Higher order folding of chromatin is induced in different ways by monovalent and by bivalent cations

V. L. Makarov, I. Smirnov, S. I. Dimitrov

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The condensation of the 10 nm chromatin filament in the 30 nm fiber by monovalent cations, polyamines and bivalent cations was studied with light scattering at 90° and flow linear dichroism methods. It was found that monovalent cation- and polyamine-induced folding was a two-step process: a precondensation, when a rotation of nucleosomes takes place only, and a condensation step without changes in nucleosome orientation. Divalent cations affected the structure of chromatin in one step only - condensation of the chromatin filament being accompanied by nucleosome reorientation.

Original languageEnglish
Pages (from-to)263-266
Number of pages4
JournalFEBS Letters
Volume212
Issue number2
DOIs
Publication statusPublished - 23 Feb 1987

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Keywords

  • Chromatin structure
  • Flow linear dichroism
  • Light scattering

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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