Higher concentrations of histone macroH2A in the Barr body are correlated with higher nucleosome density

Pierre Yves Perche, Claire Vourc'h, Lara Konecny, Catherine Souchier, Michel Robert-Nicoud, Stefan Dimitrov, Saadi Khochbin

Research output: Contribution to journalArticle

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Abstract

Histone macroH2A, which is a subtype of histone H2A, possesses a histone H2A-like portion fused to a relatively long non-histone portion. MacroH2A has been shown to associate preferentially with the inactive X chromosome [1]. To investigate the specificity of this association, the nuclear distribution of macroH2A was compared with that of regular core histones. In normal human female fibroblasts, all anti-histone antibodies that were tested (including anti-macroH2A antibody) preferentially labeled the inactive X chromosome. Moreover, when expressed as green fluorescent protein (GFP) fusions, both histone H2A and macroH2A were concentrated in the Barr body. These data clearly show the presence of a higher density of nucleosomes in the inactive X chromosome. Accordingly, the specificity of the macroH2A association with the inactive X chromosome should be reconsidered. While investigating the role of macroH2A, we found that the proximity of the non-histone region of macroH2A to a promoter could lead to a specific repression of transcription, suggesting that the incorporation of macroH2A into chromatin might help to establish the stable pattern of gene expression in differentiated cells.

Original languageEnglish
Pages (from-to)1531-1534
Number of pages4
JournalCurrent Biology
Volume10
Issue number23
DOIs
Publication statusPublished - 30 Nov 2000
Externally publishedYes

Fingerprint

Sex Chromatin
nucleosomes
Nucleosomes
X Chromosome
histones
Histones
Chromosomes
X chromosome
Anti-Idiotypic Antibodies
Chromosomes, Human, Pair 1
Green Fluorescent Proteins
Antibodies
Chromatin
Transcription
Fibroblasts
antibodies
Gene expression
green fluorescent protein
Gene Expression
Fusion reactions

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Perche, P. Y., Vourc'h, C., Konecny, L., Souchier, C., Robert-Nicoud, M., Dimitrov, S., & Khochbin, S. (2000). Higher concentrations of histone macroH2A in the Barr body are correlated with higher nucleosome density. Current Biology, 10(23), 1531-1534. https://doi.org/10.1016/S0960-9822(00)00832-0

Higher concentrations of histone macroH2A in the Barr body are correlated with higher nucleosome density. / Perche, Pierre Yves; Vourc'h, Claire; Konecny, Lara; Souchier, Catherine; Robert-Nicoud, Michel; Dimitrov, Stefan; Khochbin, Saadi.

In: Current Biology, Vol. 10, No. 23, 30.11.2000, p. 1531-1534.

Research output: Contribution to journalArticle

Perche, PY, Vourc'h, C, Konecny, L, Souchier, C, Robert-Nicoud, M, Dimitrov, S & Khochbin, S 2000, 'Higher concentrations of histone macroH2A in the Barr body are correlated with higher nucleosome density', Current Biology, vol. 10, no. 23, pp. 1531-1534. https://doi.org/10.1016/S0960-9822(00)00832-0
Perche, Pierre Yves ; Vourc'h, Claire ; Konecny, Lara ; Souchier, Catherine ; Robert-Nicoud, Michel ; Dimitrov, Stefan ; Khochbin, Saadi. / Higher concentrations of histone macroH2A in the Barr body are correlated with higher nucleosome density. In: Current Biology. 2000 ; Vol. 10, No. 23. pp. 1531-1534.
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