Higher order folding of chromatin is induced in different ways by monovalent and by bivalent cations

V. L. Makarov, I. Smirnov, S. I. Dimitrov

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The condensation of the 10 nm chromatin filament in the 30 nm fiber by monovalent cations, polyamines and bivalent cations was studied with light scattering at 90° and flow linear dichroism methods. It was found that monovalent cation- and polyamine-induced folding was a two-step process: a precondensation, when a rotation of nucleosomes takes place only, and a condensation step without changes in nucleosome orientation. Divalent cations affected the structure of chromatin in one step only - condensation of the chromatin filament being accompanied by nucleosome reorientation.

Original languageEnglish
Pages (from-to)263-266
Number of pages4
JournalFEBS Letters
Volume212
Issue number2
DOIs
Publication statusPublished - 23 Feb 1987
Externally publishedYes

Fingerprint

Monovalent Cations
Nucleosomes
Chromatin
Cations
Condensation
Polyamines
Divalent Cations
Light scattering
Light
Fibers

Keywords

  • Chromatin structure
  • Flow linear dichroism
  • Light scattering

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Higher order folding of chromatin is induced in different ways by monovalent and by bivalent cations. / Makarov, V. L.; Smirnov, I.; Dimitrov, S. I.

In: FEBS Letters, Vol. 212, No. 2, 23.02.1987, p. 263-266.

Research output: Contribution to journalArticle

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