Identification of differentially expressed proteins in curcumin-treated prostate cancer cell lines

Marie Hélène Teiten, Anthoula Gaigneaux, Sébastien Chateauvieux, Anja Billing, Sébastien Planchon, Fred Fack, Jenny Renaut, Fabienne Mack, Claude P. Muller, Mario Dicato, Marc Diederich

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Due to high prevalence and slow progression of prostate cancer, primary prevention appears to be attractive strategy for its eradication. During the last decade, curcumin (diferuloylmethane), a natural compound from the root of turmeric (Curcuma longa), was described as a potent chemopreventive agent. Curcumin exhibits anti-inflammatory, anticarcinogenic, antiproliferative, antiangiogenic, and antioxidant properties in various cancer cell models. This study was designed to identify proteins involved in the anticancer activity of curcumin in androgen-dependent (22Rv1) and -independent (PC-3) human prostate cancer cell lines using two-dimensional difference in gel electrophoresis (2D-DIGE). Out of 425 differentially expressed spots, we describe here the MALDI-TOF-MS analysis of 192 spots of interest, selected by their expression profile. This approach allowed the identification of 60 differentially expressed proteins (32 in 22Rv1 cells and 47 in PC-3 cells). Nineteen proteins are regulated in both cell lines. Further bioinformatic analysis shows that proteins modulated by curcumin are implicated in protein folding (such as heat-shock protein PPP2R1A; RNA splicing proteins RBM17, DDX39; cell death proteins HMGB1 and NPM1; proteins involved in androgen receptor signaling, NPM1 and FKBP4/FKBP52), and that this compound could have an impact on miR-141, miR-152, and miR-183 expression. Taken together, these data support the hypothesis that curcumin is an interesting chemopreventive agent as it modulates the expression of proteins that potentially contribute to prostate carcinogenesis.

Original languageEnglish
Pages (from-to)289-300
Number of pages12
JournalOMICS A Journal of Integrative Biology
Volume16
Issue number6
DOIs
Publication statusPublished - 1 Jun 2012
Externally publishedYes

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Curcumin
Prostatic Neoplasms
Cells
Cell Line
Two-Dimensional Difference Gel Electrophoresis
Proteins
Curcuma
HMGB1 Protein
RNA Splicing
Protein folding
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Protein Folding
Androgen Receptors
Cell death
Primary Prevention
Bioinformatics
Heat-Shock Proteins
Computational Biology
Electrophoresis
Androgens

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Identification of differentially expressed proteins in curcumin-treated prostate cancer cell lines. / Teiten, Marie Hélène; Gaigneaux, Anthoula; Chateauvieux, Sébastien; Billing, Anja; Planchon, Sébastien; Fack, Fred; Renaut, Jenny; Mack, Fabienne; Muller, Claude P.; Dicato, Mario; Diederich, Marc.

In: OMICS A Journal of Integrative Biology, Vol. 16, No. 6, 01.06.2012, p. 289-300.

Research output: Contribution to journalArticle

Teiten, MH, Gaigneaux, A, Chateauvieux, S, Billing, A, Planchon, S, Fack, F, Renaut, J, Mack, F, Muller, CP, Dicato, M & Diederich, M 2012, 'Identification of differentially expressed proteins in curcumin-treated prostate cancer cell lines', OMICS A Journal of Integrative Biology, vol. 16, no. 6, pp. 289-300. https://doi.org/10.1089/omi.2011.0136
Teiten, Marie Hélène ; Gaigneaux, Anthoula ; Chateauvieux, Sébastien ; Billing, Anja ; Planchon, Sébastien ; Fack, Fred ; Renaut, Jenny ; Mack, Fabienne ; Muller, Claude P. ; Dicato, Mario ; Diederich, Marc. / Identification of differentially expressed proteins in curcumin-treated prostate cancer cell lines. In: OMICS A Journal of Integrative Biology. 2012 ; Vol. 16, No. 6. pp. 289-300.
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