Identification of Bacillus anthracis by a simple protective antigen-specific mAb dot-ELISA

Seetharama S. Konduru, U. Tuteja, P. K. Santhosh, M. K. Lalitha, H. V. Batra

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A simple protective antigen (PA)-reactive mAb dot-ELISA was standardized for confirmation of toxin-producing strains of Bacillus anthracis. Twenty-seven clinical isolates were collected from patients clinically suspected of having anthrax. PA was elaborated from these isolates using Casamino acids medium and the culture medium was boiled to kill the cells. PA in boiled culture supernatants was detected using a dot-ELISA. Of the 27 clinical isolates tested, PA was detected in 24 isolates. This was further confirmed by amplifying the PA gene by PCR. This testing procedure is simple to perform, specific and safer than existing procedures, which are added advantages over existing methods of identification of B. anthracis. This test system could be a valuable tool in confirming clinical and environmental isolates of B. anthracis.

Original languageEnglish
Pages (from-to)47-49
Number of pages3
JournalJournal of Medical Microbiology
Volume52
Issue number1
DOIs
Publication statusPublished - 1 Jan 2003
Externally publishedYes

Fingerprint

Bacillus anthracis
Enzyme-Linked Immunosorbent Assay
Antigens
Anthrax
Culture Media
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Identification of Bacillus anthracis by a simple protective antigen-specific mAb dot-ELISA. / Konduru, Seetharama S.; Tuteja, U.; Santhosh, P. K.; Lalitha, M. K.; Batra, H. V.

In: Journal of Medical Microbiology, Vol. 52, No. 1, 01.01.2003, p. 47-49.

Research output: Contribution to journalArticle

Konduru, Seetharama S. ; Tuteja, U. ; Santhosh, P. K. ; Lalitha, M. K. ; Batra, H. V. / Identification of Bacillus anthracis by a simple protective antigen-specific mAb dot-ELISA. In: Journal of Medical Microbiology. 2003 ; Vol. 52, No. 1. pp. 47-49.
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