Hsp-72, a candidate prognostic indicator of heatstroke

Mohammed Dehbi, Engin Baturcam, Abdelmoneim Eldali, Maqbool Ahmed, Aaron Kwaasi, Muhammad Azhar Chishti, Abderrezak Bouchama

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Exposure of rats to environmental heat enhances the expression of heat shock protein-72 (Hsp-72) in most of their organs proportionally to heat stress severity. Pre-induction or over-expression of Hsp-72 prevents organ damage and lethality, suggesting that heat shock proteins (Hsps) may have a pathogenic role in this condition. We investigated the expression profile of Hsps in baboons subjected to environmental heat stress until the core temperature attained 42.5°C (moderate heatstroke) or occurrence of hypotension associated with core temperature ≥ 43.5°C (severe heatstroke). Western blot analysis demonstrated a differential induction of Hsp-72 among organs of heat-stressed animals with the highest induction in the liver and the lowest in lung. Hsp-60 and Hsc-70 expression was similar between control and heat-stressed animals. ELISA studies indicated a marked release of Hsp-72 into the circulation of baboons with severe heatstroke with a peak at 24 h post-heatstroke onset and remained sustained up to 72 h. Hsp-72 release was not associated with core temperature or systolic blood pressure, but correlated with markers of liver, myocardium, and skeletal muscle tissue necrosis. Non-survivors displayed significantly higher Hsp-72 levels than survivors. No Hsp-60 was detected in the circulation. These findings add further evidence that increased expression of Hsp-72 may be an important component of the host response to severe heatstroke. They also suggest that extracellular Hsp-72 is a marker of multiple organs tissue damage. Whether extracellular Hsp-72 plays a role in the host immune response to heat stress merits further studies.

Original languageEnglish
Pages (from-to)593-603
Number of pages11
JournalCell Stress and Chaperones
Volume15
Issue number5
DOIs
Publication statusPublished - 1 Sep 2010
Externally publishedYes

Fingerprint

HSP72 Heat-Shock Proteins
Heat Stroke
Hot Temperature
Papio
Heat-Shock Proteins
Liver
Temperature
Animals
Tissue
Blood Pressure
Heat-Shock Response
Blood pressure
Hypotension
Muscle
Rats
Myocardium
Skeletal Muscle
Necrosis
Western Blotting
Enzyme-Linked Immunosorbent Assay

Keywords

  • Baboon
  • Heat shock proteins
  • Heat stress
  • Heatstroke
  • Hyperthermia
  • Multiple organ dysfunction syndrome

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Dehbi, M., Baturcam, E., Eldali, A., Ahmed, M., Kwaasi, A., Chishti, M. A., & Bouchama, A. (2010). Hsp-72, a candidate prognostic indicator of heatstroke. Cell Stress and Chaperones, 15(5), 593-603. https://doi.org/10.1007/s12192-010-0172-3

Hsp-72, a candidate prognostic indicator of heatstroke. / Dehbi, Mohammed; Baturcam, Engin; Eldali, Abdelmoneim; Ahmed, Maqbool; Kwaasi, Aaron; Chishti, Muhammad Azhar; Bouchama, Abderrezak.

In: Cell Stress and Chaperones, Vol. 15, No. 5, 01.09.2010, p. 593-603.

Research output: Contribution to journalArticle

Dehbi, M, Baturcam, E, Eldali, A, Ahmed, M, Kwaasi, A, Chishti, MA & Bouchama, A 2010, 'Hsp-72, a candidate prognostic indicator of heatstroke', Cell Stress and Chaperones, vol. 15, no. 5, pp. 593-603. https://doi.org/10.1007/s12192-010-0172-3
Dehbi M, Baturcam E, Eldali A, Ahmed M, Kwaasi A, Chishti MA et al. Hsp-72, a candidate prognostic indicator of heatstroke. Cell Stress and Chaperones. 2010 Sep 1;15(5):593-603. https://doi.org/10.1007/s12192-010-0172-3
Dehbi, Mohammed ; Baturcam, Engin ; Eldali, Abdelmoneim ; Ahmed, Maqbool ; Kwaasi, Aaron ; Chishti, Muhammad Azhar ; Bouchama, Abderrezak. / Hsp-72, a candidate prognostic indicator of heatstroke. In: Cell Stress and Chaperones. 2010 ; Vol. 15, No. 5. pp. 593-603.
@article{8b741c8dd4bb4a79a26b8848e9a4399f,
title = "Hsp-72, a candidate prognostic indicator of heatstroke",
abstract = "Exposure of rats to environmental heat enhances the expression of heat shock protein-72 (Hsp-72) in most of their organs proportionally to heat stress severity. Pre-induction or over-expression of Hsp-72 prevents organ damage and lethality, suggesting that heat shock proteins (Hsps) may have a pathogenic role in this condition. We investigated the expression profile of Hsps in baboons subjected to environmental heat stress until the core temperature attained 42.5°C (moderate heatstroke) or occurrence of hypotension associated with core temperature ≥ 43.5°C (severe heatstroke). Western blot analysis demonstrated a differential induction of Hsp-72 among organs of heat-stressed animals with the highest induction in the liver and the lowest in lung. Hsp-60 and Hsc-70 expression was similar between control and heat-stressed animals. ELISA studies indicated a marked release of Hsp-72 into the circulation of baboons with severe heatstroke with a peak at 24 h post-heatstroke onset and remained sustained up to 72 h. Hsp-72 release was not associated with core temperature or systolic blood pressure, but correlated with markers of liver, myocardium, and skeletal muscle tissue necrosis. Non-survivors displayed significantly higher Hsp-72 levels than survivors. No Hsp-60 was detected in the circulation. These findings add further evidence that increased expression of Hsp-72 may be an important component of the host response to severe heatstroke. They also suggest that extracellular Hsp-72 is a marker of multiple organs tissue damage. Whether extracellular Hsp-72 plays a role in the host immune response to heat stress merits further studies.",
keywords = "Baboon, Heat shock proteins, Heat stress, Heatstroke, Hyperthermia, Multiple organ dysfunction syndrome",
author = "Mohammed Dehbi and Engin Baturcam and Abdelmoneim Eldali and Maqbool Ahmed and Aaron Kwaasi and Chishti, {Muhammad Azhar} and Abderrezak Bouchama",
year = "2010",
month = "9",
day = "1",
doi = "10.1007/s12192-010-0172-3",
language = "English",
volume = "15",
pages = "593--603",
journal = "Cell Stress and Chaperones",
issn = "1355-8145",
publisher = "Springer Netherlands",
number = "5",

}

TY - JOUR

T1 - Hsp-72, a candidate prognostic indicator of heatstroke

AU - Dehbi, Mohammed

AU - Baturcam, Engin

AU - Eldali, Abdelmoneim

AU - Ahmed, Maqbool

AU - Kwaasi, Aaron

AU - Chishti, Muhammad Azhar

AU - Bouchama, Abderrezak

PY - 2010/9/1

Y1 - 2010/9/1

N2 - Exposure of rats to environmental heat enhances the expression of heat shock protein-72 (Hsp-72) in most of their organs proportionally to heat stress severity. Pre-induction or over-expression of Hsp-72 prevents organ damage and lethality, suggesting that heat shock proteins (Hsps) may have a pathogenic role in this condition. We investigated the expression profile of Hsps in baboons subjected to environmental heat stress until the core temperature attained 42.5°C (moderate heatstroke) or occurrence of hypotension associated with core temperature ≥ 43.5°C (severe heatstroke). Western blot analysis demonstrated a differential induction of Hsp-72 among organs of heat-stressed animals with the highest induction in the liver and the lowest in lung. Hsp-60 and Hsc-70 expression was similar between control and heat-stressed animals. ELISA studies indicated a marked release of Hsp-72 into the circulation of baboons with severe heatstroke with a peak at 24 h post-heatstroke onset and remained sustained up to 72 h. Hsp-72 release was not associated with core temperature or systolic blood pressure, but correlated with markers of liver, myocardium, and skeletal muscle tissue necrosis. Non-survivors displayed significantly higher Hsp-72 levels than survivors. No Hsp-60 was detected in the circulation. These findings add further evidence that increased expression of Hsp-72 may be an important component of the host response to severe heatstroke. They also suggest that extracellular Hsp-72 is a marker of multiple organs tissue damage. Whether extracellular Hsp-72 plays a role in the host immune response to heat stress merits further studies.

AB - Exposure of rats to environmental heat enhances the expression of heat shock protein-72 (Hsp-72) in most of their organs proportionally to heat stress severity. Pre-induction or over-expression of Hsp-72 prevents organ damage and lethality, suggesting that heat shock proteins (Hsps) may have a pathogenic role in this condition. We investigated the expression profile of Hsps in baboons subjected to environmental heat stress until the core temperature attained 42.5°C (moderate heatstroke) or occurrence of hypotension associated with core temperature ≥ 43.5°C (severe heatstroke). Western blot analysis demonstrated a differential induction of Hsp-72 among organs of heat-stressed animals with the highest induction in the liver and the lowest in lung. Hsp-60 and Hsc-70 expression was similar between control and heat-stressed animals. ELISA studies indicated a marked release of Hsp-72 into the circulation of baboons with severe heatstroke with a peak at 24 h post-heatstroke onset and remained sustained up to 72 h. Hsp-72 release was not associated with core temperature or systolic blood pressure, but correlated with markers of liver, myocardium, and skeletal muscle tissue necrosis. Non-survivors displayed significantly higher Hsp-72 levels than survivors. No Hsp-60 was detected in the circulation. These findings add further evidence that increased expression of Hsp-72 may be an important component of the host response to severe heatstroke. They also suggest that extracellular Hsp-72 is a marker of multiple organs tissue damage. Whether extracellular Hsp-72 plays a role in the host immune response to heat stress merits further studies.

KW - Baboon

KW - Heat shock proteins

KW - Heat stress

KW - Heatstroke

KW - Hyperthermia

KW - Multiple organ dysfunction syndrome

UR - http://www.scopus.com/inward/record.url?scp=77955716785&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955716785&partnerID=8YFLogxK

U2 - 10.1007/s12192-010-0172-3

DO - 10.1007/s12192-010-0172-3

M3 - Article

C2 - 20174993

AN - SCOPUS:77955716785

VL - 15

SP - 593

EP - 603

JO - Cell Stress and Chaperones

JF - Cell Stress and Chaperones

SN - 1355-8145

IS - 5

ER -