HIV-1 Expressing the Envelopes of Transmitted/Founder or Control/Reference Viruses Have Similar Infection Patterns of CD4 T-Cells in Human Cervical Tissue Ex Vivo

Melanie Merbah, Anush Arakelyan, Tara Edmonds, Christina Ochsenbauer, John C. Kappes, Robin J. Shattock, Jean-Charles B. Grivel, Leonid B. Margolis

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Recently, it was found that 80% of sexual HIV-1 transmissions are established by a single virion/viral genome. To investigate whether the transmitted/founder (T/F) viruses have specific biological properties favoring sexual transmission, we inoculated human cervical tissue explants with isogenic HIV-1 viruses encoding Env sequences from T/F and control reference (C/R) HIV-1 variants as well as with full length T/F HIV-1 and compared their replication efficiencies, T cell depletion, and the activation status of infected cells. We found that all the HIV-1 variants were capable of transmitting infection to cervical tissue ex vivo and in this system preferentially replicate in activated CD4 T cells and deplete these cells. There was no difference in the biological properties of T/F and C/R HIV-1 variants as evaluated in ex vivo cervical tissue.

Original languageEnglish
Article numbere50839
JournalPLoS One
Volume7
Issue number12
DOIs
Publication statusPublished - 6 Dec 2012
Externally publishedYes

Fingerprint

T-cells
Human immunodeficiency virus 1
Viruses
HIV-1
T-lymphocytes
Tissue
T-Lymphocytes
viruses
Infection
infection
Genes
Chemical activation
Viral Genome
virion
Virion
tissues
explants
cells
genome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

HIV-1 Expressing the Envelopes of Transmitted/Founder or Control/Reference Viruses Have Similar Infection Patterns of CD4 T-Cells in Human Cervical Tissue Ex Vivo. / Merbah, Melanie; Arakelyan, Anush; Edmonds, Tara; Ochsenbauer, Christina; Kappes, John C.; Shattock, Robin J.; Grivel, Jean-Charles B.; Margolis, Leonid B.

In: PLoS One, Vol. 7, No. 12, e50839, 06.12.2012.

Research output: Contribution to journalArticle

Merbah, Melanie ; Arakelyan, Anush ; Edmonds, Tara ; Ochsenbauer, Christina ; Kappes, John C. ; Shattock, Robin J. ; Grivel, Jean-Charles B. ; Margolis, Leonid B. / HIV-1 Expressing the Envelopes of Transmitted/Founder or Control/Reference Viruses Have Similar Infection Patterns of CD4 T-Cells in Human Cervical Tissue Ex Vivo. In: PLoS One. 2012 ; Vol. 7, No. 12.
@article{e02b3fd5af984e33a63bccaa72aa72e7,
title = "HIV-1 Expressing the Envelopes of Transmitted/Founder or Control/Reference Viruses Have Similar Infection Patterns of CD4 T-Cells in Human Cervical Tissue Ex Vivo",
abstract = "Recently, it was found that 80{\%} of sexual HIV-1 transmissions are established by a single virion/viral genome. To investigate whether the transmitted/founder (T/F) viruses have specific biological properties favoring sexual transmission, we inoculated human cervical tissue explants with isogenic HIV-1 viruses encoding Env sequences from T/F and control reference (C/R) HIV-1 variants as well as with full length T/F HIV-1 and compared their replication efficiencies, T cell depletion, and the activation status of infected cells. We found that all the HIV-1 variants were capable of transmitting infection to cervical tissue ex vivo and in this system preferentially replicate in activated CD4 T cells and deplete these cells. There was no difference in the biological properties of T/F and C/R HIV-1 variants as evaluated in ex vivo cervical tissue.",
author = "Melanie Merbah and Anush Arakelyan and Tara Edmonds and Christina Ochsenbauer and Kappes, {John C.} and Shattock, {Robin J.} and Grivel, {Jean-Charles B.} and Margolis, {Leonid B.}",
year = "2012",
month = "12",
day = "6",
doi = "10.1371/journal.pone.0050839",
language = "English",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - HIV-1 Expressing the Envelopes of Transmitted/Founder or Control/Reference Viruses Have Similar Infection Patterns of CD4 T-Cells in Human Cervical Tissue Ex Vivo

AU - Merbah, Melanie

AU - Arakelyan, Anush

AU - Edmonds, Tara

AU - Ochsenbauer, Christina

AU - Kappes, John C.

AU - Shattock, Robin J.

AU - Grivel, Jean-Charles B.

AU - Margolis, Leonid B.

PY - 2012/12/6

Y1 - 2012/12/6

N2 - Recently, it was found that 80% of sexual HIV-1 transmissions are established by a single virion/viral genome. To investigate whether the transmitted/founder (T/F) viruses have specific biological properties favoring sexual transmission, we inoculated human cervical tissue explants with isogenic HIV-1 viruses encoding Env sequences from T/F and control reference (C/R) HIV-1 variants as well as with full length T/F HIV-1 and compared their replication efficiencies, T cell depletion, and the activation status of infected cells. We found that all the HIV-1 variants were capable of transmitting infection to cervical tissue ex vivo and in this system preferentially replicate in activated CD4 T cells and deplete these cells. There was no difference in the biological properties of T/F and C/R HIV-1 variants as evaluated in ex vivo cervical tissue.

AB - Recently, it was found that 80% of sexual HIV-1 transmissions are established by a single virion/viral genome. To investigate whether the transmitted/founder (T/F) viruses have specific biological properties favoring sexual transmission, we inoculated human cervical tissue explants with isogenic HIV-1 viruses encoding Env sequences from T/F and control reference (C/R) HIV-1 variants as well as with full length T/F HIV-1 and compared their replication efficiencies, T cell depletion, and the activation status of infected cells. We found that all the HIV-1 variants were capable of transmitting infection to cervical tissue ex vivo and in this system preferentially replicate in activated CD4 T cells and deplete these cells. There was no difference in the biological properties of T/F and C/R HIV-1 variants as evaluated in ex vivo cervical tissue.

UR - http://www.scopus.com/inward/record.url?scp=84870829395&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870829395&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0050839

DO - 10.1371/journal.pone.0050839

M3 - Article

C2 - 23236398

AN - SCOPUS:84870829395

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 12

M1 - e50839

ER -