IFN-γ, but not IP-10, MCP-2 or IL-2 response to RD1 selected peptides associates to active tuberculosis

Delia Goletti, Alamelu Raja, Basirudeen S. Kabeer, Camilla Rodrigues, Archana Sodha, Ornella Butera, Stefania Carrara, Guy Vernet, Christophe Longuet, Giuseppe Ippolito, Satheesh Thangaraj, Marc Leportier, Enrico Girardi, Philippe Henri Lagrange

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Objectives: To evaluate whether in vitro response to Mycobacterium tuberculosis RD1 peptides selected by computational analysis, measured by IFN-γ, IP-10, MCP-2 or IL-2 production, is associated with active tuberculosis (TB) in a country with a high incidence of TB. Methods: 129 individuals were prospectively enrolled, 41 with active-pulmonary TB and 88 without (household contacts and community controls). A whole blood assay based on RD1 selected peptides was performed. Soluble factors were evaluated by ELISA in plasma harvested at day1-post-culture. Enrolled individuals were also tested by QuantiFERON TB-Gold In tube (QFT-IT) and tuberculin skin tests (TST). Results: IFN-γ response to RD1 selected peptides was significantly higher in active TB patients than in household contacts and community controls. IP-10 and MCP-2 response did not differ between active TB patients and household contacts, although it was higher in these groups compared to community controls; conversely IL-2 response did not differ among the three groups. When IFN-γ response to RD1 selected peptides was scored based on receiver-operator-characteristic analysis, active TB was predicted with 68% sensitivity and 86% specificity. QFT-IT and TST showed a sensitivity for active TB of 90% and 68% and a specificity of 58% and 59%, respectively. Conclusions: IFN-γ (but not IP-10, MCP-2 and IL-2) response to RD1 selected peptides is associated with active TB with a higher specificity than QFT-IT and TST.

Original languageEnglish
Pages (from-to)133-143
Number of pages11
JournalJournal of Infection
Volume61
Issue number2
DOIs
Publication statusPublished - Jul 2010
Externally publishedYes

Fingerprint

Interleukin-2
Tuberculosis
Peptides
Tuberculin Test
Skin Tests
Gold
Pulmonary Tuberculosis
Mycobacterium tuberculosis
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity
Incidence

Keywords

  • IGRA
  • IL-2
  • IP-10
  • LTBI
  • MCP-2
  • RD1 peptides
  • Tuberculosis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Medicine(all)

Cite this

IFN-γ, but not IP-10, MCP-2 or IL-2 response to RD1 selected peptides associates to active tuberculosis. / Goletti, Delia; Raja, Alamelu; Kabeer, Basirudeen S.; Rodrigues, Camilla; Sodha, Archana; Butera, Ornella; Carrara, Stefania; Vernet, Guy; Longuet, Christophe; Ippolito, Giuseppe; Thangaraj, Satheesh; Leportier, Marc; Girardi, Enrico; Lagrange, Philippe Henri.

In: Journal of Infection, Vol. 61, No. 2, 07.2010, p. 133-143.

Research output: Contribution to journalArticle

Goletti, D, Raja, A, Kabeer, BS, Rodrigues, C, Sodha, A, Butera, O, Carrara, S, Vernet, G, Longuet, C, Ippolito, G, Thangaraj, S, Leportier, M, Girardi, E & Lagrange, PH 2010, 'IFN-γ, but not IP-10, MCP-2 or IL-2 response to RD1 selected peptides associates to active tuberculosis', Journal of Infection, vol. 61, no. 2, pp. 133-143. https://doi.org/10.1016/j.jinf.2010.05.002
Goletti, Delia ; Raja, Alamelu ; Kabeer, Basirudeen S. ; Rodrigues, Camilla ; Sodha, Archana ; Butera, Ornella ; Carrara, Stefania ; Vernet, Guy ; Longuet, Christophe ; Ippolito, Giuseppe ; Thangaraj, Satheesh ; Leportier, Marc ; Girardi, Enrico ; Lagrange, Philippe Henri. / IFN-γ, but not IP-10, MCP-2 or IL-2 response to RD1 selected peptides associates to active tuberculosis. In: Journal of Infection. 2010 ; Vol. 61, No. 2. pp. 133-143.
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abstract = "Objectives: To evaluate whether in vitro response to Mycobacterium tuberculosis RD1 peptides selected by computational analysis, measured by IFN-γ, IP-10, MCP-2 or IL-2 production, is associated with active tuberculosis (TB) in a country with a high incidence of TB. Methods: 129 individuals were prospectively enrolled, 41 with active-pulmonary TB and 88 without (household contacts and community controls). A whole blood assay based on RD1 selected peptides was performed. Soluble factors were evaluated by ELISA in plasma harvested at day1-post-culture. Enrolled individuals were also tested by QuantiFERON TB-Gold In tube (QFT-IT) and tuberculin skin tests (TST). Results: IFN-γ response to RD1 selected peptides was significantly higher in active TB patients than in household contacts and community controls. IP-10 and MCP-2 response did not differ between active TB patients and household contacts, although it was higher in these groups compared to community controls; conversely IL-2 response did not differ among the three groups. When IFN-γ response to RD1 selected peptides was scored based on receiver-operator-characteristic analysis, active TB was predicted with 68{\%} sensitivity and 86{\%} specificity. QFT-IT and TST showed a sensitivity for active TB of 90{\%} and 68{\%} and a specificity of 58{\%} and 59{\%}, respectively. Conclusions: IFN-γ (but not IP-10, MCP-2 and IL-2) response to RD1 selected peptides is associated with active TB with a higher specificity than QFT-IT and TST.",
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T1 - IFN-γ, but not IP-10, MCP-2 or IL-2 response to RD1 selected peptides associates to active tuberculosis

AU - Goletti, Delia

AU - Raja, Alamelu

AU - Kabeer, Basirudeen S.

AU - Rodrigues, Camilla

AU - Sodha, Archana

AU - Butera, Ornella

AU - Carrara, Stefania

AU - Vernet, Guy

AU - Longuet, Christophe

AU - Ippolito, Giuseppe

AU - Thangaraj, Satheesh

AU - Leportier, Marc

AU - Girardi, Enrico

AU - Lagrange, Philippe Henri

PY - 2010/7

Y1 - 2010/7

N2 - Objectives: To evaluate whether in vitro response to Mycobacterium tuberculosis RD1 peptides selected by computational analysis, measured by IFN-γ, IP-10, MCP-2 or IL-2 production, is associated with active tuberculosis (TB) in a country with a high incidence of TB. Methods: 129 individuals were prospectively enrolled, 41 with active-pulmonary TB and 88 without (household contacts and community controls). A whole blood assay based on RD1 selected peptides was performed. Soluble factors were evaluated by ELISA in plasma harvested at day1-post-culture. Enrolled individuals were also tested by QuantiFERON TB-Gold In tube (QFT-IT) and tuberculin skin tests (TST). Results: IFN-γ response to RD1 selected peptides was significantly higher in active TB patients than in household contacts and community controls. IP-10 and MCP-2 response did not differ between active TB patients and household contacts, although it was higher in these groups compared to community controls; conversely IL-2 response did not differ among the three groups. When IFN-γ response to RD1 selected peptides was scored based on receiver-operator-characteristic analysis, active TB was predicted with 68% sensitivity and 86% specificity. QFT-IT and TST showed a sensitivity for active TB of 90% and 68% and a specificity of 58% and 59%, respectively. Conclusions: IFN-γ (but not IP-10, MCP-2 and IL-2) response to RD1 selected peptides is associated with active TB with a higher specificity than QFT-IT and TST.

AB - Objectives: To evaluate whether in vitro response to Mycobacterium tuberculosis RD1 peptides selected by computational analysis, measured by IFN-γ, IP-10, MCP-2 or IL-2 production, is associated with active tuberculosis (TB) in a country with a high incidence of TB. Methods: 129 individuals were prospectively enrolled, 41 with active-pulmonary TB and 88 without (household contacts and community controls). A whole blood assay based on RD1 selected peptides was performed. Soluble factors were evaluated by ELISA in plasma harvested at day1-post-culture. Enrolled individuals were also tested by QuantiFERON TB-Gold In tube (QFT-IT) and tuberculin skin tests (TST). Results: IFN-γ response to RD1 selected peptides was significantly higher in active TB patients than in household contacts and community controls. IP-10 and MCP-2 response did not differ between active TB patients and household contacts, although it was higher in these groups compared to community controls; conversely IL-2 response did not differ among the three groups. When IFN-γ response to RD1 selected peptides was scored based on receiver-operator-characteristic analysis, active TB was predicted with 68% sensitivity and 86% specificity. QFT-IT and TST showed a sensitivity for active TB of 90% and 68% and a specificity of 58% and 59%, respectively. Conclusions: IFN-γ (but not IP-10, MCP-2 and IL-2) response to RD1 selected peptides is associated with active TB with a higher specificity than QFT-IT and TST.

KW - IGRA

KW - IL-2

KW - IP-10

KW - LTBI

KW - MCP-2

KW - RD1 peptides

KW - Tuberculosis

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