Human metapneumovirus in Jordan: Prevalence and clinical symptoms in hospitalized pediatric patients and molecular virus characterization

Lina M. Qaisy, Mamdoh M. Meqdam, Asem Alkhateeb, Abdallah Al-Shorman, Hiyam O. AL-Rousan, Mohammed S. Al-Mogbel

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Respiratory viral infections account for significant morbidity and mortality especially in young children worldwide. Human metapneumovirus (hMPV) causes illnesses ranging from mild respiratory problems to bronchiolitis and severe pneumonia. From January to December 2007, 220 nasopharyngeal aspirates were collected from children younger ≤13 years old hospitalized with lower respiratory tract infection to detect hMPV by revese transcription-polymerase chain reaction and to clone and sequence the hMPV-positive samples. Human metapneumovirus was detected in 28 (12.7%) specimens with a median age of 7 months (range 1.3 to 24 months). Human metapneumovirus type A and type B were detected in 26 (93%) and 8 (28.6%) of specimens, respectively. Coinfection with hMPV type A and type B was detected in 6 (21.4%) specimens positive for hMPV. The major clinical diagnosis of hMPV-positive patients was bronchiolitis (75%). Human metapneumovirus and hMPV type B were found to be significantly associated with bronchiolitis (P = 0.03 and 0.01, respectively). Human metapneumovirus and hMPV type A were found to be significantly associated with pneumonia (P = 0.004 and 0.002, respectively). The main symptoms in patients infected with hMPV were cough (92.9%), fever (82.1%), and wheezing (78.6%), with a significant association of hMPV type A with fever (P = 0.018). Human metapneumovirus was seasonally distributed; most infections with hMPV were reported in the late winter and early spring. The peak of hMPV incidence was in February (10/28; 35.7%).Sequencing of purified plasmid DNA was performed in forward and reverse direction to confirm the results of hMPV-positive samples which scored 97% identity to hMPV type A genome isolate NL/17/00 and showing C-T variation that had no effect on the amino acid sequence F(Phe)-F(Phe).

Original languageEnglish
Pages (from-to)288-291
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume74
Issue number3
DOIs
Publication statusPublished - Nov 2012
Externally publishedYes

Fingerprint

Metapneumovirus
Jordan
Pediatrics
Viruses
Bronchiolitis
Respiratory Tract Infections
Pneumonia
Fever

Keywords

  • HMPV
  • RT-PCR
  • Sequencing

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Human metapneumovirus in Jordan : Prevalence and clinical symptoms in hospitalized pediatric patients and molecular virus characterization. / Qaisy, Lina M.; Meqdam, Mamdoh M.; Alkhateeb, Asem; Al-Shorman, Abdallah; AL-Rousan, Hiyam O.; Al-Mogbel, Mohammed S.

In: Diagnostic Microbiology and Infectious Disease, Vol. 74, No. 3, 11.2012, p. 288-291.

Research output: Contribution to journalArticle

Qaisy, Lina M. ; Meqdam, Mamdoh M. ; Alkhateeb, Asem ; Al-Shorman, Abdallah ; AL-Rousan, Hiyam O. ; Al-Mogbel, Mohammed S. / Human metapneumovirus in Jordan : Prevalence and clinical symptoms in hospitalized pediatric patients and molecular virus characterization. In: Diagnostic Microbiology and Infectious Disease. 2012 ; Vol. 74, No. 3. pp. 288-291.
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