Identification of a chemical probe for NAADP by virtual screening

Edmund Naylor, Abdelilah Arredouani, Sridhar R. Vasudevan, Alexander M. Lewis, Raman Parkesh, Akiko Mizote, Daniel Rosen, Justyn M. Thomas, Minoru Izumi, A. Ganesan, Antony Galione, Grant C. Churchill

Research output: Contribution to journalArticle

187 Citations (Scopus)

Abstract

Research into the biological role of the Ca2+-releasing second messenger NAADP (nicotinic acid adenine dinucleotide phosphate) has been hampered by a lack of chemical probes. To find new chemical probes for exploring NAADP signaling, we turned to virtual screening, which can evaluate millions of molecules rapidly and inexpensively. We used NAADP as the query ligand to screen the chemical library ZINC for compounds with similar three-dimensional shape and electrostatic properties. We tested the top-ranking hits in a sea urchin egg bioassay and found that one hit, Ned-19, blocks NAADP signaling at nanomolar concentrations. In intact cells, Ned-19 blocked NAADP signaling and fluorescently labeled NAADP receptors. Moreover, we show the utility of Ned-19 as a chemical probe by using it to demonstrate that NAADP is a key causal link between glucose sensing and Ca2+ increases in mouse pancreatic beta cells.

Original languageEnglish
Pages (from-to)220-226
Number of pages7
JournalNature Chemical Biology
Volume5
Issue number4
DOIs
Publication statusPublished - 2 Apr 2009
Externally publishedYes

Fingerprint

Small Molecule Libraries
Sea Urchins
Insulin-Secreting Cells
Second Messenger Systems
NAADP
Static Electricity
Biological Assay
Ovum
Ligands
Glucose
Research

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Naylor, E., Arredouani, A., Vasudevan, S. R., Lewis, A. M., Parkesh, R., Mizote, A., ... Churchill, G. C. (2009). Identification of a chemical probe for NAADP by virtual screening. Nature Chemical Biology, 5(4), 220-226. https://doi.org/10.1038/nchembio.150

Identification of a chemical probe for NAADP by virtual screening. / Naylor, Edmund; Arredouani, Abdelilah; Vasudevan, Sridhar R.; Lewis, Alexander M.; Parkesh, Raman; Mizote, Akiko; Rosen, Daniel; Thomas, Justyn M.; Izumi, Minoru; Ganesan, A.; Galione, Antony; Churchill, Grant C.

In: Nature Chemical Biology, Vol. 5, No. 4, 02.04.2009, p. 220-226.

Research output: Contribution to journalArticle

Naylor, E, Arredouani, A, Vasudevan, SR, Lewis, AM, Parkesh, R, Mizote, A, Rosen, D, Thomas, JM, Izumi, M, Ganesan, A, Galione, A & Churchill, GC 2009, 'Identification of a chemical probe for NAADP by virtual screening', Nature Chemical Biology, vol. 5, no. 4, pp. 220-226. https://doi.org/10.1038/nchembio.150
Naylor, Edmund ; Arredouani, Abdelilah ; Vasudevan, Sridhar R. ; Lewis, Alexander M. ; Parkesh, Raman ; Mizote, Akiko ; Rosen, Daniel ; Thomas, Justyn M. ; Izumi, Minoru ; Ganesan, A. ; Galione, Antony ; Churchill, Grant C. / Identification of a chemical probe for NAADP by virtual screening. In: Nature Chemical Biology. 2009 ; Vol. 5, No. 4. pp. 220-226.
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