Humoral immune responses and protective efficacy of sequential B- and T-cell epitopes of v antigen of Yersinia pestis by intranasal immunization in microparticles

Jayaprakash Babu Uppada, Arif Azam Khan, Ajaz Ahmad Bhat, Ranjana Deshmukh, Donthamsetty Nageswara Rao

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Capsular F1 and secretory V antigen are the putative vaccine candidates for plague, caused by Yersinia pestis. Contemplating this, we studied the immunogenicity and protective efficacy of collinearly synthesized B- and T-cell epitopes (B-T constructs) of V antigen entrapped in poly (dl-lactide-co- glycolide) microparticles immunized intranasally using single dose immunization schedule in outbred, H-2b and H-2d mice. High antibody levels were observed in terms of IgG, IgA and SIgA peak titers in sera and mucosal washes to different B-T constructs. The constructs ai, bi and fi especially showed high peak antibody titers ranging from 51,200 to 204,000, which were maintained till day 120 post immunization. IgG/IgA Specific activity in sera and washes correlated well with the peak antibody titers. Moreover, all the B-T constructs showed mixed IgG1 and IgG2a/2b response, variable immunoreactivity as well as memory response with V antigen. B-T constructs, viz ai, ak, bi, fi, di and ik showed comparatively high isotype levels. These constructs showed high immunoreactivity, and good recall response with V antigen. Finally, in vivo protective study in BALB/c mice demonstrated the protective efficacy of three B-T constructs (ai, bi and fi) against lethal doses of Yersinia pestis till day 20 post challenge, while construct 'id' showed partial protection.

Original languageEnglish
Pages (from-to)247-256
Number of pages10
JournalMedical Microbiology and Immunology
Volume198
Issue number4
DOIs
Publication statusPublished - 1 Nov 2009

    Fingerprint

Keywords

  • B-T construct
  • Intranasal
  • Microparticles
  • Mucosal immunity
  • Plague
  • V antigen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)

Cite this