Hyperexpression of Foxp3 and IDO during acute rejection of islet allografts

Hua Yang, Ruchuang Ding, Vijay K. Sharma, Fludd Saint Hilaire, Milagros Lagman, Baogui Li, Dolea A. Thomas, Xunrong Luo, Ping Song, Craig Stauffer, Phyllis August, Manikkam Suthanthiran

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20 Citations (Scopus)

Abstract

BACKGROUND. We investigated the hypothesis that Foxp3+ cells are an integral component of antiallograft immunity but are dominated by pathogenic effectors. METHODS. Wild-type H-2b C57BL/6 (B6) mice or B6 mice with a targeted disruption of c-Rel gene (c-Rel-/-) were used as recipients of islet grafts from allogeneic DBA/2 (H-2d) mice or syngeneic B6 mice. We developed kinetic quantitative polymerase chain reaction assays and measured intragraft expression of mRNA for Foxp3, IDO, cytolytic molecules, proinflammatory cytokines, and chemokines/receptors. RESULTS. Intraislet levels of mRNA for Foxp3, IDO, CD3, CD25, tumor necrosis factor-α, RANTES, IP-10, and CXCR3 were highest in DBA/2 islet allografts from WT B6 recipients compared to DBA/2 islet allografts from c-Rel-/- B6 recipients or syngeneic B6 islet grafts from WT B6 mice. The ratio of granzyme B or IFN-gamma to Foxp3 was higher with the DBA/2 islet allografts from the WT B6 recipients compared to DBA/2 islet allografts from c-Rel-/- B6 recipients or B6 islet grafts from WT B6 recipients. CONCLUSIONS. Foxp3+ cells are an integral component of acute rejection of allografts but may be dominated by pathogenic effectors.

Original languageEnglish
Pages (from-to)1643-1647
Number of pages5
JournalTransplantation
Volume83
Issue number12
DOIs
Publication statusPublished - 1 Jun 2007

Keywords

  • Foxp3
  • IDO
  • Islets
  • Rejection

ASJC Scopus subject areas

  • Transplantation

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    Yang, H., Ding, R., Sharma, V. K., Hilaire, F. S., Lagman, M., Li, B., Thomas, D. A., Luo, X., Song, P., Stauffer, C., August, P., & Suthanthiran, M. (2007). Hyperexpression of Foxp3 and IDO during acute rejection of islet allografts. Transplantation, 83(12), 1643-1647. https://doi.org/10.1097/01.tp.0000263991.74052.46