Identification of wild-type and exon 5 deletion variants of estrogen receptor β in normal human mammary gland

Valerie Speirs, Ian P. Adams, David S. Walton, Stephen Atkin

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We have examined messenger RNA (mRNA) expression of estrogen receptor (ER) α wild-type ERβ (mRNA and protein), and ERβ exon 5 deletion variants (ERβ Δ5) in samples of normal human mammary gland obtained from 37 premenopausal subjects undergoing reduction mammoplasty. Comparing individual expressions, ERβ mRNA predominated, expressed in 34 of 37 samples (91%), whereas ERα was found in 21 of 37 cases (57%). Receptor combinations were then analyzed and compared. Most samples either coexpressed ERα with ERβ (54%) or expressed just ERβ (38%). Immunohistochemical analysis revealed that ERβ mRNA expression mirrored that of protein. Immunoreactivity was observed in the nucleus with additional evidence of cytoplastmic staining those epithelial cells lining the breast ducts. Sporadic immunoreactivity was also detected in stromal cells. Expression of wild type and ERβ Δ5 was analyzed, and their expression with ERα was compared. Most samples coexpressed wild-type ERβ and the splice variant (62%; P = 0.05), with 30% exclusively expressing wild-type ERβ. Although samples coexpressing wild type and variant ERβ showed no statistical association with ERα those samples expressing only wild-type ERβ, showed a trend toward associations with ERα (P = 0.07). In conclusion, our data would support a role for ERβ in the normal human mammary gland, where we propose it may be the dominant receptor.

Original languageEnglish
Pages (from-to)1601-1605
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume85
Issue number4
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Human Mammary Glands
Estrogen Receptors
Exons
Messenger RNA
Mammaplasty
Stromal Cells
Linings

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Identification of wild-type and exon 5 deletion variants of estrogen receptor β in normal human mammary gland. / Speirs, Valerie; Adams, Ian P.; Walton, David S.; Atkin, Stephen.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 85, No. 4, 2000, p. 1601-1605.

Research output: Contribution to journalArticle

@article{e1e6c07b838843dabb6c9da0216eb06f,
title = "Identification of wild-type and exon 5 deletion variants of estrogen receptor β in normal human mammary gland",
abstract = "We have examined messenger RNA (mRNA) expression of estrogen receptor (ER) α wild-type ERβ (mRNA and protein), and ERβ exon 5 deletion variants (ERβ Δ5) in samples of normal human mammary gland obtained from 37 premenopausal subjects undergoing reduction mammoplasty. Comparing individual expressions, ERβ mRNA predominated, expressed in 34 of 37 samples (91{\%}), whereas ERα was found in 21 of 37 cases (57{\%}). Receptor combinations were then analyzed and compared. Most samples either coexpressed ERα with ERβ (54{\%}) or expressed just ERβ (38{\%}). Immunohistochemical analysis revealed that ERβ mRNA expression mirrored that of protein. Immunoreactivity was observed in the nucleus with additional evidence of cytoplastmic staining those epithelial cells lining the breast ducts. Sporadic immunoreactivity was also detected in stromal cells. Expression of wild type and ERβ Δ5 was analyzed, and their expression with ERα was compared. Most samples coexpressed wild-type ERβ and the splice variant (62{\%}; P = 0.05), with 30{\%} exclusively expressing wild-type ERβ. Although samples coexpressing wild type and variant ERβ showed no statistical association with ERα those samples expressing only wild-type ERβ, showed a trend toward associations with ERα (P = 0.07). In conclusion, our data would support a role for ERβ in the normal human mammary gland, where we propose it may be the dominant receptor.",
author = "Valerie Speirs and Adams, {Ian P.} and Walton, {David S.} and Stephen Atkin",
year = "2000",
doi = "10.1210/jc.85.4.1601",
language = "English",
volume = "85",
pages = "1601--1605",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "4",

}

TY - JOUR

T1 - Identification of wild-type and exon 5 deletion variants of estrogen receptor β in normal human mammary gland

AU - Speirs, Valerie

AU - Adams, Ian P.

AU - Walton, David S.

AU - Atkin, Stephen

PY - 2000

Y1 - 2000

N2 - We have examined messenger RNA (mRNA) expression of estrogen receptor (ER) α wild-type ERβ (mRNA and protein), and ERβ exon 5 deletion variants (ERβ Δ5) in samples of normal human mammary gland obtained from 37 premenopausal subjects undergoing reduction mammoplasty. Comparing individual expressions, ERβ mRNA predominated, expressed in 34 of 37 samples (91%), whereas ERα was found in 21 of 37 cases (57%). Receptor combinations were then analyzed and compared. Most samples either coexpressed ERα with ERβ (54%) or expressed just ERβ (38%). Immunohistochemical analysis revealed that ERβ mRNA expression mirrored that of protein. Immunoreactivity was observed in the nucleus with additional evidence of cytoplastmic staining those epithelial cells lining the breast ducts. Sporadic immunoreactivity was also detected in stromal cells. Expression of wild type and ERβ Δ5 was analyzed, and their expression with ERα was compared. Most samples coexpressed wild-type ERβ and the splice variant (62%; P = 0.05), with 30% exclusively expressing wild-type ERβ. Although samples coexpressing wild type and variant ERβ showed no statistical association with ERα those samples expressing only wild-type ERβ, showed a trend toward associations with ERα (P = 0.07). In conclusion, our data would support a role for ERβ in the normal human mammary gland, where we propose it may be the dominant receptor.

AB - We have examined messenger RNA (mRNA) expression of estrogen receptor (ER) α wild-type ERβ (mRNA and protein), and ERβ exon 5 deletion variants (ERβ Δ5) in samples of normal human mammary gland obtained from 37 premenopausal subjects undergoing reduction mammoplasty. Comparing individual expressions, ERβ mRNA predominated, expressed in 34 of 37 samples (91%), whereas ERα was found in 21 of 37 cases (57%). Receptor combinations were then analyzed and compared. Most samples either coexpressed ERα with ERβ (54%) or expressed just ERβ (38%). Immunohistochemical analysis revealed that ERβ mRNA expression mirrored that of protein. Immunoreactivity was observed in the nucleus with additional evidence of cytoplastmic staining those epithelial cells lining the breast ducts. Sporadic immunoreactivity was also detected in stromal cells. Expression of wild type and ERβ Δ5 was analyzed, and their expression with ERα was compared. Most samples coexpressed wild-type ERβ and the splice variant (62%; P = 0.05), with 30% exclusively expressing wild-type ERβ. Although samples coexpressing wild type and variant ERβ showed no statistical association with ERα those samples expressing only wild-type ERβ, showed a trend toward associations with ERα (P = 0.07). In conclusion, our data would support a role for ERβ in the normal human mammary gland, where we propose it may be the dominant receptor.

UR - http://www.scopus.com/inward/record.url?scp=0034456099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034456099&partnerID=8YFLogxK

U2 - 10.1210/jc.85.4.1601

DO - 10.1210/jc.85.4.1601

M3 - Article

VL - 85

SP - 1601

EP - 1605

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 4

ER -