Identification of Genetic Variants of Gestational Diabetes in South Indians

Sekar Kanthimathi, Chidambaram Manickam, Samuel Liju, Balaji Bhavadharini, Dhanasekaran Bodhini, Visvanathan G nana Prakash, Anandakumar Amutha, Aruyerchelvan Bhavatharini, Ranjit M ohan Anjana, Viswanathan Mohan, Venkatesan Radha

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Abstract

BACKGROUND: This study examined the association in a South Indian population with gestational diabetes mellitus (GDM) of type 2 diabetes risk variants that have previously conferred susceptibility to GDM in other populations.

SUBJECTS AND METHODS: The study groups comprised 518 women with GDM and 910 pregnant women with normal glucose tolerance (NGT). Women with GDM were recruited from a tertiary diabetes center in Chennai, in south India, and NGT women were selected from antenatal clinics also in Chennai. Genomic DNA was isolated from whole blood using the phenol chloroform method. Twelve previously reported GDM-associated single nucleotide polymorphisms (SNPs) in or near nine loci were genotyped using the MassARRAY™ system (Sequenom, San Diego, CA).

RESULTS: Among the 12 SNPs genotyped, 11 SNPs were in Hardy-Weinberg equilibrium and had a call rate of >95%. Of the 11 SNPs previously associated with GDM in other populations, significant association was observed only with the rs7754840 and rs7756992 SNPs of the CDK5 regulatory subunit associated protein 1-like 1 (CDKAL1) gene in this population. The minor alleles of the SNPs rs7754840 and rs7756992 showed significant susceptibility to GDM with an odds ratio of 1.34 (95% confidence interval, 1.12-1.60; P = 0.0013) and 1.45 (95% confidence interval, 1.21-1.72; P = 0.00004), respectively.

CONCLUSIONS: The rs7754840 and rs7756992 SNPs of the CDKAL1 gene were found to be associated with GDM in this south Indian population. This is the first study describing genetic susceptibility of GDM in Asian Indians.

Original languageEnglish
Pages (from-to)462-467
Number of pages6
JournalDiabetes Technology and Therapeutics
Volume17
Issue number7
DOIs
Publication statusPublished - 1 Jul 2015
Externally publishedYes

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ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Medical Laboratory Technology

Cite this

Kanthimathi, S., Manickam, C., Liju, S., Bhavadharini, B., Bodhini, D., Prakash, V. G. N., Amutha, A., Bhavatharini, A., Anjana, R. M. O., Mohan, V., & Radha, V. (2015). Identification of Genetic Variants of Gestational Diabetes in South Indians. Diabetes Technology and Therapeutics, 17(7), 462-467. https://doi.org/10.1089/dia.2014.0349