Histone acetylation influences both gene expression and development of Xenopus laevis

Geneviève Almouzni, Saadi Khochbin, Stefan Dimitrov, Alan P. Wolffe

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

We examine the potential role of histone hyperacetylation in gene activation during Xenopus development using Trichostatin A, (TSA), a specific inhibitor of histone deacetylase. We find that TSA is very effective in inducing both core histone hyperacetylation and histone H1° gene expression in a Xenopus somatic cell line. In contrast, TSA does not induce histone hyperacetylation or histone H1° transcription in Xenopus oocytes. Histone hyperacetylation is developmentally regulated during Xenopus embryogenesis; hyperacetylated histones first accumulate early in gastrulation. The capacity of TSA to induce histone H1° gene expression correlates with the induction of histone hyperacetylation. Concentrations of TSA sufficient to induce histone hyperacetylation in Xenopus embryos delay gastrulation and cause diminished midtrunk and posterior formation, suggesting defects in mesoderm formation. Although the constitutive hyperacetylation of the histones does not prevent either the cell division or differentiation sufficient for early morphogenesis it has a role in establishing stable states of differential gene activity during gastrulation.

Original languageEnglish
Pages (from-to)654-669
Number of pages16
JournalDevelopmental Biology
Volume165
Issue number2
DOIs
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Xenopus laevis
Acetylation
Histones
trichostatin A
Gene Expression
Xenopus
Gastrulation
Histone Deacetylase Inhibitors
Mesoderm
Morphogenesis
Cell Division
Transcriptional Activation
Oocytes
Embryonic Development
Cell Differentiation
Embryonic Structures
Cell Line

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Histone acetylation influences both gene expression and development of Xenopus laevis. / Almouzni, Geneviève; Khochbin, Saadi; Dimitrov, Stefan; Wolffe, Alan P.

In: Developmental Biology, Vol. 165, No. 2, 1994, p. 654-669.

Research output: Contribution to journalArticle

Almouzni, Geneviève ; Khochbin, Saadi ; Dimitrov, Stefan ; Wolffe, Alan P. / Histone acetylation influences both gene expression and development of Xenopus laevis. In: Developmental Biology. 1994 ; Vol. 165, No. 2. pp. 654-669.
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