HuR and miR-1192 regulate myogenesis by modulating the translation of HMGB1 mRNA

Virginie Dormoy-Raclet, Anne Cammas, Barbara Celona, Xian Jin Lian, Kate Van Der Giessen, Marija Zivojnovic, Silvia Brunelli, Francesca Riuzzi, Guglielmo Sorci, Brian T. Wilhelm, Sergio Di Marco, Rosario Donato, Marco E. Bianchi, Imed Gallouzi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Upon muscle injury, the high mobility group box 1 (HMGB1) protein is upregulated and secreted to initiate reparative responses. Here we show that HMGB1 controls myogenesis both in vitro and in vivo during development and after adult muscle injury. HMGB1 expression in muscle cells is regulated at the translational level: the miRNA miR-1192 inhibits HMGB1 translation and the RNA-binding protein HuR promotes it. HuR binds to a cis-element, HuR binding sites (HuRBS), located in the 3′UTR of the HMGB1 transcript, and at the same time miR-1192 is recruited to an adjacent seed element. The binding of HuR to the HuRBS prevents the recruitment of Argonaute 2 (Ago2), overriding miR-1192-mediated translation inhibition. Depleting HuR reduces myoblast fusion and silencing miR-1192 re-establishes the fusion potential of HuR-depleted cells. We propose that HuR promotes the commitment of myoblasts to myogenesis by enhancing the translation of HMGB1 and suppressing the translation inhibition mediated by miR-1192.

Original languageEnglish
Article number2388
JournalNature Communications
Volume4
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Muscle Development
Myoblasts
Muscle
boxes
Binding Sites
HMGB1 Protein
Muscles
Messenger RNA
RNA-Binding Proteins
Wounds and Injuries
Fusion reactions
MicroRNAs
Muscle Cells
Seeds
muscles
Seed
fusion
Cells
proteins
muscle cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Dormoy-Raclet, V., Cammas, A., Celona, B., Lian, X. J., Van Der Giessen, K., Zivojnovic, M., ... Gallouzi, I. (2013). HuR and miR-1192 regulate myogenesis by modulating the translation of HMGB1 mRNA. Nature Communications, 4, [2388]. https://doi.org/10.1038/ncomms3388

HuR and miR-1192 regulate myogenesis by modulating the translation of HMGB1 mRNA. / Dormoy-Raclet, Virginie; Cammas, Anne; Celona, Barbara; Lian, Xian Jin; Van Der Giessen, Kate; Zivojnovic, Marija; Brunelli, Silvia; Riuzzi, Francesca; Sorci, Guglielmo; Wilhelm, Brian T.; Marco, Sergio Di; Donato, Rosario; Bianchi, Marco E.; Gallouzi, Imed.

In: Nature Communications, Vol. 4, 2388, 2013.

Research output: Contribution to journalArticle

Dormoy-Raclet, V, Cammas, A, Celona, B, Lian, XJ, Van Der Giessen, K, Zivojnovic, M, Brunelli, S, Riuzzi, F, Sorci, G, Wilhelm, BT, Marco, SD, Donato, R, Bianchi, ME & Gallouzi, I 2013, 'HuR and miR-1192 regulate myogenesis by modulating the translation of HMGB1 mRNA', Nature Communications, vol. 4, 2388. https://doi.org/10.1038/ncomms3388
Dormoy-Raclet V, Cammas A, Celona B, Lian XJ, Van Der Giessen K, Zivojnovic M et al. HuR and miR-1192 regulate myogenesis by modulating the translation of HMGB1 mRNA. Nature Communications. 2013;4. 2388. https://doi.org/10.1038/ncomms3388
Dormoy-Raclet, Virginie ; Cammas, Anne ; Celona, Barbara ; Lian, Xian Jin ; Van Der Giessen, Kate ; Zivojnovic, Marija ; Brunelli, Silvia ; Riuzzi, Francesca ; Sorci, Guglielmo ; Wilhelm, Brian T. ; Marco, Sergio Di ; Donato, Rosario ; Bianchi, Marco E. ; Gallouzi, Imed. / HuR and miR-1192 regulate myogenesis by modulating the translation of HMGB1 mRNA. In: Nature Communications. 2013 ; Vol. 4.
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