Identification of Genetic Variants of Gestational Diabetes in South Indians

Sekar Kanthimathi, Chidambaram Manickam, Samuel Liju, Balaji Bhavadharini, Dhanasekaran Bodhini, Visvanathan G nana Prakash, Anandakumar Amutha, Aruyerchelvan Bhavatharini, Ranjit M ohan Anjana, Viswanathan Mohan, Venkatesan Radha

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

BACKGROUND: This study examined the association in a South Indian population with gestational diabetes mellitus (GDM) of type 2 diabetes risk variants that have previously conferred susceptibility to GDM in other populations.

SUBJECTS AND METHODS: The study groups comprised 518 women with GDM and 910 pregnant women with normal glucose tolerance (NGT). Women with GDM were recruited from a tertiary diabetes center in Chennai, in south India, and NGT women were selected from antenatal clinics also in Chennai. Genomic DNA was isolated from whole blood using the phenol chloroform method. Twelve previously reported GDM-associated single nucleotide polymorphisms (SNPs) in or near nine loci were genotyped using the MassARRAY™ system (Sequenom, San Diego, CA).

RESULTS: Among the 12 SNPs genotyped, 11 SNPs were in Hardy-Weinberg equilibrium and had a call rate of >95%. Of the 11 SNPs previously associated with GDM in other populations, significant association was observed only with the rs7754840 and rs7756992 SNPs of the CDK5 regulatory subunit associated protein 1-like 1 (CDKAL1) gene in this population. The minor alleles of the SNPs rs7754840 and rs7756992 showed significant susceptibility to GDM with an odds ratio of 1.34 (95% confidence interval, 1.12-1.60; P = 0.0013) and 1.45 (95% confidence interval, 1.21-1.72; P = 0.00004), respectively.

CONCLUSIONS: The rs7754840 and rs7756992 SNPs of the CDKAL1 gene were found to be associated with GDM in this south Indian population. This is the first study describing genetic susceptibility of GDM in Asian Indians.

Original languageEnglish
Pages (from-to)462-467
Number of pages6
JournalDiabetes Technology and Therapeutics
Volume17
Issue number7
DOIs
Publication statusPublished - 1 Jul 2015
Externally publishedYes

Fingerprint

Gestational Diabetes
Single Nucleotide Polymorphism
Population
Confidence Intervals
Glucose
Protein Subunits
Genetic Predisposition to Disease
Chloroform
Phenol
Type 2 Diabetes Mellitus
Genes
Pregnant Women
India
Alleles
Odds Ratio
DNA

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Medical Laboratory Technology

Cite this

Kanthimathi, S., Manickam, C., Liju, S., Bhavadharini, B., Bodhini, D., Prakash, V. G. N., ... Radha, V. (2015). Identification of Genetic Variants of Gestational Diabetes in South Indians. Diabetes Technology and Therapeutics, 17(7), 462-467. https://doi.org/10.1089/dia.2014.0349

Identification of Genetic Variants of Gestational Diabetes in South Indians. / Kanthimathi, Sekar; Manickam, Chidambaram; Liju, Samuel; Bhavadharini, Balaji; Bodhini, Dhanasekaran; Prakash, Visvanathan G nana; Amutha, Anandakumar; Bhavatharini, Aruyerchelvan; Anjana, Ranjit M ohan; Mohan, Viswanathan; Radha, Venkatesan.

In: Diabetes Technology and Therapeutics, Vol. 17, No. 7, 01.07.2015, p. 462-467.

Research output: Contribution to journalArticle

Kanthimathi, S, Manickam, C, Liju, S, Bhavadharini, B, Bodhini, D, Prakash, VGN, Amutha, A, Bhavatharini, A, Anjana, RMO, Mohan, V & Radha, V 2015, 'Identification of Genetic Variants of Gestational Diabetes in South Indians', Diabetes Technology and Therapeutics, vol. 17, no. 7, pp. 462-467. https://doi.org/10.1089/dia.2014.0349
Kanthimathi, Sekar ; Manickam, Chidambaram ; Liju, Samuel ; Bhavadharini, Balaji ; Bodhini, Dhanasekaran ; Prakash, Visvanathan G nana ; Amutha, Anandakumar ; Bhavatharini, Aruyerchelvan ; Anjana, Ranjit M ohan ; Mohan, Viswanathan ; Radha, Venkatesan. / Identification of Genetic Variants of Gestational Diabetes in South Indians. In: Diabetes Technology and Therapeutics. 2015 ; Vol. 17, No. 7. pp. 462-467.
@article{445d24d6239c4cb681d62a2ccd3d5d59,
title = "Identification of Genetic Variants of Gestational Diabetes in South Indians",
abstract = "BACKGROUND: This study examined the association in a South Indian population with gestational diabetes mellitus (GDM) of type 2 diabetes risk variants that have previously conferred susceptibility to GDM in other populations.SUBJECTS AND METHODS: The study groups comprised 518 women with GDM and 910 pregnant women with normal glucose tolerance (NGT). Women with GDM were recruited from a tertiary diabetes center in Chennai, in south India, and NGT women were selected from antenatal clinics also in Chennai. Genomic DNA was isolated from whole blood using the phenol chloroform method. Twelve previously reported GDM-associated single nucleotide polymorphisms (SNPs) in or near nine loci were genotyped using the MassARRAY™ system (Sequenom, San Diego, CA).RESULTS: Among the 12 SNPs genotyped, 11 SNPs were in Hardy-Weinberg equilibrium and had a call rate of >95{\%}. Of the 11 SNPs previously associated with GDM in other populations, significant association was observed only with the rs7754840 and rs7756992 SNPs of the CDK5 regulatory subunit associated protein 1-like 1 (CDKAL1) gene in this population. The minor alleles of the SNPs rs7754840 and rs7756992 showed significant susceptibility to GDM with an odds ratio of 1.34 (95{\%} confidence interval, 1.12-1.60; P = 0.0013) and 1.45 (95{\%} confidence interval, 1.21-1.72; P = 0.00004), respectively.CONCLUSIONS: The rs7754840 and rs7756992 SNPs of the CDKAL1 gene were found to be associated with GDM in this south Indian population. This is the first study describing genetic susceptibility of GDM in Asian Indians.",
author = "Sekar Kanthimathi and Chidambaram Manickam and Samuel Liju and Balaji Bhavadharini and Dhanasekaran Bodhini and Prakash, {Visvanathan G nana} and Anandakumar Amutha and Aruyerchelvan Bhavatharini and Anjana, {Ranjit M ohan} and Viswanathan Mohan and Venkatesan Radha",
year = "2015",
month = "7",
day = "1",
doi = "10.1089/dia.2014.0349",
language = "English",
volume = "17",
pages = "462--467",
journal = "Diabetes Technology and Therapeutics",
issn = "1520-9156",
publisher = "Mary Ann Liebert Inc.",
number = "7",

}

TY - JOUR

T1 - Identification of Genetic Variants of Gestational Diabetes in South Indians

AU - Kanthimathi, Sekar

AU - Manickam, Chidambaram

AU - Liju, Samuel

AU - Bhavadharini, Balaji

AU - Bodhini, Dhanasekaran

AU - Prakash, Visvanathan G nana

AU - Amutha, Anandakumar

AU - Bhavatharini, Aruyerchelvan

AU - Anjana, Ranjit M ohan

AU - Mohan, Viswanathan

AU - Radha, Venkatesan

PY - 2015/7/1

Y1 - 2015/7/1

N2 - BACKGROUND: This study examined the association in a South Indian population with gestational diabetes mellitus (GDM) of type 2 diabetes risk variants that have previously conferred susceptibility to GDM in other populations.SUBJECTS AND METHODS: The study groups comprised 518 women with GDM and 910 pregnant women with normal glucose tolerance (NGT). Women with GDM were recruited from a tertiary diabetes center in Chennai, in south India, and NGT women were selected from antenatal clinics also in Chennai. Genomic DNA was isolated from whole blood using the phenol chloroform method. Twelve previously reported GDM-associated single nucleotide polymorphisms (SNPs) in or near nine loci were genotyped using the MassARRAY™ system (Sequenom, San Diego, CA).RESULTS: Among the 12 SNPs genotyped, 11 SNPs were in Hardy-Weinberg equilibrium and had a call rate of >95%. Of the 11 SNPs previously associated with GDM in other populations, significant association was observed only with the rs7754840 and rs7756992 SNPs of the CDK5 regulatory subunit associated protein 1-like 1 (CDKAL1) gene in this population. The minor alleles of the SNPs rs7754840 and rs7756992 showed significant susceptibility to GDM with an odds ratio of 1.34 (95% confidence interval, 1.12-1.60; P = 0.0013) and 1.45 (95% confidence interval, 1.21-1.72; P = 0.00004), respectively.CONCLUSIONS: The rs7754840 and rs7756992 SNPs of the CDKAL1 gene were found to be associated with GDM in this south Indian population. This is the first study describing genetic susceptibility of GDM in Asian Indians.

AB - BACKGROUND: This study examined the association in a South Indian population with gestational diabetes mellitus (GDM) of type 2 diabetes risk variants that have previously conferred susceptibility to GDM in other populations.SUBJECTS AND METHODS: The study groups comprised 518 women with GDM and 910 pregnant women with normal glucose tolerance (NGT). Women with GDM were recruited from a tertiary diabetes center in Chennai, in south India, and NGT women were selected from antenatal clinics also in Chennai. Genomic DNA was isolated from whole blood using the phenol chloroform method. Twelve previously reported GDM-associated single nucleotide polymorphisms (SNPs) in or near nine loci were genotyped using the MassARRAY™ system (Sequenom, San Diego, CA).RESULTS: Among the 12 SNPs genotyped, 11 SNPs were in Hardy-Weinberg equilibrium and had a call rate of >95%. Of the 11 SNPs previously associated with GDM in other populations, significant association was observed only with the rs7754840 and rs7756992 SNPs of the CDK5 regulatory subunit associated protein 1-like 1 (CDKAL1) gene in this population. The minor alleles of the SNPs rs7754840 and rs7756992 showed significant susceptibility to GDM with an odds ratio of 1.34 (95% confidence interval, 1.12-1.60; P = 0.0013) and 1.45 (95% confidence interval, 1.21-1.72; P = 0.00004), respectively.CONCLUSIONS: The rs7754840 and rs7756992 SNPs of the CDKAL1 gene were found to be associated with GDM in this south Indian population. This is the first study describing genetic susceptibility of GDM in Asian Indians.

UR - http://www.scopus.com/inward/record.url?scp=84991217801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991217801&partnerID=8YFLogxK

U2 - 10.1089/dia.2014.0349

DO - 10.1089/dia.2014.0349

M3 - Article

VL - 17

SP - 462

EP - 467

JO - Diabetes Technology and Therapeutics

JF - Diabetes Technology and Therapeutics

SN - 1520-9156

IS - 7

ER -