Higher order folding of chromatin is induced in different ways by monovalent and by bivalent cations

V. L. Makarov, I. Smirnov, S. I. Dimitrov

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The condensation of the 10 nm chromatin filament in the 30 nm fiber by monovalent cations, polyamines and bivalent cations was studied with light scattering at 90° and flow linear dichroism methods. It was found that monovalent cation- and polyamine-induced folding was a two-step process: a precondensation, when a rotation of nucleosomes takes place only, and a condensation step without changes in nucleosome orientation. Divalent cations affected the structure of chromatin in one step only - condensation of the chromatin filament being accompanied by nucleosome reorientation.

Original languageEnglish
Pages (from-to)263-266
Number of pages4
JournalFEBS Letters
Issue number2
Publication statusPublished - 23 Feb 1987



  • Chromatin structure
  • Flow linear dichroism
  • Light scattering

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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