Abstract
We demonstrate using a dinucleosome template that acetylation of the core histones enhances transcription by RNA polymerase III. This effect is not dependent on an increased mobility of the core histone octamer with respect to DNA sequence. When linker histone is subsequently bound, we find both a reduction in nucleosome mobility and a repression of transcription. These effects of linker histone binding are independent of core histone acetylation, indicating that core histone acetylation does not prevent linker histone binding and the concomitant transcriptional repression. These studies are complemented by the use of a Xenopus egg extract competent both for chromatin assembly on replicating DNA and for RNA polymerase III transcription. Incorporation of acetylated histones and lack of linker histones together facilitate transcription by > 10-fold in this system; however, they have little independent effect on transcription. Thus core histone acetylation significantly facilitates transcription, but this effect is inhibited by the assembly of linker histones into chromatin.
| Original language | English |
|---|---|
| Pages (from-to) | 2096-2107 |
| Number of pages | 12 |
| Journal | EMBO Journal |
| Volume | 16 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 15 Apr 1997 |
| Externally published | Yes |
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Keywords
- Acetylation
- Histones
- Nucleosome mobility
- Nucleosome positioning
- Transcriptional regulation
ASJC Scopus subject areas
- Neuroscience(all)
- Molecular Biology
- Immunology and Microbiology(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Histone acetylation : Influence on transcription, nucleosome mobility and positioning, and linker histone-dependent transcriptional repression. / Ura, Kiyoe; Kurumizaka, Hitoshi; Dimitrov, Stefan; Almouzni, Geneviève; Wolffe, Alan P.
In: EMBO Journal, Vol. 16, No. 8, 15.04.1997, p. 2096-2107.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Histone acetylation
T2 - Influence on transcription, nucleosome mobility and positioning, and linker histone-dependent transcriptional repression
AU - Ura, Kiyoe
AU - Kurumizaka, Hitoshi
AU - Dimitrov, Stefan
AU - Almouzni, Geneviève
AU - Wolffe, Alan P.
PY - 1997/4/15
Y1 - 1997/4/15
N2 - We demonstrate using a dinucleosome template that acetylation of the core histones enhances transcription by RNA polymerase III. This effect is not dependent on an increased mobility of the core histone octamer with respect to DNA sequence. When linker histone is subsequently bound, we find both a reduction in nucleosome mobility and a repression of transcription. These effects of linker histone binding are independent of core histone acetylation, indicating that core histone acetylation does not prevent linker histone binding and the concomitant transcriptional repression. These studies are complemented by the use of a Xenopus egg extract competent both for chromatin assembly on replicating DNA and for RNA polymerase III transcription. Incorporation of acetylated histones and lack of linker histones together facilitate transcription by > 10-fold in this system; however, they have little independent effect on transcription. Thus core histone acetylation significantly facilitates transcription, but this effect is inhibited by the assembly of linker histones into chromatin.
AB - We demonstrate using a dinucleosome template that acetylation of the core histones enhances transcription by RNA polymerase III. This effect is not dependent on an increased mobility of the core histone octamer with respect to DNA sequence. When linker histone is subsequently bound, we find both a reduction in nucleosome mobility and a repression of transcription. These effects of linker histone binding are independent of core histone acetylation, indicating that core histone acetylation does not prevent linker histone binding and the concomitant transcriptional repression. These studies are complemented by the use of a Xenopus egg extract competent both for chromatin assembly on replicating DNA and for RNA polymerase III transcription. Incorporation of acetylated histones and lack of linker histones together facilitate transcription by > 10-fold in this system; however, they have little independent effect on transcription. Thus core histone acetylation significantly facilitates transcription, but this effect is inhibited by the assembly of linker histones into chromatin.
KW - Acetylation
KW - Histones
KW - Nucleosome mobility
KW - Nucleosome positioning
KW - Transcriptional regulation
UR - http://www.scopus.com/inward/record.url?scp=0030998534&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030998534&partnerID=8YFLogxK
U2 - 10.1093/emboj/16.8.2096
DO - 10.1093/emboj/16.8.2096
M3 - Article
C2 - 9155035
AN - SCOPUS:0030998534
VL - 16
SP - 2096
EP - 2107
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 8
ER -