Hydroxyl radical scavengers inhibit human natural killer cell activity

Manikkam Suthanthiran, Scott D. Solomon, Perry S. Williams, Albert L. Rubin, Abraham Novogrodsky, Kurt H. Stenzel

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

As natural killer (NK) cell activity is an essential constituent of host defence systems1 and reactive oxygen intermediates participate in such defence2-4, the effect of scavengers of oxygen radicals on NK cell activity was investigated. Hydroxyl radical (OH·) scavengers5 (dimethyl sulphoxide (DMSO), thiourea, dimethylurea, tetramethylurea, benzoic acid, ethanol, methanol and ethylene glycol) inhibited NK cell activity. Catalase, a scavenger of H2O2, and Superoxide dismutase (SOD), a scavenger of O- 2, either alone or in combination, did not inhibit NK cell activity. Inhibition of the lipoxygenase pathway of arachidonic acid metabolism, a potential source of cellular OH·, with nordihydroguaiaretic acid6 and 5,8,11,14-eicosatetraynoic acid (ETYA)7 resulted in marked inhibition of NK cell activity. Inhibition of the cyclooxygenase pathway7,8 with acetylsalicylic acid or indomethacin had minimal effects on NK cell activity. Taken together, these findings suggest that OH·, possibly generated via the lipoxygenase pathway of arachidonic acid metabolism, is critical for NK cell cytotoxicity.

Original languageEnglish
Pages (from-to)276-278
Number of pages3
JournalNature
Volume307
Issue number5948
DOIs
Publication statusPublished - 1 Dec 1984
Externally publishedYes

Fingerprint

Natural Killer Cells
Hydroxyl Radical
Arachidonate Lipoxygenases
5,8,11,14-Eicosatetraynoic Acid
Thiourea
Benzoic Acid
Ethylene Glycol
Prostaglandin-Endoperoxide Synthases
Dimethyl Sulfoxide
Indomethacin
Catalase
Aspirin
Superoxide Dismutase
Methanol
Reactive Oxygen Species
Ethanol
Oxygen

ASJC Scopus subject areas

  • General

Cite this

Suthanthiran, M., Solomon, S. D., Williams, P. S., Rubin, A. L., Novogrodsky, A., & Stenzel, K. H. (1984). Hydroxyl radical scavengers inhibit human natural killer cell activity. Nature, 307(5948), 276-278. https://doi.org/10.1038/307276a0

Hydroxyl radical scavengers inhibit human natural killer cell activity. / Suthanthiran, Manikkam; Solomon, Scott D.; Williams, Perry S.; Rubin, Albert L.; Novogrodsky, Abraham; Stenzel, Kurt H.

In: Nature, Vol. 307, No. 5948, 01.12.1984, p. 276-278.

Research output: Contribution to journalArticle

Suthanthiran, M, Solomon, SD, Williams, PS, Rubin, AL, Novogrodsky, A & Stenzel, KH 1984, 'Hydroxyl radical scavengers inhibit human natural killer cell activity', Nature, vol. 307, no. 5948, pp. 276-278. https://doi.org/10.1038/307276a0
Suthanthiran M, Solomon SD, Williams PS, Rubin AL, Novogrodsky A, Stenzel KH. Hydroxyl radical scavengers inhibit human natural killer cell activity. Nature. 1984 Dec 1;307(5948):276-278. https://doi.org/10.1038/307276a0
Suthanthiran, Manikkam ; Solomon, Scott D. ; Williams, Perry S. ; Rubin, Albert L. ; Novogrodsky, Abraham ; Stenzel, Kurt H. / Hydroxyl radical scavengers inhibit human natural killer cell activity. In: Nature. 1984 ; Vol. 307, No. 5948. pp. 276-278.
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