Identification of a B cell signature associated with renal transplant tolerance in humans

Kenneth A. Newell, Adam Asare, Allan D. Kirk, Trang D. Gisler, Kasia Bourcier, Manikkam Suthanthiran, William J. Burlingham, William H. Marks, Ignacio Sanz, Robert I. Lechler, Maria P. Hernandez-Fuentes, Laurence A. Turka, Vicki L. Seyfert-Margolis

Research output: Contribution to journalArticle

439 Citations (Scopus)

Abstract

Establishing long-term allograft acceptance without the requirement for continuous immunosuppression, a condition known as allograft tolerance, is a highly desirable therapeutic goal in solid organ transplantation. Determining which recipients would benefit from withdrawal or minimization of immunosuppression would be greatly facilitated by biomarkers predictive of tolerance. In this study, we identified the largest reported cohort to our knowledge of tolerant renal transplant recipients, as defined by stable graft function and receiving no immunosuppression for more than 1 year, and compared their gene expression profiles and peripheral blood lymphocyte subsets with those of subjects with stable graft function who are receiving immunosuppressive drugs as well as healthy controls. In addition to being associated with clinical and phenotypic parameters, renal allograft tolerance was strongly associated with a B cell signature using several assays. Tolerant subjects showed increased expression of multiple B cell differentiation genes, and a set of just 3 of these genes distinguished tolerant from nontolerant recipients in a unique test set of samples. This B cell signature was associated with upregulation of CD20 mRNA in urine sediment cells and elevated numbers of peripheral blood naive and transitional B cells in tolerant participants compared with those receiving immunosuppression. These results point to a critical role for B cells in regulating alloimmunity and provide a candidate set of genes for wider-scale screening of renal transplant recipients.

Original languageEnglish
Pages (from-to)1836-1847
Number of pages12
JournalJournal of Clinical Investigation
Volume120
Issue number6
DOIs
Publication statusPublished - 1 Jun 2010
Externally publishedYes

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Immunosuppression
B-Lymphocytes
Transplantation Tolerance
Transplants
Kidney
Genes
B-Lymphoid Precursor Cells
Lymphocyte Subsets
Organ Transplantation
Immunosuppressive Agents
Transcriptome
Allografts
Cell Differentiation
Up-Regulation
Cell Count
Biomarkers
Urine
Messenger RNA
Pharmaceutical Preparations
Transplant Recipients

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Newell, K. A., Asare, A., Kirk, A. D., Gisler, T. D., Bourcier, K., Suthanthiran, M., ... Seyfert-Margolis, V. L. (2010). Identification of a B cell signature associated with renal transplant tolerance in humans. Journal of Clinical Investigation, 120(6), 1836-1847. https://doi.org/10.1172/JCI39933

Identification of a B cell signature associated with renal transplant tolerance in humans. / Newell, Kenneth A.; Asare, Adam; Kirk, Allan D.; Gisler, Trang D.; Bourcier, Kasia; Suthanthiran, Manikkam; Burlingham, William J.; Marks, William H.; Sanz, Ignacio; Lechler, Robert I.; Hernandez-Fuentes, Maria P.; Turka, Laurence A.; Seyfert-Margolis, Vicki L.

In: Journal of Clinical Investigation, Vol. 120, No. 6, 01.06.2010, p. 1836-1847.

Research output: Contribution to journalArticle

Newell, KA, Asare, A, Kirk, AD, Gisler, TD, Bourcier, K, Suthanthiran, M, Burlingham, WJ, Marks, WH, Sanz, I, Lechler, RI, Hernandez-Fuentes, MP, Turka, LA & Seyfert-Margolis, VL 2010, 'Identification of a B cell signature associated with renal transplant tolerance in humans', Journal of Clinical Investigation, vol. 120, no. 6, pp. 1836-1847. https://doi.org/10.1172/JCI39933
Newell, Kenneth A. ; Asare, Adam ; Kirk, Allan D. ; Gisler, Trang D. ; Bourcier, Kasia ; Suthanthiran, Manikkam ; Burlingham, William J. ; Marks, William H. ; Sanz, Ignacio ; Lechler, Robert I. ; Hernandez-Fuentes, Maria P. ; Turka, Laurence A. ; Seyfert-Margolis, Vicki L. / Identification of a B cell signature associated with renal transplant tolerance in humans. In: Journal of Clinical Investigation. 2010 ; Vol. 120, No. 6. pp. 1836-1847.
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