalonis, John J. / IgG Autoantibodies to "Switch Peptide" Determinants of TCR α/β in Human Pregnancy. In: Clinical Immunology and Immunopathology. 1994 ; Vol. 73, No. 2. pp. 224-228.
@article{15c8e424f1ae4775b21b36e1334d9140,
title = "IgG Autoantibodies to {"}Switch Peptide{"} Determinants of TCR α/β in Human Pregnancy",
abstract = "The fetus is a natural allograft that is protected from immunologic rejection by a complex set of structural and regulatory mechanisms. We determined whether healthy pregnant women differed significantly from healthy nonpregnant controls in their capacity to produce autoantibodies to defined antigenic determinants of the α/β T-cell receptor. Although controls and pregnant women expressed comparable levels of autoantibodies against an intact recombinant T-cell receptor containing the complete Vα/Vβ structures, analysis of comparative reactivity against individual peptide segments of the molecules, indicated enhanced reactivity to regions corresponding to the CDR1 of the α chain and to the Fr3 of the variable region of the β chain. A major difference was noted by increased reactivity of IgG autoantibodies of pregnant women to peptides corresponding to the {"}switch{"} region joining the variable and constant domains. This was noted with both the Tcr α and β chains and was directed against highly conserved determinants within these molecules. Antibodies to this region are lacking in the non-pregnant controls. It is possible that autoantibodies directed against conserved regions of the T-cell receptor might function in the suppression of T-cell reactivity of fetal determinants.",
author = "Ena Wang and Douglas Lake and Winfield, {John B.} and Marchalonis, {John J.}",
year = "1994",
month = "11",
doi = "10.1006/clin.1994.1191",
language = "English",
volume = "73",
pages = "224--228",
journal = "Clinical Immunology",
issn = "1521-6616",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - IgG Autoantibodies to "Switch Peptide" Determinants of TCR α/β in Human Pregnancy

AU - Wang, Ena

AU - Lake, Douglas

AU - Winfield, John B.

AU - Marchalonis, John J.

PY - 1994/11

Y1 - 1994/11

N2 - The fetus is a natural allograft that is protected from immunologic rejection by a complex set of structural and regulatory mechanisms. We determined whether healthy pregnant women differed significantly from healthy nonpregnant controls in their capacity to produce autoantibodies to defined antigenic determinants of the α/β T-cell receptor. Although controls and pregnant women expressed comparable levels of autoantibodies against an intact recombinant T-cell receptor containing the complete Vα/Vβ structures, analysis of comparative reactivity against individual peptide segments of the molecules, indicated enhanced reactivity to regions corresponding to the CDR1 of the α chain and to the Fr3 of the variable region of the β chain. A major difference was noted by increased reactivity of IgG autoantibodies of pregnant women to peptides corresponding to the "switch" region joining the variable and constant domains. This was noted with both the Tcr α and β chains and was directed against highly conserved determinants within these molecules. Antibodies to this region are lacking in the non-pregnant controls. It is possible that autoantibodies directed against conserved regions of the T-cell receptor might function in the suppression of T-cell reactivity of fetal determinants.

AB - The fetus is a natural allograft that is protected from immunologic rejection by a complex set of structural and regulatory mechanisms. We determined whether healthy pregnant women differed significantly from healthy nonpregnant controls in their capacity to produce autoantibodies to defined antigenic determinants of the α/β T-cell receptor. Although controls and pregnant women expressed comparable levels of autoantibodies against an intact recombinant T-cell receptor containing the complete Vα/Vβ structures, analysis of comparative reactivity against individual peptide segments of the molecules, indicated enhanced reactivity to regions corresponding to the CDR1 of the α chain and to the Fr3 of the variable region of the β chain. A major difference was noted by increased reactivity of IgG autoantibodies of pregnant women to peptides corresponding to the "switch" region joining the variable and constant domains. This was noted with both the Tcr α and β chains and was directed against highly conserved determinants within these molecules. Antibodies to this region are lacking in the non-pregnant controls. It is possible that autoantibodies directed against conserved regions of the T-cell receptor might function in the suppression of T-cell reactivity of fetal determinants.

UR - http://www.scopus.com/inward/record.url?scp=0028171852&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028171852&partnerID=8YFLogxK

U2 - 10.1006/clin.1994.1191

DO - 10.1006/clin.1994.1191

M3 - Article

VL - 73

SP - 224

EP - 228

JO - Clinical Immunology

JF - Clinical Immunology

SN - 1521-6616

IS - 2

ER -