HPV detection and genotyping using the luminex xMAP technology

Marios Kambouris, Vasiliki Chini, Andriani Daskalaki

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The Human Papillomavirus (HPV) is playing an important role in oral cancer. The molecular detection of HPV is based on the fact that the viral DNA is present in all the epithelial layers of the affected tissue and it can be detected easily with PCR or Real-Time PCR. The major disadvantage of PCR is that it cannot provide genotype information and the Real-Time PCR can only detect very few types in a multiplex assay. Although the HPV typing assays are capable of typing a relatively large spectrum of HPV genotypes, they cannot be automated or deployed in a high-throughput platform. The bead-based technology (Luminex suspension array technology) provides a rapid and cost-effective method to simultaneously detect different HPV genotypes.

Original languageEnglish
Title of host publicationInformatics in Oral Medicine: Advanced Techniques in Clinical and Diagnostic Technologies
PublisherIGI Global
Pages83-87
Number of pages5
ISBN (Print)9781605667331
DOIs
Publication statusPublished - 2010

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ASJC Scopus subject areas

  • Health Professions(all)

Cite this

Kambouris, M., Chini, V., & Daskalaki, A. (2010). HPV detection and genotyping using the luminex xMAP technology. In Informatics in Oral Medicine: Advanced Techniques in Clinical and Diagnostic Technologies (pp. 83-87). IGI Global. https://doi.org/10.4018/978-1-60566-733-1.ch006