HIV-1-induced activation of CD4+ T cells creates new targets for HIV-1 infection in human lymphoid tissue ex vivo

Angélique Biancotto, Sarah J. Iglehart, Christophe Vanpouille, Cristian E. Condack, Andrea Lisco, Elke Ruecker, Ivan Hirsch, Leonid B. Margolis, Jean-Charles B. Grivel

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We demonstrate mechanisms by which HIV-1 appears to facilitate its own infection in ex vivo-infected human lymphoid tissue. In this system, HIV-1 readily infects various CD4+ T cells, but productive viral infection was supported predominantly by activated T cells expressing either CD25 or HLA-DR or both (CD25/HLA-DR) but not other activation markers: There was a strong positive correlation (r = 0.64, P = .001) between virus production and the number of CD25+/HLA-DR+ T cells. HIV-1 infection of lymphoid tissue was associated with activation of both HIV-1-infected and uninfected (bystanders) T cells. In these tissues, apoptosis was selectively increased in T cells expressing CD25/HLA-DR and p24gag but not in cells expressing either of these markers alone. In the course of HIV-1 infection, there was a significant increase in the number of activated (CD25 +/HLA-DR+) T cells both infected and uninfected (bystander). By inducing T cells to express particular markers of activation that create new targets for infection, HIV-1 generates in ex vivo lymphoid tissues a vicious destructive circle of activation and infection. In vivo, such self-perpetuating cycle could contribute to HIV-1 disease.

Original languageEnglish
Pages (from-to)699-704
Number of pages6
JournalBlood
Volume111
Issue number2
DOIs
Publication statusPublished - 15 Jan 2008
Externally publishedYes

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T-cells
Lymphoid Tissue
HIV Infections
HIV-1
Chemical activation
Tissue
T-Lymphocytes
HLA-DR Antigens
Infection
Virus Diseases
Viruses
Cells
Apoptosis

ASJC Scopus subject areas

  • Immunology
  • Biochemistry
  • Hematology
  • Cell Biology

Cite this

HIV-1-induced activation of CD4+ T cells creates new targets for HIV-1 infection in human lymphoid tissue ex vivo. / Biancotto, Angélique; Iglehart, Sarah J.; Vanpouille, Christophe; Condack, Cristian E.; Lisco, Andrea; Ruecker, Elke; Hirsch, Ivan; Margolis, Leonid B.; Grivel, Jean-Charles B.

In: Blood, Vol. 111, No. 2, 15.01.2008, p. 699-704.

Research output: Contribution to journalArticle

Biancotto, A, Iglehart, SJ, Vanpouille, C, Condack, CE, Lisco, A, Ruecker, E, Hirsch, I, Margolis, LB & Grivel, J-CB 2008, 'HIV-1-induced activation of CD4+ T cells creates new targets for HIV-1 infection in human lymphoid tissue ex vivo', Blood, vol. 111, no. 2, pp. 699-704. https://doi.org/10.1182/blood-2007-05-088435
Biancotto A, Iglehart SJ, Vanpouille C, Condack CE, Lisco A, Ruecker E et al. HIV-1-induced activation of CD4+ T cells creates new targets for HIV-1 infection in human lymphoid tissue ex vivo. Blood. 2008 Jan 15;111(2):699-704. https://doi.org/10.1182/blood-2007-05-088435
Biancotto, Angélique ; Iglehart, Sarah J. ; Vanpouille, Christophe ; Condack, Cristian E. ; Lisco, Andrea ; Ruecker, Elke ; Hirsch, Ivan ; Margolis, Leonid B. ; Grivel, Jean-Charles B. / HIV-1-induced activation of CD4+ T cells creates new targets for HIV-1 infection in human lymphoid tissue ex vivo. In: Blood. 2008 ; Vol. 111, No. 2. pp. 699-704.
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