HIV-1-induced activation of CD4+ T cells creates new targets for HIV-1 infection in human lymphoid tissue ex vivo

Angélique Biancotto, Sarah J. Iglehart, Christophe Vanpouille, Cristian E. Condack, Andrea Lisco, Elke Ruecker, Ivan Hirsch, Leonid B. Margolis, Jean Charles Grivel

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Abstract

We demonstrate mechanisms by which HIV-1 appears to facilitate its own infection in ex vivo-infected human lymphoid tissue. In this system, HIV-1 readily infects various CD4+ T cells, but productive viral infection was supported predominantly by activated T cells expressing either CD25 or HLA-DR or both (CD25/HLA-DR) but not other activation markers: There was a strong positive correlation (r = 0.64, P = .001) between virus production and the number of CD25+/HLA-DR+ T cells. HIV-1 infection of lymphoid tissue was associated with activation of both HIV-1-infected and uninfected (bystanders) T cells. In these tissues, apoptosis was selectively increased in T cells expressing CD25/HLA-DR and p24gag but not in cells expressing either of these markers alone. In the course of HIV-1 infection, there was a significant increase in the number of activated (CD25 +/HLA-DR+) T cells both infected and uninfected (bystander). By inducing T cells to express particular markers of activation that create new targets for infection, HIV-1 generates in ex vivo lymphoid tissues a vicious destructive circle of activation and infection. In vivo, such self-perpetuating cycle could contribute to HIV-1 disease.

Original languageEnglish
Pages (from-to)699-704
Number of pages6
JournalBlood
Volume111
Issue number2
DOIs
Publication statusPublished - 15 Jan 2008

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ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Biancotto, A., Iglehart, S. J., Vanpouille, C., Condack, C. E., Lisco, A., Ruecker, E., Hirsch, I., Margolis, L. B., & Grivel, J. C. (2008). HIV-1-induced activation of CD4+ T cells creates new targets for HIV-1 infection in human lymphoid tissue ex vivo. Blood, 111(2), 699-704. https://doi.org/10.1182/blood-2007-05-088435