Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization

Carl E. Hilliker, Martine I. Darville, Magdy S. Aly, Mohamed Chikri, Claude Szpirer, Peter Marynen, Guy G. Rousseau, Jean Jacques Cassiman

Research output: Contribution to journalArticle

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Abstract

Two genes encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were localized in human and rat chromosomes. PFKFB1 (previously PFRX), which encodes the liver and muscle isozymes, was assigned to Xq22-q31 in the rat and to Xq27-q28 in the human by in situ hybridization using probes generated by the polymerase chain reaction. PFKFB2, which encodes the heart isozyme of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, was assigned to chromosome 13 in the rat and to chromosome 1 in the human by hybridization of DNA from somatic cell hybrids. By in situ hybridization, this gene was localized to the regions 13q24-25 in the rat and 1q31 in the human.

Original languageEnglish
Pages (from-to)867-873
Number of pages7
JournalGenomics
Volume10
Issue number4
Publication statusPublished - 1 Aug 1991
Externally publishedYes

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Phosphofructokinase-2
Hybrid Cells
In Situ Hybridization
Isoenzymes
Genes
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 1
Human Chromosomes
Muscles
Polymerase Chain Reaction
Liver
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization. / Hilliker, Carl E.; Darville, Martine I.; Aly, Magdy S.; Chikri, Mohamed; Szpirer, Claude; Marynen, Peter; Rousseau, Guy G.; Cassiman, Jean Jacques.

In: Genomics, Vol. 10, No. 4, 01.08.1991, p. 867-873.

Research output: Contribution to journalArticle

Hilliker, CE, Darville, MI, Aly, MS, Chikri, M, Szpirer, C, Marynen, P, Rousseau, GG & Cassiman, JJ 1991, 'Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization', Genomics, vol. 10, no. 4, pp. 867-873.
Hilliker, Carl E. ; Darville, Martine I. ; Aly, Magdy S. ; Chikri, Mohamed ; Szpirer, Claude ; Marynen, Peter ; Rousseau, Guy G. ; Cassiman, Jean Jacques. / Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization. In: Genomics. 1991 ; Vol. 10, No. 4. pp. 867-873.
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abstract = "Two genes encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were localized in human and rat chromosomes. PFKFB1 (previously PFRX), which encodes the liver and muscle isozymes, was assigned to Xq22-q31 in the rat and to Xq27-q28 in the human by in situ hybridization using probes generated by the polymerase chain reaction. PFKFB2, which encodes the heart isozyme of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, was assigned to chromosome 13 in the rat and to chromosome 1 in the human by hybridization of DNA from somatic cell hybrids. By in situ hybridization, this gene was localized to the regions 13q24-25 in the rat and 1q31 in the human.",
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