Hyperglycaemia disrupts conducted vasodilation in the resistance vasculature of db/db mice

Hamish A.L. Lemmey, Xi Ye, Hong Ding, Christopher Triggle, Christopher J. Garland, Kim A. Dora

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Vascular dysfunction in small resistance arteries is observed during chronic elevations in blood glucose. Hyperglycaemia-associated effects on endothelium-dependent vasodilation have been well characterized, but effects on conducted vasodilation in the resistance vasculature are not known. Small mesenteric arteries were isolated from healthy and diabetic db/db mice, which were used as a model of chronic hyperglycaemia. Endothelium-dependent vasodilation via the Gq/11-coupled proteinase activated receptor 2 (PAR2) was stimulated with the selective agonist SLIGRL. The Ca2+-sensitive fluorescent indicator fluo-8 reported changes in endothelial cell (EC) [Ca2+]i, and triple cannulated bifurcating mesenteric arteries were used to study conducted vasodilation. Chronic hyperglycaemia did not affect either EC Ca2+ or local vasodilation to SLIGRL. However, both acute and chronic exposure to high glucose or the mannitol osmotic control attenuated conducted vasodilation to 10μM SLIGRL. This investigation demonstrates for the first time that a hypertonic solution containing glucose or mannitol can interfere with the spread of a hyperpolarizing current along the endothelium in a physiological setting. Our findings reiterate the importance of studying the effects of hyperglycaemia in the vasculature, and provide the basis for further studies regarding the modulation of junctional proteins involved in cell to cell communication by diseases such as diabetes.

Original languageEnglish
JournalVascular Pharmacology
DOIs
Publication statusAccepted/In press - 1 Jan 2018

Fingerprint

seryl-leucyl-isoleucyl-glycyl-arginyl-leucine
Vasodilation
Hyperglycemia
Endothelium
Mesenteric Arteries
Mannitol
Hypertonic Glucose Solution
Endothelial Cells
PAR-2 Receptor
Cell Communication
Blood Vessels
Blood Glucose
Arteries
Glucose
Proteins

Keywords

  • Conducted vasodilation
  • Db/db mice
  • EDH
  • Hyperglycaemia
  • Resistance arteries

ASJC Scopus subject areas

  • Physiology
  • Molecular Medicine
  • Pharmacology

Cite this

Hyperglycaemia disrupts conducted vasodilation in the resistance vasculature of db/db mice. / Lemmey, Hamish A.L.; Ye, Xi; Ding, Hong; Triggle, Christopher; Garland, Christopher J.; Dora, Kim A.

In: Vascular Pharmacology, 01.01.2018.

Research output: Contribution to journalArticle

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