Identification of a congenic mouse line with obesity and body length phenotypes

Craig H. Warden, Steven Stone, Sally Chiu, Adam L. Diament, Pablo Corva, Donna Shattuck, Robyn Riley, Steven Hunt, Juliet Easlick, Janis S. Fisler, Juan F. Medrano

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Our primary objective was to discover simplified mouse models corresponding to human obesity linkages. We used the B10.UW- H3b we Pax1 un at/Sn (B10.UW) congenic strain, a subcongenic strain with a reduced UW strain donor region, and their C57BL/ 10SnJ background strain. The congenic and subcongenic UW strain donor regions are on mouse Chr 2. We measured body length [anal-nasal (AN) length], summed fat depot weights normalized for body weight (Adiposity Index, AI), and percentage of body weight that is lipid. The B10.UW congenic and subcongenic strains have significantly smaller AN lengths (p < 0.0001) and have a significantly lower AI and percentage of body weight as fat than the background strain (p < 0.0001). In an F2 intercross of the congenic and background strains, AN and AI were both linked to the distal half of the donor region with LOD scores greater than 19 and 5, respectively. F2 haplotypes identified a minimal region for AN linkage of 0.8 megabases (Mb) that is estimated to express four genes in the current Celera mouse genome assembly. We narrowed the most likely location of the obesity gene to 15 Mb whose homologous genes are all located on human Chr 20 in the region surrounding the centromere. Since a previous study identified human obesity linkage peaking near the centromere, then the B10.UW mice may exhibit obesity due to the homologous gene.

Original languageEnglish
Pages (from-to)460-471
Number of pages12
JournalMammalian Genome
Volume15
Issue number6
DOIs
Publication statusPublished - Jun 2004
Externally publishedYes

Fingerprint

Congenic Mice
Nose
Adiposity
Obesity
Phenotype
Centromere
Body Weight
Genes
Fats
Haplotypes
Genome
Lipids
Weights and Measures

ASJC Scopus subject areas

  • Genetics

Cite this

Warden, C. H., Stone, S., Chiu, S., Diament, A. L., Corva, P., Shattuck, D., ... Medrano, J. F. (2004). Identification of a congenic mouse line with obesity and body length phenotypes. Mammalian Genome, 15(6), 460-471. https://doi.org/10.1007/s00335-004-2353-9

Identification of a congenic mouse line with obesity and body length phenotypes. / Warden, Craig H.; Stone, Steven; Chiu, Sally; Diament, Adam L.; Corva, Pablo; Shattuck, Donna; Riley, Robyn; Hunt, Steven; Easlick, Juliet; Fisler, Janis S.; Medrano, Juan F.

In: Mammalian Genome, Vol. 15, No. 6, 06.2004, p. 460-471.

Research output: Contribution to journalArticle

Warden, CH, Stone, S, Chiu, S, Diament, AL, Corva, P, Shattuck, D, Riley, R, Hunt, S, Easlick, J, Fisler, JS & Medrano, JF 2004, 'Identification of a congenic mouse line with obesity and body length phenotypes', Mammalian Genome, vol. 15, no. 6, pp. 460-471. https://doi.org/10.1007/s00335-004-2353-9
Warden CH, Stone S, Chiu S, Diament AL, Corva P, Shattuck D et al. Identification of a congenic mouse line with obesity and body length phenotypes. Mammalian Genome. 2004 Jun;15(6):460-471. https://doi.org/10.1007/s00335-004-2353-9
Warden, Craig H. ; Stone, Steven ; Chiu, Sally ; Diament, Adam L. ; Corva, Pablo ; Shattuck, Donna ; Riley, Robyn ; Hunt, Steven ; Easlick, Juliet ; Fisler, Janis S. ; Medrano, Juan F. / Identification of a congenic mouse line with obesity and body length phenotypes. In: Mammalian Genome. 2004 ; Vol. 15, No. 6. pp. 460-471.
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