Identification and characterization of human leukocyte antigen class I ligands in renal cell carcinoma cells

Barbara Seliger, Sven P. Dressler, Chiara Massa, Christian V. Recktenwald, Florian Altenberend, Juergen Bukur, Francesco M. Marincola, Ena Wang, Stefan Stevanovic, Rudolf Lichtenfels

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The presentation of tumor antigen-derived peptides by human leukocyte antigen (HLA) class I surface antigens on tumor cells is a key prerequisite to trigger effective T-cell responses in cancer patients. Multiple complementary strategies like cDNA and serological expression cloning, reverse immunology and different 'ome'-based methods have been employed to identify potential T-cell targets. This report focuses on a ligandomic profiling approach leading to the identification of 49 naturally processed HLA class I peptide ligands presented on the cell surface of renal cell carcinoma (RCC) cells. The source proteins of the defined HLA ligands are classified according to their biological function and subcellular localization. Previously established cDNA microarray data of paired tissue specimen of RCC and renal epithelium assessed the transcriptional regulation for 28 source proteins. In addition, HLA-A2-restricted, peptide-specific T cells directed against a HLA ligand derived from sulfiredoxin-1 (SRXN1) were generated, which were able to recognize and lyse ligand-presenting target cells in a HLA class I-restricted manner. Furthermore, tumor-infiltrating T cells isolated from a RCC patient were also able to kill SRXN1 expressing tumor cells. Thus, this experimental strategy might be suited to define potential candidate biomarkers and novel targets for T-cell-based immunotherapies of this disease.

Original languageEnglish
Pages (from-to)2528-2541
Number of pages14
JournalProteomics
Volume11
Issue number12
DOIs
Publication statusPublished - Jun 2011
Externally publishedYes

Fingerprint

HLA Antigens
Renal Cell Carcinoma
T-cells
Cells
Ligands
T-Lymphocytes
Tumors
Peptides
Neoplasms
Complementary DNA
Immunology
Peptide T
Histocompatibility Antigens Class I
Cloning
Biomarkers
Neoplasm Antigens
Surface Antigens
Microarrays
Allergy and Immunology
Oligonucleotide Array Sequence Analysis

Keywords

  • Biomarkers
  • Cell biology
  • HLA antigens
  • Peptide ligands
  • Renal cell carcinoma
  • T-cell response

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Seliger, B., Dressler, S. P., Massa, C., Recktenwald, C. V., Altenberend, F., Bukur, J., ... Lichtenfels, R. (2011). Identification and characterization of human leukocyte antigen class I ligands in renal cell carcinoma cells. Proteomics, 11(12), 2528-2541. https://doi.org/10.1002/pmic.201000486

Identification and characterization of human leukocyte antigen class I ligands in renal cell carcinoma cells. / Seliger, Barbara; Dressler, Sven P.; Massa, Chiara; Recktenwald, Christian V.; Altenberend, Florian; Bukur, Juergen; Marincola, Francesco M.; Wang, Ena; Stevanovic, Stefan; Lichtenfels, Rudolf.

In: Proteomics, Vol. 11, No. 12, 06.2011, p. 2528-2541.

Research output: Contribution to journalArticle

Seliger, B, Dressler, SP, Massa, C, Recktenwald, CV, Altenberend, F, Bukur, J, Marincola, FM, Wang, E, Stevanovic, S & Lichtenfels, R 2011, 'Identification and characterization of human leukocyte antigen class I ligands in renal cell carcinoma cells', Proteomics, vol. 11, no. 12, pp. 2528-2541. https://doi.org/10.1002/pmic.201000486
Seliger B, Dressler SP, Massa C, Recktenwald CV, Altenberend F, Bukur J et al. Identification and characterization of human leukocyte antigen class I ligands in renal cell carcinoma cells. Proteomics. 2011 Jun;11(12):2528-2541. https://doi.org/10.1002/pmic.201000486
Seliger, Barbara ; Dressler, Sven P. ; Massa, Chiara ; Recktenwald, Christian V. ; Altenberend, Florian ; Bukur, Juergen ; Marincola, Francesco M. ; Wang, Ena ; Stevanovic, Stefan ; Lichtenfels, Rudolf. / Identification and characterization of human leukocyte antigen class I ligands in renal cell carcinoma cells. In: Proteomics. 2011 ; Vol. 11, No. 12. pp. 2528-2541.
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