Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization

Carl E. Hilliker, Martine I. Darville, Magdy S. Aly, Mohamed Chikri, Claude Szpirer, Peter Marynen, Guy G. Rousseau, Jean Jacques Cassiman

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Abstract

Two genes encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were localized in human and rat chromosomes. PFKFB1 (previously PFRX), which encodes the liver and muscle isozymes, was assigned to Xq22-q31 in the rat and to Xq27-q28 in the human by in situ hybridization using probes generated by the polymerase chain reaction. PFKFB2, which encodes the heart isozyme of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, was assigned to chromosome 13 in the rat and to chromosome 1 in the human by hybridization of DNA from somatic cell hybrids. By in situ hybridization, this gene was localized to the regions 13q24-25 in the rat and 1q31 in the human.

Original languageEnglish
Pages (from-to)867-873
Number of pages7
JournalGenomics
Volume10
Issue number4
Publication statusPublished - 1 Aug 1991
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

Cite this

Hilliker, C. E., Darville, M. I., Aly, M. S., Chikri, M., Szpirer, C., Marynen, P., Rousseau, G. G., & Cassiman, J. J. (1991). Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization. Genomics, 10(4), 867-873.